LOCUS Exported 10398 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION piggyBac vector with tet inducible mOct4. ACCESSION . VERSION . KEYWORDS PB-TET-mOct4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10398) AUTHORS Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hamalainen R, Cowling R, Wang W, Liu P, Gertsenstein M, Kaji K, Sung HK, Nagy A TITLE piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. JOURNAL Nature. 2009 Mar 1. ():. PUBMED 19252478 REFERENCE 2 (bases 1 to 10398) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10398 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(3..458) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(140..161) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 352..371 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 602..619 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 603..619 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 626..645 /label=T7 /note="T7 promoter, forward primer" promoter 626..644 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" repeat_region 711..773 /label=piggyBac right (3') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" protein_bind 1145..1163 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1182..1200 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1219..1237 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1256..1274 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1293..1311 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1330..1348 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1367..1385 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1404..1422 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 1444..1468 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 1548..1572 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(2801..2825) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(3043..3060) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3192..3211 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 3423..3443 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 3453..6494 /codon_start=1 /gene="lacZ" /product="beta-galactosidase" /label=lacZ /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD SWSPSVSAEFQLSAGRYHYQLVWCQ" CDS 6519..7322 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6582..6601) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 7192..7211 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(7354..7371) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7360..7584 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 8023..8057 /label=piggyBac left (5') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" promoter complement(8207..8225) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(8246..8262) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8246..8262) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8259..8281) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8270..8286 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8294..8324) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8339..8360 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8477..8494) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8648..9236) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8728..8747) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9407..10267) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10030..10049 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10268..10372) /gene="bla" /label=AmpR promoter ORIGIN 1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct 481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg 661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag 721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta 781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt 841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa 901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct 961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt 1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac 1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca 1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga 1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct 1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac 1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat 1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag 1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca 1561 aaaaagcagg ctccaccttc cccatggctg gacacctggc ttcagacttc gccttctcac 1621 ccccaccagg tgggggtgat gggtcagcag ggctggagcc gggctgggtg gattctcgaa 1681 cctggctaag cttccaaggg cctccaggtg ggcctggaat cggaccaggc tcagaggtat 1741 tggggatctc cccatgtccg cccgcatacg agttctgcgg agggatggca tactgtggac 1801 ctcaggttgg actgggccta gtcccccaag ttggcgtgga gactttgcag cctgagggcc 1861 aggcaggagc acgagtggaa agcaactcag agggaacctc ctctgagccc tgtgccgacc 1921 gccccaatgc cgtgaagttg gagaaggtgg aaccaactcc cgaggagtcc caggacatga 1981 aagccctgca gaaggagcta gaacagtttg ccaagctgct gaagcagaag aggatcacct 2041 tggggtacac ccaggccgac gtggggctca ccctgggcgt tctctttgga aaggtgttca 2101 gccagaccac catctgtcgc ttcgaggcct tgcagctcag ccttaagaac atgtgtaagc 2161 tgcggcccct gctggagaag tgggtggagg aagccgacaa caatgagaac cttcaggaga 2221 tatgcaaatc ggagaccctg gtgcaggccc ggaagagaaa gcgaactagc attgagaacc 2281 gtgtgaggtg gagtctggag accatgtttc tgaagtgccc gaagccctcc ctacagcaga 2341 tcactcacat cgccaatcag cttgggctag agaaggatgt ggttcgagta tggttctgta 2401 accggcgcca gaagggcaaa agatcaagta ttgagtattc ccaacgagaa gagtatgagg 2461 ctacagggac acctttccca gggggggctg tatcctttcc tctgccccca ggtccccact 2521 ttggcacccc aggctatgga agcccccact tcaccacact ctactcagtc ccttttcctg 2581 agggcgaggc ctttccctct gttcccgtca ctgctctggg ctctcccatg cattcaaact 2641 gaggcaccag ccctccctgg ggatgctgtg agccaaggca agggaggtag acaagagaac 2701 ctggagcttt ggggttaaat tcttttactg aggagggatt aaaagcacaa caggggtggg 2761 gggtgggatg gggaaagaag ctcagtgatg ctgttgatca acccagcttt cttgtacaaa 2821 gtggtgatgg ccgctctagc tagtcgataa gctaattccg cccccccccc cccccccccc 2881 tcaccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgagcg 2941 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccgaaac 3001 ctggccctgt cttcttgacg acgattccta ggggtctttc ccctctcgcc aaaggaatgc 3061 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaacaac 3121 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacatgg atagttgtgg 3181 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg 3241 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt 3301 cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttgg tttgaaaaac 3361 acgatgataa tatggccaca accatggaac agggggatca gcttaccatg gccaagatcc 3421 ctcctaagaa gaagcgcaaa gtcgaggatc ccgtcgtttt acaacgtcgt gactgggaaa 3481 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 3541 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 3601 ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc 3661 ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc 3721 ccatctacac caacgtaacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga 3781 atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc 3841 agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct 3901 gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac 3961 gcgccggaga aaaccgcctc gcggtgatgg tgctgcgttg gagtgacggc agttatctgg 4021 aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac 4081 cgactacaca aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg 4141 ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag 4201 tttctttatg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa 4261 ttatcgatga gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc 4321 cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg 4381 ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg 4441 aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg 4501 agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc 4561 tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt 4621 ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc 4681 acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg 4741 aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc 4801 tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg 4861 tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata 4921 ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat 4981 ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat 5041 acgcccacgc gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc 5101 agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat 5161 atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg 5221 atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga 5281 cggaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag 5341 tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc 5401 tggatggtaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac 5461 agttgattga actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag 5521 tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc 5581 agcagtggcg tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc 5641 cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat 5701 ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga 5761 cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag 5821 cgacccgcat tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg 5881 ccgaagcagc gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga 5941 ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga 6001 ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc 6061 atccggcgcg gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc 6121 tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct 6181 gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc 6241 gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca 6301 acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg 6361 cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact 6421 cctggagccc gtcagtatcg gcggaattcc agctgagcgc cggtcgctac cattaccagt 6481 tggtctggtg tcaggggatc ccccgggctg cagccaatat gggatcggcc attgaacaag 6541 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 6601 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 6661 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 6721 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 6781 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 6841 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 6901 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 6961 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 7021 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gatgatctcg 7081 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 7141 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 7201 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 7261 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 7321 gaggggatca attctctagg gctcgctgat cagcctcgac tgtgccttct agttgccagc 7381 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 7441 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 7501 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 7561 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatcc 7621 tctagagtcg agggctgcag gaattcgata tcaagctcta gccaacaagc tcgtcatcgc 7681 tttgcagaag agcagagagg atatgctcat cgtctaaaga actacccatt ttattatata 7741 ttagtcacga tatctataac aagaaaatat atatataata agttatcacg taagtagaac 7801 atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc 7861 atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg 7921 acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg 7981 gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc 8041 agactatctt tctagggtta aaaaagattt gcgctttact cgacctaaac tttaaacacg 8101 tcatagaatc ttcgtttgac aaaaaccaca ttgtggccaa gctgtgtgac gcgacgcgcg 8161 ctaaagaatg gcaaaccaag tcgcgcgagg tacccagctt ttgttccctt tagtgagggt 8221 taattccgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 8281 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 8341 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 8401 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 8461 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 8521 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 8581 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 8641 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 8701 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 8761 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 8821 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 8881 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 8941 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 9001 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 9061 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 9121 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 9181 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 9241 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 9301 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 9361 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 9421 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 9481 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 9541 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 9601 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 9661 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 9721 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 9781 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 9841 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 9901 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 9961 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 10021 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 10081 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 10141 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 10201 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 10261 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 10321 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 10381 cccgaaaagt gccacctg //