LOCUS       Exported                 710 bp ds-DNA     linear   SYN 11-JUN-2021
DEFINITION  MESA target chain with dTomato ectodomain, no linker domain, M 
            cleavage sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    dTomato_LD 0_CS M_tTA-BFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     277..296
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(552..575)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ttgttggcat aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg
       61 tgtacctaaa tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt
      121 agcgttatta cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg
      181 gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg
      241 ccaatacaat gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc
      301 ttcgattccg acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa
      361 tctagacatc atctgaaaat acaggttttc aacacaaaga gccactgtca ctagcaagcc
      421 ataacaaaac aggactccag caaccacgac cagtgcccaa aaaagcttct tgtacagctc
      481 gtccatgccg tacaggaaca ggtggtggcg gccctcggag cgctcgtact gttccacgat
      541 ggtgtagtcc tcgttgtggg aggtgatgtc cagcttggtg tccacgtagt agtagccggg
      601 cagttgcacg ggcttcttgg ccatgtagat ggtcttgaac tccaccaggt agtggccgcc
      661 gtccttcagc ttcagggcct ggtggatctc gcccttcagc acgccgtcgc
//
