LOCUS Exported 10924 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION 3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition. ACCESSION . VERSION . KEYWORDS pLenti-CaMKIIa-eNpHR-EYFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10924) AUTHORS Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, Bamberg E, Nagel G, Gottschalk A, Deisseroth K TITLE Multimodal fast optical interrogation of neural circuitry. JOURNAL Nature. 2007 Apr 5. 446(7136):633-9. PUBMED 17410168 REFERENCE 2 (bases 1 to 10924) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10924 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2602..3890 /label=CaMKII promoter /note="promoter for the mouse calcium/calmodulin-dependent kinase II alpha subunit" CDS 4851..5564 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(4893..4914) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5154..5173) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5501..5522 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5616..6204 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5669..5689) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6087..6098) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6207..6223) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6208..6224) /label=pBluescriptKS /note="For pBluescript vector" LTR 6729..6909 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6935..6952) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6941..7165 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7211..7639 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7298..7317) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7508..7529 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7648..7668) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7653..7982 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7833..7968 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7895..7914 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 8030..8077 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 8096..8470 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8600..8721 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8637..8656) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8691..8710 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8770..8786) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8770..8786) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8783..8805) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8794..8810 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8818..8848) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8863..8884 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9001..9018) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9172..9760) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9252..9271) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9931..10791) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10554..10573 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10792..10896) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt 2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag 2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat 2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca 2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg 2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga 2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg 3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga 3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac 3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg 3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa 3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt 3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga 3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc 3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa 3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag 3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac 3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag 3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac 3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg 3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt 3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccc 3901 cgggtaccgg tgccaccatg aggggtacgc ccctgctcct cgtcgtctct ctgttctctc 3961 tgcttcagga cacagagacc ctgcctcccg tgaccgagag tgccgtggcc cttcaagccg 4021 aggttaccca aagggagttg ttcgagttcg tgctgaacga ccctttgctt gcaagcagtc 4081 tctatatcaa catcgcactt gcaggactga gtatactgct gttcgttttt atgacccgag 4141 gactcgatga tccacgggca aaacttattg ctgtgtcaac catccttgtg cctgtcgtca 4201 gcattgcctc ctacactgga ttggcgagcg gcctgacaat ttccgttctt gaaatgccag 4261 cgggccattt tgcagaaggc agctcagtga tgctgggagg agaagaggta gatggtgtag 4321 tcaccatgtg gggacggtat ctcacctggg cactttccac gcccatgatt ctcctcgctc 4381 tgggtctcct ggccggaagc aatgctacaa agctcttcac agctatcact ttcgatatcg 4441 ctatgtgcgt gactggcctt gccgcggccc tgactacctc ctcccacctc atgagatggt 4501 tctggtacgc tatcagttgt gcatgctttc tggtggtctt gtatatcctg ctggtggagt 4561 gggcacagga cgccaaagcc gcgggaaccg ctgacatgtt caataccctg aagctgttga 4621 cagtagtgat gtggctgggg tatccaattg tgtgggctct tggagtcgag ggtatcgcgg 4681 tgttgcccgt tggggtgacg agctggggat attctttcct ggatatcgtg gcaaagtaca 4741 ttttcgcatt cttgctcctg aactatctga cgtcaaacga atctgtcgtg tccggcagca 4801 ttttggatgt tccatctgct tctgggaccc cggctgatga tgcggccgcc gtgagcaagg 4861 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 4921 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 4981 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct 5041 tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct 5101 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 5161 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 5221 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 5281 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 5341 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 5401 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc 5461 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 5521 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagttctgc tacgagaacg 5581 aggtgtaatg agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt 5641 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 5701 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 5761 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 5821 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 5881 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 5941 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 6001 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc 6061 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 6121 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 6181 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg 6241 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac 6301 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt 6361 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 6421 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact 6481 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat 6541 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga 6601 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat 6661 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg 6721 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 6781 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 6841 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 6901 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag 6961 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 7021 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 7081 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 7141 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg 7201 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 7261 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 7321 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 7381 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 7441 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 7501 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 7561 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 7621 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc 7681 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 7741 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 7801 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 7861 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 7921 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 7981 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta 8041 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc 8101 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt 8161 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt 8221 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac 8281 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt 8341 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg 8401 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga 8461 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg 8521 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 8581 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 8641 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 8701 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 8761 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 8821 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 8881 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 8941 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 9001 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 9061 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 9121 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 9181 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 9241 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 9301 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 9361 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 9421 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 9481 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 9541 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 9601 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 9661 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 9721 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 9781 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 9841 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 9901 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 9961 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 10021 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 10081 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 10141 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 10201 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 10261 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 10321 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 10381 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 10441 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 10501 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 10561 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 10621 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 10681 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 10741 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 10801 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 10861 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 10921 tgac //