LOCUS Exported 4278 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS dBrainbow SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4278) AUTHORS Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH TITLE Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide neural expression patterns. JOURNAL Nat Methods. 2011 Feb 6. ():. PUBMED 21297621 REFERENCE 2 (bases 1 to 4278) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4278 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 4..25 /label=pCasper-hs /note="Drosophila Hsp70 promoter, forward primer" protein_bind complement(67..100) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." protein_bind complement(122..155) /label=lox5171 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GTACACAT). lox5171 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." protein_bind complement(177..210) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." intron 350..415 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 545..565 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 837..971 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(874..893) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 928..947 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" protein_bind complement(1794..1827) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." CDS 1839..2552 /codon_start=1 /product="Aequoria victoria green fluorescent protein" /label=GFP /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" primer_bind complement(1869..1897) /label=GFP-R /note="GFP, reverse primer. Does NOT anneal to EGFP" primer_bind 2492..2512 /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" CDS 2553..2585 /codon_start=1 /product="HSV (herpes simplex virus) epitope tag" /label=HSV tag /translation="QPELAPEDPED" protein_bind complement(2605..2638) /label=lox5171 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GTACACAT). lox5171 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." CDS 2643..3350 /codon_start=1 /product="monomeric teal (cyan) fluorescent protein (Ai et al., 2006)" /label=mTFP1 /note="mammalian codon-optimized" /translation="MVSKGEELTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA VARNSTDGMDELYK" CDS 3351..3377 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 3381..3407 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 3411..3437 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" protein_bind complement(3463..3496) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS 4218..4259 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" ORIGIN 1 actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag aactctgaat 61 agatctataa cttcgtataa agtatcctat acgaagttat gaggcagcgc ggctatcgtg 121 gataacttcg tataatgtgt actatacgaa gttatctggc cacgacgggc gttcctataa 181 cttcgtataa tgtatgctat acgaagttat gccctgaatg cggccgccag tgtgatggat 241 atctgcagaa ttcgcccttc ggccgccaaa atggatcttt gtgaaggaac cttacttctg 301 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa 361 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc 421 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt 481 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac 541 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag 601 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac 661 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga tgtatagtgc 721 cttgactaga gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa 781 acctcccaca cctccctctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact 841 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 901 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 961 atgtctggat ctgcgtcgcg ccacttcaac gctcgatggg agcgtcattg gtgggcgggg 1021 taaccgtcga aatcagtgtt tacgcttcca atcgcaacaa aaaattcact gcaacactga 1081 aaagcatacg aaaacgatga agattgtacg aggaaccata aagtatttta tccacaaaga 1141 cacgtatagc agaaaagcca agttaactcg gcgataagtt gtgtacacaa gaataaaatc 1201 ggccagattc agtgttgtca gaaataagaa aaccccacta tgtttttctt tgccttttct 1261 ttctcccagc gatcattcat ttcgtggtga aagaacgggg tcattgcacg gagtttcgac 1321 tgcgggaaag cagagctgcc gttcacttcg tctataatta gcgctttcta ttttccccga 1381 ttcgggccgc tgctgcgctt ttccgcctgc tgtttgtggc aagtgtagca gcaggctgtg 1441 cacgcagtgt ggcatgcact tggctttcca ccgttggtat cgattctctg ggacgatgag 1501 tcattccttt cggggccaca gcataatcgt tgccagctca ccgaaatggt gacttcattt 1561 cttaactgcc gtcaagcatg cgattgtaca tacatacata tttatatatg tacatattta 1621 tgtgactatg gtaggtcgat ataatagcaa tcaacgcaag caaatgtgtc agtcctgctt 1681 acaggaacga ttctatttag taattttcgt tgtataaagt aattatgtat gtatgtaagc 1741 cccataaatc tgaaacaatt aggcaaaacc atgcgaggat gaactgcaag acgataactt 1801 cgtataaagt atcctatacg aagttatgcg gccgcaaaat gagtaaagga gaagaacttt 1861 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt 1921 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt 1981 gcactactgg aaaactacct gttccatggc caacacttgt cactacttta acttatggtg 2041 ttcaatgctt ttcaagatac ccagatcata tgaaacagca tgactttttc aagagtgcca 2101 tgcccgaagg ttatgtccag gaaagaacta tatttttcaa agatgacggg aactacaaga 2161 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta 2221 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac 2281 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta 2341 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa 2401 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt 2461 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg 2521 ggattacaca tggcatggat gaactataca aacagcccga gctggccccc gaggatcccg 2581 aggattgacg taagctagct cgagataact tcgtataatg tgtactatac gaagttatca 2641 aaatggtgag caagggcgag gagctcacaa tgggcgtaat caagcccgac atgaagatca 2701 agctgaagat ggagggcaac gtgaatggcc acgccttcgt gatcgagggc gagggcgagg 2761 gcaagcccta cgacggcacc aacaccatca acctggaggt gaaggaggga gcccccctgc 2821 ccttctccta cgacattctg accaccgcgt tcgcctacgg caacagggcc ttcaccaagt 2881 accccgacga catccccaac tacttcaagc agtccttccc cgagggctac tcttgggagc 2941 gcaccatgac cttcgaggac aagggcatcg tgaaggtgaa gtccgacatc tccatggagg 3001 aggactcctt catctacgag atacacctca agggcgagaa cttccccccc aacggccccg 3061 tgatgcagaa gaagaccacc ggctgggacg cctccaccga gaggatgtac gtgcgcgacg 3121 gcgtgctgaa gggcgacgtc aagcacaagc tgctgctgga gggcggcggc caccaccgcg 3181 ttgacttcaa gaccatctac agggccaaga aggcggtgaa gctgcccgac tatcactttg 3241 tggaccaccg catcgagatc ctgaaccacg acaaggacta caacaaggtg accgtttacg 3301 agagcgccgt ggcccgcaac tccaccgacg gcatggacga gctgtacaag tacccatacg 3361 atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga tatccatatg 3421 acgttccaga ttacgctggc ggataacgta agctagggta ccataacttc gtataatgta 3481 tgctatacga agttatcaaa atgataccgg tcggagtaag caaaggtgag gagagcgtga 3541 tcaaacccga gatgaagatg cgctactata tggacggcag cgtcaacggc cacgagttta 3601 ccatcgaggg cgagggcacg ggacgcccgt acgaaggaca ccaggagatg accctgcgcg 3661 tgaccatggc cgagggtggc ccgatgccct tcgccttcga tctggtgagc cacgtgttct 3721 gctacggtca ccgcgtcttc accaaatacc ccgaggaaat ccccgattac ttcaagcaag 3781 ccttcccgga gggcctgtcg tgggagcgca gcctggagtt cgaggatggc ggaagcgcca 3841 gcgtgagcgc gcacatcagc ctgcggggca acaccttcta ccataagagc aagttcaccg 3901 gcgtgaattt cccagccgat ggccccatca tgcagaacca gagtgtggat tgggagccca 3961 gcaccgagaa gatcaccgca agcgatggcg tgctgaaggg cgatgttacc atgtacctga 4021 agctggaggg aggcggcaac cacaagtgcc agatgaaaac cacctacaag gccgccaagg 4081 ggatcctgga gatgcccgga gatcactaca ttggccaccg cctggtccgc aagacagaag 4141 gaaacatcac cgagcaggtg gaggatgccg tggcgcattc gggcatggat gagctgtaca 4201 agggcgcgcc agctaatggc aagcccatcc ccaaccccct gctgggcctg gatagcacct 4261 gacgtaagct agtctaga //