LOCUS       Exported                 733 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  MESA target chain with Rapamycin FRB ectodomain, no linker domain, G
            cleavage sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    FRB_LD 0_CS G_tTA-BFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(56..286)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYG"
ORIGIN
        1 gctcctcgcc cttgctcacc atagagccac caccggagcc tccgccggta cctccaccgt
       61 actcgtcaat tccaagggca tcggtaaaca tctgctcaaa ctcgaagtcg gccatatcca
      121 gagcgccgta gggggcggag tcgtgggggg taaatcccgg acccggggaa tccccgtccc
      181 ccaacatgtc cagatcgaaa tcgtctagcg cgtcggcatg cgccatcgcc acgtcctcgc
      241 cgtctaagtg gagctcgtcc cccaggctga catcggtcgg gggggccgtc gacagtctgc
      301 gcgtgtgtcc cgcggggaga aaggacaggc gcggagccgc cagccccgcc tcttcggggg
      361 cgtcgtcgtc cgggagatcg agcaggccct cgatggtaga cccgtaattg tttttcgtac
      421 gcgcgcggct gtacgcggac ccactttcac atttaagttg tttttctaat ccgcatatga
      481 tcaattcaag gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag
      541 cttgtcgtaa taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga
      601 cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg
      661 catataatgc attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga
      721 gagtttcata ctg
//
