LOCUS       Exported                 701 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  PT3 GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    pTHSSd_9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(88..106)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(538..557)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttgctcatc tttacctcct ctatcgcgga aattgacagg atcctctccc tatagtgagg
       61 gttattactc gaggtgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
      121 tcatgataat aatggtttct tagacgtcgg aattgccagc tggggcgccc tctggtaagg
      181 ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca
      241 ggggatcaag atctgatcaa gagacaggat gaggatcgtt tcgcgataaa tgcttcaata
      301 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      361 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      421 tgaagatcag ttgggtgcac gagtgggtta tatcgaactg gatctcaaca gcggtaagat
      481 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      541 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      601 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      661 catgacagta agagaattat gcagtgctgc cataaccatg a
//
