LOCUS       Exported                4384 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  This plasmids is a modular mini-Tn5 vector that could be used to 
            generate random mutagenesis libraries or to insert heterologous DNA 
            into a target genome .
ACCESSION   .
VERSION     .
KEYWORDS    pBAM1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Martinez-Garcia E, Calles B, Arevalo-Rodriguez M, de Lorenzo V
  TITLE     pBAM1: an all-synthetic genetic tool for analysis and construction 
            of complex bacterial phenotypes.
  JOURNAL   BMC Microbiol. 2011 Feb 22;11:38. doi: 10.1186/1471-2180-11-38.
  PUBMED    21342504
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      4..392
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            411..519
                     /note="incP origin of transfer"
     terminator      666..705
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(709..1569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1570..1661)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(1718..3145)
                     /codon_start=1
                     /product="transposase from the bacterial Tn5 transposon 
                     (Reznikoff, 1993)"
                     /label=Tn5 transposase
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     misc_feature    3239..3257
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(3354..3373)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3365..4180
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    4267..4323
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    4355..4373
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
ORIGIN
        1 ccatgtcagc cgttaagtgt tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc
       61 ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta aaggctttaa
      121 aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg
      181 ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct
      241 tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg
      301 agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac
      361 tatcaacagg ttgaactgct gatcttcaga tcggcgcgcc ggccggccta cggccagcct
      421 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa
      481 cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac
      541 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga
      601 tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaaggacaag
      661 ggtcgtccaa aaaaaaaggc tccaaaagga gcctttaatt gtatcggttt accaatgttt
      721 aatcaggctc gcgccaattt ccgcaatctg acggttacgt tcatccatcg tcgcctggct
      781 gccggtggta taaatcacca caatacggct cggtttacca tccgggccca gcgccgcaat
      841 aatgccacgg ctgccacgtt cgcccgcacc gcttttatcc gcaataaacc agccagccgg
      901 cagcgcgcta cgcagcagcg gacccgccac tttatccgct tccatccaat caatcagttg
      961 ctggcggctt gccagggtca gcagctcacc cgtcagcagt ttacgcaggg tggtcgccat
     1021 tgctgccggc atggtggtat cacgttcatc gttcggaatc gcttcgttca gttccggttc
     1081 ccaacgatcc agacgggtca catgatcgcc catgttatgc agaaacgcgg tcagttcttt
     1141 cggaccgcca atggtcgtca gcagcaggtt cgccgcggtg ttatcgctca tggtaatcgc
     1201 cgcgctgcac agttcacgca cggtcatgcc atcggtcaga tgtttttcgg tcaccgggct
     1261 atattccacc agatcgttct ggctataatg aatacgacgg cccagttgtt cctggcccgc
     1321 atccacacgg ctcagaaccg caccgcacag cagcacttta aaggtgctca tcatcggaaa
     1381 acgttcttcc ggacgaaaag attccagaat tttgccgctg ttcagatcca gttcaatata
     1441 gcccacgcgc gcacccagtt gatcttccgc atctttcact ttcaccaggg tttccggatg
     1501 cgcaaacacc ggcaggcaaa acgccgcaaa aaacggaatc agcgccacac gaaaatgctg
     1561 aatgctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1621 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggaat taaaaaaaag
     1681 cccgctcatt aggcgggcta ctaatttaaa tcgattagat tttaatgccc tgcgccatca
     1741 ggtctttcgc ggccagaaag ccatccagtt tgctttgcag cgcttcccaa ccttcccaca
     1801 gcgcacccca gctcgcaatg ccggtacgtt tgctatccat aaagccgccc agacgcgcaa
     1861 tcgccatata cgcccattgc aggctgcccg ctttttcttt gcgtttgcgt ttgcctttat
     1921 ccagatagcc cagcagttgg cattcatccg gggtcagcac ggtttccgcg ctctggcttt
     1981 caacgtgttc cgcttctttc agcaggccct gcgcacgcag tgcttgcggc ggagtaaaag
     2041 attcacgcag ttgcagcaga cgcaccgcca caaagctcag aatgctcacc atacgttcca
     2101 ggttatccgg ttcttccata cgctgacgtt ccgcacccgc acccgttttc cacgctttgt
     2161 gaaattcttc aatgcgccaa cgatgggtat aaatatcaat cacacgcagc gcttgggcca
     2221 gactttccac cggctcgctg gtcagcagca gccatttcag cggggtttcg cctttcggcg
     2281 gattaatttc ttcggccagc accgcgttca gggtaatgtt gccctgtttc agggtaatac
     2341 ggccgctacg caggctcagg ctcgctttac gcgccggacg gtttttacgt ttgccacgtt
     2401 tatccaccac gcctttctgc ggaatgctaa tctgatagcc gcccagttcc ggctggtttt
     2461 tcaggtgatc atacagatac aggccgcttt ccacatcttt acgcggatgt ttgctacgca
     2521 ccacaaaacg ttcgttatgg gccagtttat cttgcagata cgcatgaata tccgcttcac
     2581 gatcgcacac cgcaatcacg ttgctcatca tgctgcccat tctcagacgc gaagttgcag
     2641 cagcggccag ccatttgccg ctttcttttt catccgcatc cgccggatca tccggacgca
     2701 tccaccattc ttgatgcagc aggcccacgg tacgaaaggt ggtcgcttcc agcagcagca
     2761 cgctatgcac ccaccaacca cggcttttat cctgaatgct acccagtttg cccagttctt
     2821 ccgccacctg atgacgatag ctcagagagg tggtatcttc aattgccagc agttccggaa
     2881 attcctgggc cagtttcacg gtctgcatgg cacccgcttt acgaatcgct tccgcgctca
     2941 cgttcggatt acgaataaaa cgatacgcgc cttcctgcat ggctttgctg ccttcgctgc
     3001 taatggtaat gcttttgccg ctatatttgg ccagttgcgc cgcaacattc accagacgcg
     3061 cggtacgacg cggatcaccc agcgcagcac tagaaaacac gcttttcgcc caatccgccg
     3121 cacgatgcag tgcactggta atcatgaacg ttaccatgtt aggaggtcac atggaacatc
     3181 agattctgga aaacgggaaa ggttccgttc aggacgctac ttgtgtagtt taaaccagct
     3241 gtctcttata cacatctgac gtcttgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     3301 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     3361 tgttatgagc catattcagc gtgaaacgag ctgtagccgt ccgcgtctga acagcaacat
     3421 ggatgcggat ctgtatggct ataaatgggc gcgtgataac gtgggtcaga gcggcgcgac
     3481 catttatcgt ctgtatggca aaccggatgc gccggaactg tttctgaaac atggcaaagg
     3541 cagcgtggcg aacgatgtga ccgatgaaat ggtgcgtctg aactggctga ccgaatttat
     3601 gccgctgccg accattaaac attttattcg caccccggat gatgcgtggc tgctgaccac
     3661 cgcgattccg ggcaaaaccg cgtttcaggt gctggaagaa tatccggata gcggcgaaaa
     3721 cattgtggat gcgctggccg tgtttctgcg tcgtctgcat agcattccgg tgtgcaactg
     3781 cccgtttaac agcgatcgtg tgtttcgtct ggcccaggcg cagagccgta tgaacaacgg
     3841 cctggtggat gcgagcgatt ttgatgatga acgtaacggc tggccggtgg aacaggtgtg
     3901 gaaagaaatg cataaactgc tgccgtttag cccggatagc gtggtgaccc acggcgattt
     3961 tagcctggat aacctgattt tcgatgaagg caaactgatt ggctgcattg atgtgggccg
     4021 tgtgggcatt gcggatcgtt atcaggatct ggccattctg tggaactgcc tgggcgaatt
     4081 tagcccgagc ctgcaaaaac gtctgtttca gaaatatggc attgataatc cggatatgaa
     4141 caaactgcaa tttcatctga tgctggatga atttttctaa taattaattg gggaccctag
     4201 aggtcccctt ttttatttta aaaatttttt cacaaaacgg tttacaagca taactagtgc
     4261 ggccgcaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa
     4321 ttcgcgcggc cgcggcctag gcggccttaa ttaaagatgt gtataagaga cagctgggcg
     4381 cgcc
//
