LOCUS       Exported                 805 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  Tet on shKras for conditional Kras knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    TGMP.shKras.1422
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Shao DD, Xue W, Krall EB, Bhutkar A, Piccioni F, Wang X, Schinzel 
            AC, Sood S, Rosenbluh J, Kim JW, Zwang Y, Roberts TM, Root DE, Jacks
            T, Hahn WC
  TITLE     KRAS and YAP1 Converge to Regulate EMT and Tumor Survival.
  JOURNAL   Cell. 2014 Jul 3;158(1):171-84. doi: 10.1016/j.cell.2014.06.004. 
            Epub 2014 Jun 19.
  PUBMED    24954536
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(89..113)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(110..148)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    181..451
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(515..531)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(547..569)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ttgctcacca tggtggatcc gtcgagaggc tggatcggtc ccggtgtctt ctatggaggt
       61 caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga gctctgctta
      121 tataggcctc ccaccgtaca cgcctacctc gacccgggta ccgagctcga ctttcacttt
      181 tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc actgataggg
      241 agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt
      301 cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg
      361 atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc
      421 gactttcact tttctctatc actgataggg agtggtaaac tcgatgctct agaggatcga
      481 tcccccagat ctaattccgg cgcctagaga aggagtgagg gctggataaa gggaggatcg
      541 aggcggggtc gaacgaggag gttcaagggg gagagacggg gcggatggag gaagaggagg
      601 cggaggctta gggtgtacaa agggcttgac ccagggaggg gggtcaaaag ccaaggcttc
      661 ccaggtcacg atgtagggga cctggtctgg gtgtccatgc gggccaggtg aaaagacctt
      721 gatcttaacc tgggtgatga ggtctcggtt aaaggtgccg tctcgcggcc atccgacgtt
      781 aaaggttggc cattctgcag agcag
//
