LOCUS       Exported                 654 bp ds-DNA     linear   SYN 11-JUN-2021
DEFINITION  MESA target chain with dTomato ectodomain, no linker domain, M 
            cleavage sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    dTomato_LD 0_CS M_tTA-BFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             175..405
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYG"
     primer_bind     complement(484..505)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 caacttaaat gtgaaagtgg gtccgcgtac agccgcgcgc gtacgaaaaa caattacggg
       61 tctaccatcg agggcctgct cgatctcccg gacgacgacg cccccgaaga ggcggggctg
      121 gcggctccgc gcctgtcctt tctccccgcg ggacacacgc gcagactgtc gacggccccc
      181 ccgaccgatg tcagcctggg ggacgagctc cacttagacg gcgaggacgt ggcgatggcg
      241 catgccgacg cgctagacga tttcgatctg gacatgttgg gggacgggga ttccccgggt
      301 ccgggattta ccccccacga ctccgccccc tacggcgctc tggatatggc cgacttcgag
      361 tttgagcaga tgtttaccga tgcccttgga attgacgagt acggtggagg taccggcgga
      421 ggctccggtg gtggctctat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      481 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgag gggcgagggc
      541 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      601 cccgtgccct ggcccaccct cgtgaccacc ctgagccacg gcgtgcagtg cttc
//
