LOCUS Exported 853 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Expresses RNF4 in mammalian cells. ACCESSION . VERSION . KEYWORDS HA-RNF4/pRK5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 853) AUTHORS Guo L, Giasson BI, Glavis-Bloom A, Brewer MD, Shorter J, Gitler AD, Yang X TITLE A Cellular System that Degrades Misfolded Proteins and Protects against Neurodegeneration. JOURNAL Mol Cell. 2014 Jul 3;55(1):15-30. doi: 10.1016/j.molcel.2014.04.030. Epub 2014 May 29. PUBMED 24882209 REFERENCE 2 (bases 1 to 853) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..853 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 55..81 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 670..693 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" regulatory 704..713 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 717..851 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(754..773) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 808..827 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tccactccca ggtccaactg cacctcggtt ctatcgattg aattcgccat gggctacccc 61 tacgacgtgc ccgactacgc cagatccatg agtacaagaa agcgtcgtgg tggagcaata 121 aattctagac aagctcagaa gcgaactcgg gaagcaacct ccacccccga gatctccttg 181 gaagcagaac ccatagaact cgtggaaact gctggagatg aaattgtgga cctcacttgt 241 gaatctttag agcctgtggt ggttgatctg actcacaatg actctgttgt gattgttgac 301 gaaagaagaa gaccaaggag gaatgctagg aggctgcccc aggaccatgc tgacagctgt 361 gtggtgagca gtgacgatga ggagttgtcc agggacagag acgtatatgt gactacccat 421 actcccagaa acgccaggga tgagggcgct acaggcctca ggccctcagg tactgtcagt 481 tgtcccatct gcatggacgg atactcagag atcgtgcaga atggacgtct catcgtttcc 541 acagaatgcg gccatgtctt ctgtagccag tgcctccgtg attccctgaa gaatgctaat 601 acttgcccaa cttgtaggaa aaagatcaac cacaaacggt accaccccat ttatatagtc 661 gactgactcg actacaagga cgacgatgac aagtgaaagc ttggccgcca tggcccaact 721 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 781 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 841 atgtctggat cgg //