LOCUS Exported 10649 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION piggyBac vector with tet inducible mKLF4. ACCESSION . VERSION . KEYWORDS PB-TET-mKLF4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10649) AUTHORS Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hamalainen R, Cowling R, Wang W, Liu P, Gertsenstein M, Kaji K, Sung HK, Nagy A TITLE piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. JOURNAL Nature. 2009 Mar 1. ():. PUBMED 19252478 REFERENCE 2 (bases 1 to 10649) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10649 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(3..458) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(140..161) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 352..371 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 602..619 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 603..619 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 626..645 /label=T7 /note="T7 promoter, forward primer" promoter 626..644 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" repeat_region 711..773 /label=piggyBac right (3') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" protein_bind 1145..1163 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1182..1200 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1219..1237 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1256..1274 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1293..1311 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1330..1348 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1367..1385 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1404..1422 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 1444..1468 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 1548..1572 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(3052..3076) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(3294..3311) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3443..3462 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 3674..3694 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 3704..6745 /codon_start=1 /gene="lacZ" /product="beta-galactosidase" /label=lacZ /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD SWSPSVSAEFQLSAGRYHYQLVWCQ" CDS 6770..7573 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6833..6852) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7443..7462 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(7605..7622) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7611..7835 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 8274..8308 /label=piggyBac left (5') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" promoter complement(8458..8476) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(8497..8513) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8497..8513) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8510..8532) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8521..8537 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8545..8575) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8590..8611 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8728..8745) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8899..9487) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8979..8998) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9658..10518) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10281..10300 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10519..10623) /gene="bla" /label=AmpR promoter ORIGIN 1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct 481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg 661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag 721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta 781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt 841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa 901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct 961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt 1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac 1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca 1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga 1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct 1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac 1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat 1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag 1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca 1561 aaaaagcagg ctccgcggcc gcccccttca ccatggctgt cagcgacgct ctgctcccgt 1621 ccttctccac gttcgcgtcc ggcccggcgg gaagggagaa gacactgcgt ccagcaggtg 1681 ccccgactaa ccgttggcgt gaggaactct ctcacatgaa gcgacttccc ccacttcccg 1741 gccgccccta cgacctggcg gcgacggtgg ccacagacct ggagagtggc ggagctggtg 1801 cagcttgcag cagtaacaac ccggccctcc tagcccggag ggagaccgag gagttcaacg 1861 acctcctgga cctagacttt atcctttcca actcgctaac ccaccaggaa tcggtggccg 1921 ccaccgtgac cacctcggcg tcagcttcat cctcgtcttc cccggcgagc agcggccctg 1981 ccagcgcgcc ctccacctgc agcttcagct atccgatccg ggccgggggt gacccgggcg 2041 tggctgccag caacacaggt ggagggctcc tctacagccg agaatctgcg ccacctccca 2101 cggccccctt caacctggcg gacatcaatg acgtgagccc ctcgggcggc ttcgtggctg 2161 agctcctgcg gccggagttg gacccagtat acattccgcc acagcagcct cagccgccag 2221 gtggcgggct gatgggcaag tttgtgctga aggcgtctct gaccacccct ggcagcgagt 2281 acagcagccc ttcggtcatc agtgttagca aaggaagccc agacggcagc caccccgtgg 2341 tagtggcgcc ctacagcggt ggcccgccgc gcatgtgccc caagattaag caagaggcgg 2401 tcccgtcctg cacggtcagc cggtccctag aggcccattt gagcgctgga ccccagctca 2461 gcaacggcca ccggcccaac acacacgact tccccctggg gcggcagctc cccaccagga 2521 ctacccctac actgagtccc gaggaactgc tgaacagcag ggactgtcac cctggcctgc 2581 ctcttccccc aggattccat ccccatccgg ggcccaacta ccctcctttc ctgccagacc 2641 agatgcagtc acaagtcccc tctctccatt atcaagagct catgccaccg ggttcctgcc 2701 tgccagagga gcccaagcca aagaggggaa gaaggtcgtg gccccggaaa agaacagcca 2761 cccacacttg tgactatgca ggctgtggca aaacctatac caagagttct catctcaagg 2821 cacacctgcg aactcacaca ggcgagaaac cttaccactg tgactgggac ggctgtgggt 2881 ggaaattcgc ccgctccgat gaactgacca ggcactaccg caaacacaca gggcaccggc 2941 cctttcagtg ccagaagtgc gacagggcct tttccaggtc ggaccacctt gccttacaca 3001 tgaagaggca cttttaaatc ccacgtagtg gatgtaaggg tgggcgcgcc gacccagctt 3061 tcttgtacaa agtggtgatg gccgctctag ctagtcgata agctaattcc gccccccccc 3121 cccccccccc ctcaccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag 3181 gccggtgagc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga 3241 gggcccgaaa cctggccctg tcttcttgac gacgattcct aggggtcttt cccctctcgc 3301 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg 3361 aagacaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacatg 3421 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga 3481 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac 3541 atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttg 3601 gtttgaaaaa cacgatgata atatggccac aaccatggaa cagggggatc agcttaccat 3661 ggccaagatc cctcctaaga agaagcgcaa agtcgaggat cccgtcgttt tacaacgtcg 3721 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 3781 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 3841 gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct 3901 ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg 3961 ttacgatgcg cccatctaca ccaacgtaac ctatcccatt acggtcaatc cgccgtttgt 4021 tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct 4081 acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc atctgtggtg 4141 caacgggcgc tgggtcggtt acggccagga cagtcgtttg ccgtctgaat ttgacctgag 4201 cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgtt ggagtgacgg 4261 cagttatctg gaagatcagg atatgtggcg gatgagcggc attttccgtg acgtctcgtt 4321 gctgcataaa ccgactacac aaatcagcga tttccatgtt gccactcgct ttaatgatga 4381 tttcagccgc gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc gtgactacct 4441 acgggtaaca gtttctttat ggcagggtga aacgcaggtc gccagcggca ccgcgccttt 4501 cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa 4561 cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg cggtggttga 4621 actgcacacc gccgacggca cgctgattga agcagaagcc tgcgatgtcg gtttccgcga 4681 ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt 4741 taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca 4801 ggatatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc attatccgaa 4861 ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa 4921 tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc gctggctacc 4981 ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat 5041 catctggtcg ctggggaatg aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg 5101 gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag ccgacaccac 5161 ggccaccgat attatttgcc cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc 5221 tgtgccgaaa tggtccatca aaaaatggct ttcgctacct ggagagacgc gcccgctgat 5281 cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta aatactggca 5341 ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc tgggactggg tggatcagtc 5401 gctgattaaa tatgatgaaa acggcaaccc gtggtcggct tacggcggtg attttggcga 5461 tacgccgaac gatcgccagt tctgtatgaa cggtctggtc tttgccgacc gcacgccgca 5521 tccagcgctg acggaagcaa aacaccagca gcagtttttc cagttccgtt tatccgggca 5581 aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc gataacgagc tcctgcactg 5641 gatggtggcg ctggatggta agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc 5701 acaaggtaaa cagttgattg aactgcctga actaccgcag ccggagagcg ccgggcaact 5761 ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat 5821 cagcgcctgg cagcagtggc gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc 5881 ccacgccatc ccgcatctga ccaccagcga aatggatttt tgcatcgagc tgggtaataa 5941 gcgttggcaa tttaaccgcc agtcaggctt tctttcacag atgtggattg gcgataaaaa 6001 acaactgctg acgccgctgc gcgatcagtt cacccgtgca ccgctggata acgacattgg 6061 cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg 6121 ccattaccag gccgaagcag cgttgttgca gtgcacggca gatacacttg ctgatgcggt 6181 gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta tcagccggaa 6241 aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg aagtggcgag 6301 cgatacaccg catccggcgc ggattggcct gaactgccag ctggcgcagg tagcagagcg 6361 ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta ctgccgcctg 6421 ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct tcccgagcga 6481 aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga 6541 cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca gccatcgcca 6601 tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata tggggattgg 6661 tggcgacgac tcctggagcc cgtcagtatc ggcggaattc cagctgagcg ccggtcgcta 6721 ccattaccag ttggtctggt gtcaggggat cccccgggct gcagccaata tgggatcggc 6781 cattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 6841 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 6901 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 6961 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 7021 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 7081 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 7141 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 7201 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 7261 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 7321 cgatgatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 7381 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 7441 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 7501 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 7561 cgagttcttc tgaggggatc aattctctag ggctcgctga tcagcctcga ctgtgccttc 7621 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 7681 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 7741 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 7801 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 7861 gggctcgatc ctctagagtc gagggctgca ggaattcgat atcaagctct agccaacaag 7921 ctcgtcatcg ctttgcagaa gagcagagag gatatgctca tcgtctaaag aactacccat 7981 tttattatat attagtcacg atatctataa caagaaaata tatatataat aagttatcac 8041 gtaagtagaa catgaaataa caatataatt atcgtatgag ttaaatctta aaagtcacgt 8101 aaaagataat catgcgtcat tttgactcac gcggtcgtta tagttcaaaa tcagtgacac 8161 ttaccgcatt gacaagcacg cctcacggga gctccaagcg gcgactgaga tgtcctaaat 8221 gcacagcgac ggattcgcgc tatttagaaa gagagagcaa tatttcaaga atgcatgcgt 8281 caattttacg cagactatct ttctagggtt aaaaaagatt tgcgctttac tcgacctaaa 8341 ctttaaacac gtcatagaat cttcgtttga caaaaaccac attgtggcca agctgtgtga 8401 cgcgacgcgc gctaaagaat ggcaaaccaa gtcgcgcgag gtacccagct tttgttccct 8461 ttagtgaggg ttaattccga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 8521 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 8581 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 8641 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 8701 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 8761 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 8821 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 8881 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 8941 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 9001 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 9061 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 9121 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 9181 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 9241 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 9301 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 9361 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 9421 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 9481 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 9541 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 9601 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 9661 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 9721 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 9781 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 9841 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 9901 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 9961 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 10021 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 10081 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 10141 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 10201 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 10261 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 10321 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 10381 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 10441 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 10501 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 10561 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 10621 gcgcacattt ccccgaaaag tgccacctg //