LOCUS       Exported                 806 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  Tet on shKras for conditional Kras knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    TGMP.shKras.1422
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Shao DD, Xue W, Krall EB, Bhutkar A, Piccioni F, Wang X, Schinzel 
            AC, Sood S, Rosenbluh J, Kim JW, Zwang Y, Roberts TM, Root DE, Jacks
            T, Hahn WC
  TITLE     KRAS and YAP1 Converge to Regulate EMT and Tumor Survival.
  JOURNAL   Cell. 2014 Jul 3;158(1):171-84. doi: 10.1016/j.cell.2014.06.004. 
            Epub 2014 Jun 19.
  PUBMED    24954536
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     12..35
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     ncRNA           complement(84..210)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(238..252)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(280..401)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(490..511)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcgccagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg gaaaagcgcc
       61 tcccctaccc ggtagaattg aaaaaagtga tttaatttat accattttaa ttcagctttg
      121 taaaaatgta tcaaagagat agcaaggtat tcagttttag taaacaagat aattgctcct
      181 aaagtagccc cttgaattcc gaggcagtag gcagcagacc cagtatgaaa tagtatacat
      241 ctgtggcttc actatactat ttcatactgg gtctgtcgct cactgtcaac agcaatatac
      301 cttctcgagc cttctgttgg gttaacctga agaagtaatc ccagcaagtg tttccaagat
      361 gtgcaggcaa cgattctgta aagtactgaa gcctcattca aacaattacc ctgttatccc
      421 tagtcgaggc ttagccgtac gtcattactt gtacagctcg tccatgccga gagtgatccc
      481 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
      541 cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
      601 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg
      661 gatcttgaag ttcgccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg
      721 gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
      781 gcccttcagc tcgatgcggt tcacca
//
