LOCUS Exported 9366 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pHM830 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9366) AUTHORS Sorg G, Stamminger T TITLE Mapping of nuclear localization signals by simultaneous fusion to green fluorescent protein and to beta-galactosidase. JOURNAL Biotechniques. 1999 May . 26(5):858-62. PUBMED 10337476 REFERENCE 2 (bases 1 to 9366) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9366 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 904..1617 /codon_start=1 /product="green fluorescent protein" /label=GFP /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" CDS 1849..4896 /codon_start=1 /gene="lacZ" /product="beta-galactosidase" /label=lacZ /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD SWSPSVSAEFQLSAGRYHYQLVWCQK" primer_bind complement(1851..1873) /label=M13/pUC Forward /note="In lacZ gene" promoter complement(4918..4936) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(4919..4936) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(4956..4973) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4962..5186 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5232..5660 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5319..5338) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5529..5550 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5669..5689) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5674..6004 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5855..5990 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5917..5936 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 6071..6865 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6125..6144) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 6735..6754 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 7039..7160 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7076..7095) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7130..7149 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7209..7225) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7209..7225) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7222..7244) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7233..7249 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7257..7287) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7302..7323 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7440..7457) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7611..8199) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7691..7710) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8370..9230) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8993..9012 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9231..9335) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccgcc 901 accatgagca agggcgagga actgttcact ggcgtggtcc caattctcgt ggaactggat 961 ggcgatgtga atgggcacaa attttctgtc agcggagagg gtgaaggtga tgccacatac 1021 ggaaagctca ccctgaaatt catctgcacc actggaaagc tccctgtgcc atggccaaca 1081 ctggtcacta ccctgaccta tggcgtgcag tgcttttcca gatacccaga ccatatgaag 1141 cagcatgact ttttcaagag cgccatgccc gagggctatg tgcaggagag aaccatcttt 1201 ttcaaagatg acgggaacta caagacccgc gctgaagtca agttcgaagg tgacaccctg 1261 gtgaatagaa tcgagctgaa gggcattgac tttaaggagg atggaaacat tctcggccac 1321 aagctggaat acaactataa ctcccacaat gtgtacatca tggccgacaa gcaaaagaat 1381 ggcatcaagg tcaacttcaa gatcagacac aacattgagg atggatccgt gcagctggcc 1441 gaccattatc aacagaacac tccaatcggc gacggccctg tgctcctccc agacaaccat 1501 tacctgtcca cccagtctgc cctgtctaaa gatcccaacg aaaagagaga ccacatggtc 1561 ctgctggagt ttgtgaccgc tgctgggatc acacatggca tggacgagct gtacaaggct 1621 atcgatgctc tcgacgctag gcccgggccc gctagcggcc ttaagggccc gcggggccac 1681 gtgggctcta gagggcccgg gcctagaatg ccttctgaac aatggaaagg cattattgcc 1741 gtaagccgtg gcggtctgat accggtgggt gaagaccaga aacagcacct cgaactgagc 1801 cgcgatattg cccagcgttt caacgcgctg tatggcgaga tcgatcccgt cgttttacaa 1861 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct 1921 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 1981 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc 2041 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg 2101 cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg 2161 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc 2221 tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg 2281 tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac 2341 ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt 2401 gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc 2461 tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat 2521 gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac 2581 tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg 2641 cctttcggcg gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt 2701 ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg 2761 gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc 2821 cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga 2881 ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg 2941 gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat 3001 ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa 3061 gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg 3121 ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt 3181 gtgatcatct ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat 3241 cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac 3301 accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc 3361 ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg 3421 ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac 3481 tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat 3541 cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt 3601 ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg 3661 ccgcatccag cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc 3721 gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg 3781 cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc 3841 gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg 3901 caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg 3961 cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc 4021 gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt 4081 aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat 4141 aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac 4201 attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg 4261 gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat 4321 gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc 4381 cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg 4441 gcgagcgata caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca 4501 gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc 4561 gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg 4621 agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc 4681 ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat 4741 cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg 4801 attggtggcg acgactcctg gagcccgtca gtatcggcgg aattccagct gagcgccggt 4861 cgctaccatt accagttggt ctggtgtcaa aaataactcg agactagagg gccctattct 4921 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca 4981 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 5041 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 5101 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 5161 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 5221 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 5281 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 5341 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg 5401 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 5461 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 5521 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 5581 ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta 5641 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 5701 ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 5761 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 5821 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 5881 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 5941 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 6001 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg 6061 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 6121 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 6181 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 6241 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 6301 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 6361 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 6421 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 6481 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 6541 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 6601 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 6661 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 6721 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 6781 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 6841 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 6901 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 6961 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 7021 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 7081 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 7141 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 7201 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 7261 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 7321 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 7381 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 7441 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 7501 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 7561 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 7621 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 7681 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 7741 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 7801 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 7861 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 7921 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 7981 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 8041 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 8101 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 8161 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 8221 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 8281 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 8341 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 8401 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 8461 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 8521 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 8581 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 8641 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 8701 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 8761 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 8821 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 8881 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 8941 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 9001 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 9061 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 9121 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 9181 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 9241 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 9301 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 9361 gacgtc //