LOCUS       Exported               15415 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  used in the derivation of human iPS cells using non-integrating 
            episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28.
ACCESSION   .
VERSION     .
KEYWORDS    pEP4 E02S EN2L
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15415)
  AUTHORS   Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
  TITLE     Human Induced Pluripotent Stem Cells Free of Vector and Transgene 
            Sequences
  JOURNAL   Science. 2009 Mar 26.
  PUBMED    19325077
REFERENCE   2  (bases 1 to 15415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15415
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        13..1175
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          244..1166
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_feature    2385..2958
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2408..2960
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     polyA_signal    3977..4111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4641..5803
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4872..5794
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_feature    6847..7420
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6870..7422
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     polyA_signal    8116..8250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8981..10770
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(11072..12997)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(13436..13454)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13522..13626
                     /gene="bla"
                     /label=AmpR promoter
     CDS             13627..14487
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13845..13864)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14658..15246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15147..15166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagcttcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
       61 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
      121 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
      181 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
      241 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
      301 gtgccttgaa ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca
      361 ggggcgggcc ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      421 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
      481 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
      541 atagtcttgt aaatgcgggc caggatctgc acactggtat ttcggttttt gggcccgcgg
      601 ccggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
      661 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
      721 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
      781 cgtgagcgga aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc
      841 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
      901 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
      961 tctggagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1021 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1081 cgttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1141 ttcaaagttt ttttcttcca tttcaggtgt cgtgaacacg tggtcgcggc caattctgca
     1201 gtcgagctca agcttcgaac tagtgattat ggcgggacac ctggcttcgg atttcgcctt
     1261 ctcgccccct ccaggtggtg gaggtgatgg gccagggggg ccggagccgg gctgggttga
     1321 tcctcggacc tggctaagct tccaaggccc tcctggaggg ccaggaatcg ggccgggggt
     1381 tgggccaggc tctgaggtgt gggggattcc cccatgcccc ccgccgtatg agttctgtgg
     1441 ggggatggcg tactgtgggc cccaggttgg agtggggcta gtgccccaag gcggcttgga
     1501 gacctctcag cctgagggtg aagcangagt cggggtggag agcaactccg atggggcctc
     1561 cccggagccc tgcaccgtca cccctggtgc cgtgaagctg gagaaggaga agctggagca
     1621 aaacccggag gagtcccagg acatcaaagc tctgcagaaa gaactcgagc aatttgccaa
     1681 gctcctgaag cagaagagga tcaccctggg atatacacag gccgatgtgg ggctcaccct
     1741 gggggttcta tttgggaagg tattcagcca aacgaccatc tgccgctttg aggctctgca
     1801 gcttagcttc aagaacatgt gtaagctgcg gcccttgctg cagaagtggg tggaggaagc
     1861 tgacaacaat gaaaatcttc aggagatatg caaagcagaa accctcgtgc aggcccgaaa
     1921 gagaaagcga accagtatcg agaaccgagt gagaggcaac ctggagaatt tgttcctgca
     1981 gtgcccgaaa cccacactgc agcagatcag ccacatcgcc cagcagcttg ggctcgagaa
     2041 ggatgtggtc cgagtgtggt tctgtaaccg gcgccagaag ggcaagcgat caagcagcga
     2101 ctatgcacaa cgagaggatt ttgaggctgc tgggtctcct ttctcagggg gaccagtgtc
     2161 ctttcctctg gccccagggc cccattttgg taccccaggc tatgggagcc ctcacttcac
     2221 tgcactgtac tcctcggtcc ctttccctga gggggaagcc tttccccctg tctccgtcac
     2281 cactctgggc tctcccatgc attcaaactg aggtgcctgc cctctagttt acgcgtgggg
     2341 gcggccggcc gctttctcga ggccatcgat gtttaaacga tccgcccctc tccctccccc
     2401 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     2461 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     2521 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     2581 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     2641 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     2701 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     2761 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     2821 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt
     2881 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     2941 tgaaaaacac gatgataata tggccacacc gcgaattaat tcgattccgc atgtacaaca
     3001 tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc ggcggcggca
     3061 actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc gtcaagcggc
     3121 ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc caggagaacc
     3181 ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa cttttgtcgg
     3241 agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagcgctg cacatgaagg
     3301 agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg aagaaggata
     3361 agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg agcggggtcg
     3421 gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac gcgcacatga
     3481 acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac ccgcagcacc
     3541 cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac gacgtgagcg
     3601 ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg cccacctaca
     3661 gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg ggttcggtgg
     3721 tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac tccagggcgc
     3781 cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc gccgaggtgc
     3841 cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc ggcccggtgc
     3901 ccggcacggc cattaacggc acactgcccc tctcacacat gtgagggccg gaaatcacta
     3961 gctagttcga aggatcgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4021 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4081 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     4141 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     4201 tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     4261 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     4321 gcccgtcagg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgaccaat
     4381 tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc
     4441 gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat tagccatatt
     4501 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
     4561 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg
     4621 attattgact agcttcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     4681 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     4741 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     4801 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     4861 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     4921 gcccttgcgt gccttgaatt acttccacct ggctccagta cgtgattctt gatcccgagc
     4981 tggagccagg ggcgggcctt gcgctttagg agccccttcg cctcgtgctt gagttgaggc
     5041 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     5101 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     5161 ctggcaagat agtcttgtaa atgcgggcca ggatctgcac actggtattt cggtttttgg
     5221 gcccgcggcc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     5281 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     5341 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     5401 accagttgcg tgagcggaaa gatggccgct tcccggccct gctccagggg gctcaaaatg
     5461 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     5521 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     5581 cgattagttc tggagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     5641 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     5701 gtaattctcg ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     5761 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaacacgtg gtcgcggcca
     5821 attctgcagt cgagctcaag cttcgaacta gggccgcgac tctagtgatt catgagtgtg
     5881 gatccagctt gtccccaaag cttgccttgc tttgaagcat ccgactgtaa agaatcttca
     5941 cctatgcctg tgatttgtgg gcctgaagaa aactatccat ccttgcaaat gtcttctgct
     6001 gagatgcctc acacggagac tgtctctcct cttccttcct ccatggatct gcttattcag
     6061 gacagccctg attcttccac cagtcccaaa ggcaaacaac ccacttctgc agagaagagt
     6121 gtcgcaaaaa aggaagacaa ggtcccggtc aagaaacaga agaccagaac tgtgttctct
     6181 tccacccagc tgtgtgtact caatgataga tttcagagac agaaatacct cagcctccag
     6241 cagatgcaag aactctccaa catcctgaac ctcagctaca aacaggtgaa gacctggttc
     6301 cagaaccaga gaatgaaatc taagaggtgg cagaaaaaca actggccgaa gaatagcaat
     6361 ggtgtgacgc agaaggcctc agcacctacc taccccagcc tttactcttc ctaccaccag
     6421 ggatgcctgg tgaacccgac tgggaacctt ccaatgtgga gcaaccagac ctggaacaat
     6481 tcaacctgga gcaaccagac ccagaacatc cagtcctgga gcaaccactc ctggaacact
     6541 cagacctggt gcacccaatc ctggaacaat caggcctgga acagtccctt ctataactgt
     6601 ggagaggaat ctctgcagtc ctgcatgcag ttccagccaa attctcctgc cagtgacttg
     6661 gaggctgcct tggaagctgc tggggaaggc cttaatgtaa tacagcagac cactaggtat
     6721 tttagtactc cacaaaccat ggatttattc ctaaactact ccatgaacat gcaacctgaa
     6781 gacgtgtgaa gatgaatcga attcccgcgg ccgctttctc gaggccatcg atgtttaaac
     6841 gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     6901 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     6961 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     7021 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     7081 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     7141 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     7201 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     7261 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     7321 tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     7381 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca ccgcgatcgc
     7441 gaattcgatt atcgccatgg gctccgtgtc caaccagcag tttgcaggtg gctgcgccaa
     7501 ggcggcagaa gaggcgcccg aggaggcgcc ggaggacgcg gcccgggcgg cggacgagcc
     7561 tcagctgctg cacggtgcgg gcatctgtaa gtggttcaac gtgcgcatgg ggttcggctt
     7621 cctgtccatg accgcccgcg ccggggtcgc gctcgacccc ccagtggatg tctttgtgca
     7681 ccagagtaag ctgcacatgg aagggttccg gagcttgaag gagggtgagg cagtggagtt
     7741 cacctttaag aagtcagcca agggtctgga atccatccgt gtcaccggac ctggtggagt
     7801 attctgtatt gggagtgaga ggcggccaaa aggaaagagc atgcagaagc gcagatcaaa
     7861 aggagacagg tgctacaact gtggaggtct agatcatcat gccaaggaat gcaagctgcc
     7921 accccagccc aagaagtgcc acttctgcca gagcatcagc catatggtag cctcatgtcc
     7981 gctgaaggcc cagcagggcc ctagtgcaca gggaaagcca acctactttc gagaggaaga
     8041 agaagaaatc cacagcccta ccctgctccc ggaggcacag aattgagttt aaacnaatca
     8101 ctagctagtt cgaaggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     8161 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     8221 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     8281 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     8341 atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     8401 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     8461 cccgtcaggc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg tcgatcgact
     8521 ctagctagag gatcgatgcc ccgccccgga cgaactaaac ctgactacga catctctgcc
     8581 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
     8641 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     8701 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     8761 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     8821 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     8881 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     8941 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     9001 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     9061 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     9121 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     9181 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     9241 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     9301 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     9361 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     9421 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     9481 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     9541 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     9601 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     9661 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     9721 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     9781 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     9841 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     9901 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     9961 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
    10021 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
    10081 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
    10141 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
    10201 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
    10261 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
    10321 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
    10381 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
    10441 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
    10501 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
    10561 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
    10621 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgcatataca gtcagcatat
    10681 gatacccagt agtagagtgg gagtgctatc ctttgcatat gccgccacct cccaaggggg
    10741 cgtgaatttt cgctgcttgt ccttttcctg ctggttgctc ccattcttag gtgaatttaa
    10801 ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt
    10861 ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg ggtatgccca
    10921 attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa atacaccaac
    10981 agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca cggcttttaa
    11041 tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca ccctcatctc
    11101 catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc ccttccacca
    11161 taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca gtcaccctga
    11221 tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct
    11281 cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac tcccttagcg
    11341 ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc aatggtgtaa
    11401 gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg gaggtcttac
    11461 tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc ctttctacgt
    11521 gactcctagc caggagagct cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac
    11581 ctccttgacc acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct
    11641 gaccccgggg tccagtgctt gggccttctc ctgggtcatc tgcggggccc tgctctatcg
    11701 ctcccggggg cacgtcaggc tcaccatctg ggccaccttc ttggtggtat tcaaaataat
    11761 cggcttcccc tacagggtgg aaaaatggcc ttctacctgg agggggcctg cgcggtggag
    11821 acccggatga tgatgactga ctactgggac tcctgggcct cttttctcca cgtccacgac
    11881 ctctccccct ggctctttca cgacttcccc ccctggctct ttcacgtcct ctaccccggc
    11941 ggcctccact acctcctcga ccccggcctc cactacctcc tcgaccccgg cctccactgc
    12001 ctcctcgacc ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg
    12061 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
    12121 ctgcccctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc
    12181 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
    12241 cccctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc
    12301 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgctcctg
    12361 cccctcctgc ccctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
    12421 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc
    12481 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
    12541 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc
    12601 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctgcccctc
    12661 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg
    12721 ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg
    12781 gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc
    12841 ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc
    12901 tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc
    12961 catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat
    13021 agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca
    13081 acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat
    13141 tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc
    13201 cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc
    13261 ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg
    13321 tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat
    13381 cagcttatcg atgataagct gtcaaacatg agaattaatt cttgaagacg aaagggcctc
    13441 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
    13501 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
    13561 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
    13621 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
    13681 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
    13741 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
    13801 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
    13861 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    13921 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    13981 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    14041 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    14101 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    14161 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    14221 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    14281 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    14341 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    14401 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    14461 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    14521 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    14581 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    14641 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    14701 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    14761 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
    14821 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    14881 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    14941 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    15001 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    15061 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    15121 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    15181 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    15241 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttgaagc
    15301 tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg
    15361 atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcg
//
