LOCUS Exported 10526 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION piggyBac vector with tet inducible mcMyc. ACCESSION . VERSION . KEYWORDS PB-TET-mcMyc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10526) AUTHORS Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hamalainen R, Cowling R, Wang W, Liu P, Gertsenstein M, Kaji K, Sung HK, Nagy A TITLE piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. JOURNAL Nature. 2009 Mar 1. ():. PUBMED 19252478 REFERENCE 2 (bases 1 to 10526) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10526 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(3..458) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(140..161) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 352..371 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 602..619 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 603..619 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 626..645 /label=T7 /note="T7 promoter, forward primer" promoter 626..644 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" repeat_region 711..773 /label=piggyBac right (3') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" protein_bind 1145..1163 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1182..1200 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1219..1237 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1256..1274 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1293..1311 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1330..1348 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1367..1385 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1404..1422 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 1444..1468 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 1548..1572 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(2929..2953) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(3171..3188) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3320..3339 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 3551..3571 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 3581..6622 /codon_start=1 /gene="lacZ" /product="beta-galactosidase" /label=lacZ /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD SWSPSVSAEFQLSAGRYHYQLVWCQ" CDS 6647..7450 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6710..6729) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7320..7339 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(7482..7499) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7488..7712 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 8151..8185 /label=piggyBac left (5') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" promoter complement(8335..8353) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(8374..8390) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8374..8390) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8387..8409) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8398..8414 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8422..8452) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8467..8488 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8605..8622) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8776..9364) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8856..8875) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9535..10395) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10158..10177 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10396..10500) /gene="bla" /label=AmpR promoter ORIGIN 1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct 481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg 661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag 721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta 781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt 841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa 901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct 961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt 1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac 1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca 1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga 1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct 1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac 1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat 1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag 1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca 1561 aaaaagcagg ctccgcggcc gcccccttca ccatgcccct caacgtgaac ttcaccaaca 1621 ggaactatga cctcgactac gactccgtac agccctattt catctgcgac gaggaagaga 1681 atttctatca ccagcaacag cagagcgagc tgcagccgcc cgcgcccagt gaggatatct 1741 ggaagaaatt cgagctgctt cccaccccgc ccctgtcccc gagccgccgc tccgggctct 1801 gctctccatc ctatgttgcg gtcgctacgt ccttctcccc aagggaagac gatgacggcg 1861 gcggtggcaa cttctccacc gccgatcagc tggagatgat gaccgagtta cttggaggag 1921 acatggtgaa ccagagcttc atctgcgatc ctgacgacga gaccttcatc aagaacatca 1981 tcatccagga ctgtatgtgg agcggtttct cagccgctgc caagctggtc tcggagaagc 2041 tggcctccta ccaggctgcg cgcaaagaca gcaccagcct gagccccgcc cgcgggcaca 2101 gcgtctgctc cacctccagc ctgtacctgc aggacctcac cgccgccgcg tccgagtgca 2161 ttgacccctc agtggtcttt ccctacccgc tcaacgacag cagctcgccc aaatcctgta 2221 cctcgtccga ttccacggcc ttctctcctt cctcggactc gctgctgtcc tccgagtcct 2281 ccccacgggc cagccctgag cccctagtgc tgcatgagga gacaccgccc accaccagca 2341 gcgactctga agaagagcaa gaagatgagg aagaaattga tgtggtgtct gtggagaaga 2401 ggcaaacccc tgccaagagg tcggagtcgg gctcatctcc atcccgaggc cacagcaaac 2461 ctccgcacag cccactggtc ctcaagaggt gccacgtctc cactcaccag cacaactacg 2521 ccgcaccccc ctccacaagg aaggactatc cagctgccaa gagggccaag ttggacagtg 2581 gcagggtcct gaagcagatc agcaacaacc gcaagtgctc cagccccagg tcctcagaca 2641 cggaggaaaa cgacaagagg cggacacaca acgtcttgga acgtcagagg aggaacgagc 2701 tgaagcgcag cttttttgcc ctgcgtgacc agatccctga attggaaaac aacgaaaagg 2761 cccccaaggt agtgatcctc aaaaaagcca ccgcctacat cctgtccatt caagcagacg 2821 agcacaagct cacctctgaa aaggacttat tgaggaaacg acgagaacag ttgaaacaca 2881 aactcgaaca gcttcgaaac tctggtgcat aaaagggtgg gcgcgccgac ccagctttct 2941 tgtacaaagt ggtgatggcc gctctagcta gtcgataagc taattccgcc cccccccccc 3001 cccccccctc accctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc 3061 ggtgagcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg 3121 cccgaaacct ggccctgtct tcttgacgac gattcctagg ggtctttccc ctctcgccaa 3181 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag 3241 acaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacatggat 3301 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc 3361 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg 3421 tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttggtt 3481 tgaaaaacac gatgataata tggccacaac catggaacag ggggatcagc ttaccatggc 3541 caagatccct cctaagaaga agcgcaaagt cgaggatccc gtcgttttac aacgtcgtga 3601 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 3661 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 3721 tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga 3781 gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta 3841 cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc cgtttgttcc 3901 cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca 3961 ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa 4021 cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc 4081 atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgttgga gtgacggcag 4141 ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct 4201 gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt 4261 cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg 4321 ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg 4381 cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt 4441 cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact 4501 gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt 4561 gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa 4621 ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga 4681 tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca 4741 tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat 4801 tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc 4861 gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat 4921 ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat 4981 caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc 5041 caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt 5101 gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct 5161 ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc 5221 gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct 5281 gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac 5341 gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc 5401 agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac 5461 catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat 5521 ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca 5581 aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg 5641 gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag 5701 cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca 5761 cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg 5821 ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca 5881 actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt 5941 aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca 6001 ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct 6061 gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac 6121 ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga 6181 tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt 6241 aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt 6301 tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa 6361 cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt 6421 ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct 6481 gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg 6541 cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg gtcgctacca 6601 ttaccagttg gtctggtgtc aggggatccc ccgggctgca gccaatatgg gatcggccat 6661 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 6721 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 6781 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 6841 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 6901 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 6961 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 7021 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 7081 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 7141 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 7201 tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 7261 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 7321 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 7381 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 7441 gttcttctga ggggatcaat tctctagggc tcgctgatca gcctcgactg tgccttctag 7501 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 7561 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 7621 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 7681 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg 7741 ctcgatcctc tagagtcgag ggctgcagga attcgatatc aagctctagc caacaagctc 7801 gtcatcgctt tgcagaagag cagagaggat atgctcatcg tctaaagaac tacccatttt 7861 attatatatt agtcacgata tctataacaa gaaaatatat atataataag ttatcacgta 7921 agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa 7981 agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta 8041 ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca 8101 cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa 8161 ttttacgcag actatctttc tagggttaaa aaagatttgc gctttactcg acctaaactt 8221 taaacacgtc atagaatctt cgtttgacaa aaaccacatt gtggccaagc tgtgtgacgc 8281 gacgcgcgct aaagaatggc aaaccaagtc gcgcgaggta cccagctttt gttcccttta 8341 gtgagggtta attccgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 8401 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 8461 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 8521 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 8581 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 8641 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 8701 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 8761 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 8821 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 8881 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 8941 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 9001 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 9061 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 9121 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 9181 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 9241 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 9301 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 9361 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 9421 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 9481 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 9541 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 9601 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 9661 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 9721 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 9781 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 9841 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 9901 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 9961 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 10021 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 10081 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 10141 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 10201 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 10261 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 10321 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 10381 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 10441 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 10501 cacatttccc cgaaaagtgc cacctg //