LOCUS Exported 779 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters. ACCESSION . VERSION . KEYWORDS pAC154-dual-dCas9VP160-sgExpression SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 779) AUTHORS Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R TITLE Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. JOURNAL Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. PUBMED 23979020 REFERENCE 2 (bases 1 to 779) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..779 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(9..392) /codon_start=1 /product="10 tandem repeats of the minimal activation domain of herpes simplex virus VP16 (Cheng et al., 2013)" /label=VP160 /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF DLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGS DALDDFDLDMLGSDALDDFDLDML" CDS complement(426..446) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 tcgatataca acatatccaa atcgaagtca tcgagcgcat cggagcccag catgtccagg 61 tcaaagtcgt ccagggcatc actccccagc atatccaagt caaaatcatc caaggcatcg 121 gaccccagca tgtccagatc gaaatcgtcc agggcgtcgc tgccgagcat gtccaggtcg 181 aaatcatcaa gggcatcgct tcccaacata tcaaggtcga aatcatccag tgcgtctgac 241 cctaacatat cgagatcgaa atcgtccaga gcatcggagc cgagcatgtc cagatcaaag 301 tcatccaatg cgtcgcttcc caacatatcc aggtcaaagt catcgagggc atcagaaccc 361 agcatgtcga gatcgaaatc gtccagcgcg tcggcgcgcc cggatccggc cggcccgctg 421 gcctccacct ttctcttctt cttggggctg tcgcctccca gctgagacag gtcgatccgt 481 gtctcgtaca ggccggtgat gctctggtgg atcagggtgg cgtccagcac ctctttggtg 541 ctggtgtacc tcttccggtc gatggtggtg tcaaagtact tgaaggcggc aggggctccc 601 agattggtca gggtaaacag gtggatgata ttctcggcct gctctctgat gggcttatcc 661 cggtgcttgt tgtaggcgga cagcactttg tccagattag cgtcggccag gatcactctc 721 ttggagaact cgctgatctg ctcgatgatc tcgtccaggt agtgcttgtg ctgttccac //