LOCUS Exported 454 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters. ACCESSION . VERSION . KEYWORDS pAC154-dual-dCas9VP160-sgExpression SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 454) AUTHORS Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R TITLE Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. JOURNAL Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. PUBMED 23979020 REFERENCE 2 (bases 1 to 454) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..454 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 18..38 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 ttccagatta cgcttcgccg aagaaaaagc gcaaggtcga agcgtccgac aagaagtaca 61 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca 121 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga 181 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga 241 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct 301 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc 361 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg 421 tggcctacca cgagaagtac cccaccatct acca //