LOCUS Exported 783 bp ds-DNA linear SYN 20-MAY-2021 DEFINITION Tet on shKras for conditional Kras knockdown. ACCESSION . VERSION . KEYWORDS TGMP.shKras.1422 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 783) AUTHORS Shao DD, Xue W, Krall EB, Bhutkar A, Piccioni F, Wang X, Schinzel AC, Sood S, Rosenbluh J, Kim JW, Zwang Y, Roberts TM, Root DE, Jacks T, Hahn WC TITLE KRAS and YAP1 Converge to Regulate EMT and Tumor Survival. JOURNAL Cell. 2014 Jul 3;158(1):171-84. doi: 10.1016/j.cell.2014.06.004. Epub 2014 Jun 19. PUBMED 24954536 REFERENCE 2 (bases 1 to 783) AUTHORS . TITLE Direct Submission JOURNAL Exported May 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..783 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 37..636 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(37..56) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 533..553 /label=Puro-F /note="Puromycin resistance gene, forward primer" ORIGIN 1 atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg 61 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 121 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 181 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 241 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 301 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 361 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 421 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 481 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 541 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 601 cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc 661 ccgaccgaaa ggagcgcacg accccatgca tcgataaaat aaaagatttt atttagtctc 721 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctaga gaaccatcag 781 atg //