LOCUS       Exported                 840 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  MESA target chain with CD4 ectodomain, no linker domain, G cleavage 
            sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    CD4_LD 0_CS G_tTA-BFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     279..298
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             complement(372..392)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccttgttggc ataaaaaggc taattgattt tcgagagttt catactgttt ttctgtaggc
       61 cgtgtaccta aatgtacttt tgctccatcg cgatgactta gtaaagcaca tctaaaactt
      121 ttagcgttat tacgtaaaaa atcttgccag ctttcccctt ctaaagggca aaagtgagta
      181 tggtgcctat ctaacatctc aatggctaag gcgtcgagca aagcccgctt attttttaca
      241 tgccaataca atgtaggctg ctctacacct agcttctggg cgagtttacg ggttgttaaa
      301 ccttcgattc cgacctcatt aagcagctct aatgcgctgt taatcacttt acttttatct
      361 aatctagaca taccctgaaa atacaggttt tcaacacaaa gagccactgt cactagcaag
      421 ccataacaaa acaggactcc agcaaccacg accagtgccc aaaaaagctt ccctctggat
      481 aaaacctgga tcctggagtc catcttgacc ttatcacctt cactcagtag acactgccac
      541 agccctgtct caggggccac cacttgaact actttctgct cctcagagac cctggcctcc
      601 tggttctcct gcttcagggt cagtctcatc ttgggagagg taggtcccat cacctcacag
      661 gtcaaagtat tgttgagctg agccactttc atcaccacca ggttcacttc ctgatgcagt
      721 gtccctttgt ccagagtcag agtcaggttg ccagaaccag caaactgaag cgagacctgg
      781 ggtatcttga gggtgagtgg gagcgtttcc ttcagctgga gcttgaggtc tttggtggac
//
