LOCUS Exported 3306 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.. ACCESSION . VERSION . KEYWORDS pENTR D-TOPO-C3_5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3306) AUTHORS Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J TITLE Pancreatic islet enhancer clusters enriched in type 2 diabetes risk-associated variants. JOURNAL Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 Jan 12. PUBMED 24413736 REFERENCE 2 (bases 1 to 3306) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3306 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1431..1530) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1547..1566) /label=T7 /note="T7 promoter, forward primer" promoter complement(1548..1566) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1571..1587) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1571..1587) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1689..1708) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1700..2509 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1767..1786) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2656..3244 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3145..3164 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc gcggccgccc ccttcacccc atcacctgtg accttttttc ttcttttctt 721 ttgttagaga cagggtctcg ctatgttgcc caggctgatc tctaactcct gggctcaaga 781 gattcccccg cctcagcctc cggtgtagct gggagtacag gtgcacatca ccatgcctgg 841 caagactgtg tcttttcaat ggccttttca tctctatgtc cccagtgcct gatacaggga 901 agccctctca gaaatgaagg gaaggaaata aagaaaagga ggaataaagc cagggaatgg 961 tttcctttct cattcaggcc acctctcatt actagggcct gagactgctg aagggcatta 1021 gtgactgaac atgttgacat tgtgtaattt attagcctga actgttcaaa ttatgccttc 1081 cccccaggac ttgcccgcaa tgaaagcaga ttttatcccg gggccctgaa taaatactca 1141 gagaaacaga aacctcatga attttttaaa gagcttattg aaatcaggga aaaacctggc 1201 gttctgagtc agaagtatcc agtacatcag cattcgtttc tctttaccct ctttaatctt 1261 ttttaatccc cttgctttcc agtattaatc tgtgcttctc cccccgaatt cccctccatt 1321 ttggatcatc cagagaaaaa taacccaccc acaagactcc cacctccatc tcccatcata 1381 ctggcaggac tggggaatgt tgcccagccc ctgtaagggt gggcgcgccg acccagcttt 1441 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt 1501 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc 1561 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg 1621 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa 1681 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa 1741 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc 1801 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca 1861 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac 1921 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt 1981 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc 2041 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt 2101 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat 2161 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga 2221 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca 2281 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga 2341 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct 2401 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc 2461 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt 2521 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg 2581 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa 2641 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 2701 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 2761 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 2821 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 2881 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 2941 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 3001 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc 3061 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 3121 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 3181 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 3241 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 3301 catgtt //