LOCUS       Exported                 645 bp ds-DNA     linear   SYN 11-JUN-2021
DEFINITION  MESA target chain with dTomato ectodomain, no linker domain, M 
            cleavage sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    dTomato_LD 0_CS M_tTA-BFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(305..323)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     573..592
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gccatcatgt gccgagccat ctctcttagg
      121 cgcttgctgc tgctgctgct gcagctgtca caactcctag ctgtcactca aggggtgagc
      181 aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg
      241 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
      301 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
      361 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac
      421 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
      481 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
      541 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
      601 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctg
//
