LOCUS       Exported                 704 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  Allows the integration of Myc ArA K162R in the genome and 
            Tet-inducible expression.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA5 FRT TO Myc ArA K162R
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Girdler F, Gascoigne KE, Eyers PA, Hartmuth S, Crafter C, Foote KM, 
            Keen NJ, Taylor SS
  TITLE     Validating Aurora B as an anti-cancer drug target.
  JOURNAL   J Cell Sci. 2006 Sep 1;119(Pt 17):3664-75. Epub 2006 Aug 15.
  PUBMED    16912073
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     37..61
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             322..351
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat
       61 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      121 cctccggact ctagcgttta aacttaagct tggtaccccg gggaattcgg cagatcccga
      181 ggtccgacag cgcccggccc agatccccac gcctgccagg agcaagccga gagccagccg
      241 gccggcgcac tccgactccg agcagtctct gtccttcgac ccgagccccg cgccctttcc
      301 gggacccctg ccccgcggat ggagcagaag ctcatctcag aagaagacct cgagggatcc
      361 gaccgatcta aagaaaactg catttcagga cctgttaagg ctacagctcc agttggaggt
      421 ccaaaacgtg ttctcgtgac tcagcaattt ccttgtcaga atccattacc tgtaaatagt
      481 ggccaggctc agcgggtctt gtgtccttca aattcttccc agcgcgttcc tttgcaagca
      541 caaaagcttg tctccagtca caagccggtt cagaatcaga agcagaagca attgcaggca
      601 accagtgtac ctcatcctgt ctccaggcca ctgaataaca cccaaaagag caagcagccc
      661 ctgccatcgg cacctgaaaa taatcctgag gaggaactgg catc
//
