LOCUS       Exported                8860 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Human dysferlin exon 1 variant (accession #DQ267935) cDNA in 
            pDONR221 plasmid which can be used as an entry vector for 
            Invitrogen's gateway system..
ACCESSION   .
VERSION     .
KEYWORDS    Human-Dysferlin-v1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8860)
  TITLE     Human dysferlin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8860
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1385..1396
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6985..7084)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7101..7120)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7102..7120)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7125..7141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7125..7141)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7243..7262)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7254..8063
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7321..7340)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8210..8798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8699..8718
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctca ctagtggtac cgtttaaacg atcggccgcc accatgctgt gctgcctgct
      721 ggtgagggcc agcaacctcc ccagtgcgaa gaaggaccgg cgcagcgacc ctgtcgcaag
      781 cctgactttc cgaggggtga agaagagaac caaagtcatc aagaacagcg tgaaccctgt
      841 atggaatgag ggatttgaat gggacctcaa gggcatcccc ctggaccagg gctctgagct
      901 tcatgtggtg gtcaaagacc atgagacgat ggggaggaac aggttcctgg gggaagccaa
      961 ggtcccactc cgagaggtcc tcgccacccc tagtctgtcc gccagcttca atgcccccct
     1021 gctggacacc aagaagcagc ccacaggggc ctcgctggtc ctgcaggtgt cctacacacc
     1081 gctgcctgga gctgtgcccc tgttcccgcc ccctactcct ctggagccct ccccgactct
     1141 gcctgacctg gatgtagtgg cagacacagg aggagaggaa gacacagagg accagggact
     1201 cactggagat gaggcggagc cattcctgga tcaaagcgga ggcccggggg ctcccaccac
     1261 cccaaggaaa ctaccttcac gtcctccgcc ccactacccc gggatcaaaa gaaagcgaag
     1321 tgcgcctaca tctagaaagc tgctgtcaga caaaccgcag gatttccaga tcagggtcca
     1381 ggtgatcgag gggcgccagc tgccgggggt gaacatcaag cctgtggtca aggttaccgc
     1441 tgcagggcag accaagcgga cgcggatcca caagggaaac agcccactct tcaatgagac
     1501 tcttttcttc aacttgtttg actctcctgg ggagctgttt gatgagccca tctttatcac
     1561 ggtggtagac tctcgttctc tcaggacaga tgctctcctc ggggagttcc ggatggacgt
     1621 gggcaccatt tacagagagc cccggcacgc ctatctcagg aagtggctgc tgctctcaga
     1681 ccctgatgac ttctctgctg gggccagagg ctacctgaaa acaagccttt gtgtgctggg
     1741 gcctggggac gaagcgcctc tggagagaaa agacccctct gaagacaagg aggacattga
     1801 aagcaacctg ctccggccca caggcgtagc cctgcgagga gcccacttct gcctgaaggt
     1861 cttccgggcc gaggacttgc cgcagatgga cgatgccgtg atggacaacg tgaaacagat
     1921 ctttggcttc gagagtaaca agaagaactt ggtggacccc tttgtggagg tcagctttgc
     1981 ggggaaaatg ctgtgcagca agatcttgga gaagacggcc aaccctcagt ggaaccagaa
     2041 catcacactg cctgccatgt ttccctccat gtgcgaaaaa atgaggattc gtatcataga
     2101 ctgggaccgc ctgactcaca atgacatcgt ggctaccacc tacctgagta tgtcgaaaat
     2161 ctctgcccct ggaggagaaa tagaagagga gcctgcaggt gctgtcaagc cttcgaaagc
     2221 ctcagacttg gatgactacc tgggcttcct ccccactttt gggccctgct acatcaacct
     2281 ctatggcagt cccagagagt tcacaggctt cccagacccc tacacagagc tcaacacagg
     2341 caagggggaa ggtgtggctt atcgtggccg gcttctgctc tccctggaga ccaagctggt
     2401 ggagcacagt gaacagaagg tggaggacct tcctgcggat gacatcctcc gggtggagaa
     2461 gtaccttagg aggcgcaagt actccctgtt tgcggccttc tactcagcca ccatgctgca
     2521 ggatgtggat gacgccatcc agtttgaggt cagcatcggg aactacggga acaagttcga
     2581 catgacctgc ctgccgctgg cctccaccac tcagtacagc cgtgcagtct ttgacgggtg
     2641 ccactactac tacctaccct ggggtaacgt gaaacctgtg gtggtgctgt catcctactg
     2701 ggaggacatc agccatagaa tcgagactca gaaccagctg cttgggattg ctgaccggct
     2761 ggaagctggc ctggagcagg tccacctggc cctgaaggcg cagtgctcca cggaggacgt
     2821 ggactcgctg gtggctcagc tgacggatga gctcatcgca ggctgcagcc agcctctggg
     2881 tgacatccat gagacaccct ctgccaccca cctggaccag tacctgtacc agctgcgcac
     2941 ccatcacctg agccaaatca ctgaggctgc cctggccctg aagctcggcc acagtgagct
     3001 ccctgcagct ctggagcagg cggaggactg gctcctgcgt ctgcgtgccc tggcagagga
     3061 gccccagaac agcctgccgg acatcgtcat ctggatgctg cagggagaca agcgtgtggc
     3121 ataccagcgg gtgcccgccc accaagtcct cttctcccgg cggggtgcca actactgtgg
     3181 caagaattgt gggaagctac agacaatctt tctgaaatat ccgatggaga aggtgcctgg
     3241 cgcccggatg ccagtgcaga tacgggtcaa gctgtggttt gggctctctg tggatgagaa
     3301 ggagttcaac cagtttgctg aggggaagct gtctgtcttt gctgaaacct atgagaacga
     3361 gactaagttg gcccttgttg ggaactgggg cacaacgggc ctcacctacc ccaagttttc
     3421 tgacgtcacg ggcaagatca agctacccaa ggacagcttc cgcccctcgg ccggctggac
     3481 ctgggctgga gattggttcg tgtgtccgga gaagactctg ctccatgaca tggacgccgg
     3541 tcacctgagc ttcgtggaag aggtgtttga gaaccagacc cggcttcccg gaggccagtg
     3601 gatctacatg agtgacaact acaccgatgt gaacggggag aaggtgcttc ccaaggatga
     3661 cattgagtgc ccactgggct ggaagtggga agatgaggaa tggtccacag acctcaaccg
     3721 ggctgtcgat gagcaaggct gggagtatag catcaccatc cccccggagc ggaagccgaa
     3781 gcactgggtc cctgctgaga agatgtacta cacacaccga cggcggcgct gggtgcgcct
     3841 gcgcaggagg gatctcagcc aaatggaagc actgaaaagg cacaggcagg cggaggcgga
     3901 gggcgagggc tgggagtacg cctctctttt tggctggaag ttccacctcg agtaccgcaa
     3961 gacagatgcc ttccgccgcc gccgctggcg ccgtcgcatg gagccactgg agaagacggg
     4021 gcctgcagct gtgtttgccc ttgagggggc cctgggcggc gtgatggatg acaagagtga
     4081 agattccatg tccgtctcca ccttgagctt cggtgtgaac agacccacga tttcctgcat
     4141 attcgactat gggaaccgct accatctacg ctgctacatg taccaggccc gggacctggc
     4201 tgcgatggac aaggactctt tttctgatcc ctatgccatt gtctccttcc tgcaccagag
     4261 ccagaagacg gtggtggtga agaacaccct taaccccacc tgggaccaga cgctcatctt
     4321 ctacgagatc gagatctttg gcgagccggc cacagttgct gagcaaccgc ccagcattgt
     4381 ggtggagctg tacgaccatg acacttatgg tgcagacgag tttatgggtc gctgcatctg
     4441 tcaaccgagt ctggaacgga tgccacggct ggcctggttc ccactgacga ggggcagcca
     4501 gccgtcgggg gagctgctgg cctcttttga gctcatccag agagagaagc cggccatcca
     4561 ccatattcct ggttttgagg tgcaggagac atcaaggatc ctggatgagt ctgaggacac
     4621 agacctgccc tacccaccac cccagaggga ggccaacatc tacatggttc ctcagaacat
     4681 caagccagcg ctccagcgta ccgccatcga gatactggca tggggcctgc ggaacatgaa
     4741 gagttaccag ctggccaaca tctcctcccc cagcctcgtg gtagagtgtg ggggccagac
     4801 ggtgcagtcc tgtgtcatca ggaacctccg gaagaacccc aactttgaca tctgcaccct
     4861 cttcatggaa gtgatgctgc ccagggagga gctctactgc ccccccatca ccgtcaaggt
     4921 catcgataac cgccagtttg gccgccggcc tgtggtgggc cagtgtacca tccgctccct
     4981 ggagagcttc ctgtgtgacc cctactcggc ggagagtcca tccccacagg gtggcccaga
     5041 cgatgtgagc ctactcagtc ctggggaaga cgtgctcatc gacattgatg acaaggagcc
     5101 cctcatcccc atccaggagg aagagttcat cgattggtgg agcaaattct ttgcctccat
     5161 aggggagagg gaaaagtgcg gctcctacct ggagaaggat tttgacacct tgaaggtcta
     5221 tgacacacag ctggagaatg tggaggcctt tgagggcctg tctgactttt gtaacacctt
     5281 caagctgtac cggggcaaga cgcaggagga gacagaagat ccatctgtga ttggtgaatt
     5341 taagggcctc ttcaaaattt atcccctccc agaagaccca gccatcccca tgcccccaag
     5401 acagttccac cagctggccg cccagggacc ccaggagtgc ttggtccgta tctacattgt
     5461 ccgagcattt ggcctgcagc ccaaggaccc caatggaaag tgtgatcctt acatcaagat
     5521 ctccataggg aagaaatcag tgagtgacca ggataactac atcccctgca cgctggagcc
     5581 cgtatttgga aagatgttcg agctgacctg cactctgcct ctggagaagg acctaaagat
     5641 cactctctat gactatgacc tcctctccaa ggacgaaaag atcggtgaga cggtcgtcga
     5701 cctggagaac aggctgctgt ccaagtttgg ggctcgctgt ggactcccac agacctactg
     5761 tgtctctgga ccgaaccagt ggcgggacca gctccgcccc tcccagctcc tccacctctt
     5821 ctgccagcag catagagtca aggcacctgt gtaccggaca gaccgtgtaa tgtttcagga
     5881 taaagaatat tccattgaag agatagaggc tggcaggatc ccaaacccac acctgggccc
     5941 agtggaggag cgtctggctc tgcatgtgct tcagcagcag ggcctggtcc cggagcacgt
     6001 ggagtcacgg cccctctaca gccccctgca gccagacatc gagcagggga agctgcagat
     6061 gtgggtcgac ctatttccga aggccctggg gcggcctgga cctcccttca acatcacccc
     6121 acggagagcc agaaggtttt tcctgcgttg tattatctgg aataccagag atgtgatcct
     6181 ggatgacctg agcctcacgg gggagaagat gagcgacatt tatgtgaaag gttggatgat
     6241 tggctttgaa gaacacaagc aaaagacaga cgtgcattat cgttccctgg gaggtgaagg
     6301 caacttcaac tggaggttca ttttcccctt cgactacctg ccagctgagc aagtctgtac
     6361 cattgccaag aaggatgcct tctggaggct ggacaagact gagagcaaaa tcccagcacg
     6421 agtggtgttc cagatctggg acaatgacaa gttctccttt gatgattttc tgggctccct
     6481 gcagctcgat ctcaaccgca tgcccaagcc agccaagaca gccaagaagt gctccttgga
     6541 ccagctggat gatgctttcc acccagaatg gtttgtgtcc ctttttgagc agaaaacagt
     6601 gaagggctgg tggccctgtg tagcagaaga gggtgagaag aaaatactgg cgggcaagct
     6661 ggaaatgacc ttggagattg tagcagagag tgagcatgag gagcggcctg ctggccaggg
     6721 ccgggatgag cccaacatga accctaagct tgaggaccca aggcgccccg acacctcctt
     6781 cctgtggttt acctccccat acaagaccat gaagttcatc ctgtggcggc gtttccggtg
     6841 ggccatcatc ctcttcatca tcctcttcat cctgctgctg ttcctggcca tcttcatcta
     6901 cgccttcccg aactatgctg ccatgaagct ggtgaagccc ttcagctgag catatgttaa
     6961 ttaacctagg ttataagcgg ccgcacccag ctttcttgta caaagttggc attataagaa
     7021 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     7081 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     7141 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaataatat
     7201 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     7261 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
     7321 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
     7381 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     7441 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     7501 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
     7561 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     7621 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     7681 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     7741 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     7801 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     7861 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     7921 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     7981 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     8041 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
     8101 ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg
     8161 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8221 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8281 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8341 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8401 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8461 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8521 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8581 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8641 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8701 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8761 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8821 tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
