LOCUS Exported 10877 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION 3rd gen lentiviral expression of VChR1 fused to EYFP driven by CaMKIIa promoter for optogenetic activation. ACCESSION . VERSION . KEYWORDS pLenti-CaMKIIa-VChR1-EYFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10877) AUTHORS Zhang F, Prigge M, Beyriere F, Tsunoda SP, Mattis J, Yizhar O, Hegemann P, Deisseroth K TITLE Red-shifted optogenetic excitation: a tool for fast neural control derived from Volvox carteri. JOURNAL Nat Neurosci. 2008 Jun . 11(6):631-3. PUBMED 18432196 REFERENCE 2 (bases 1 to 10877) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10877 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2602..3891 /label=CaMKII promoter /note="promoter for the mouse calcium/calmodulin-dependent kinase II alpha subunit" regulatory 3913..3922 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4828..5544 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(4870..4891) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5131..5150) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5478..5499 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5569..6157 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5622..5642) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6040..6051) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6160..6176) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6161..6177) /label=pBluescriptKS /note="For pBluescript vector" LTR 6682..6862 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6888..6905) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6894..7118 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7164..7592 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7251..7270) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7461..7482 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7601..7621) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7606..7935 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7786..7921 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7848..7867 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7983..8030 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 8049..8423 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8553..8674 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8590..8609) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8644..8663 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8723..8739) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8723..8739) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8736..8758) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8747..8763 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8771..8801) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8816..8837 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8954..8971) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9125..9713) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9205..9224) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9884..10744) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10507..10526 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10745..10849) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt 2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag 2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat 2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca 2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg 2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga 2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg 3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga 3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac 3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg 3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa 3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt 3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga 3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc 3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa 3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag 3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac 3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaaggagga 3661 gcaggtttta aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa 3721 cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct 3781 gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcagtcaagc cggttctccg 3841 tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag cgggggatcc 3901 ccgggtaccg gtgccaccat ggactatcct gttgctagaa gcctcatagt tcgctaccca 3961 accgacctcg gaaacggcac cgtctgcatg ccaagaggac agtgttactg tgaaggttgg 4021 cttcggagtc gcggcacttc cattgaaaag acaatagcaa ttactcttca gtgggtagtc 4081 tttgctttgt cagtggcttg cctggggtgg tatgcgtatc aagcgtggcg agctacctgc 4141 ggatgggagg aggtttacgt agccttgata gaaatgatga aaagcatcat cgaggccttc 4201 cacgagttcg acagccctgc aacactgtgg ctgtcttcag ggaacggcgt agtttggatg 4261 cggtatggcg aatggctcct cacctgcccg gtccttctga tccatctgag caacctcaca 4321 ggcctgaagg acgattatag caaaaggact atgggcctgt tggtttctga tgtgggatgc 4381 atcgtgtggg gcgcaaccag cgccatgtgt acggggtgga cgaagatcct gttcttcctc 4441 atctcattga gctatggtat gtatacctat tttcatgctg ctaaagttta tatcgaagca 4501 ttccacacag ttccaaaagg gatttgtcga gaactggtcc gagtgatggc ctggacattc 4561 tttgtggctt ggggaatgtt tccagtcctg tttctgctgg gcacggaagg attcggtcat 4621 atcagccctt atggatctgc cattgggcac tccatcctcg acctgattgc aaagaacatg 4681 tggggtgtgc tggggaatta cctgcgcgtc aaaatccacg agcacatcct gttgtatggc 4741 gacatcagaa agaagcagaa aattacgatc gccggccaag agatggaggt tgagacactg 4801 gtggctgaag aggaggacgc ggccgccgtg agcaagggcg aggagctgtt caccggggtg 4861 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 4921 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 4981 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc 5041 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc 5101 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 5161 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 5221 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat 5281 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc 5341 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 5401 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc 5461 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 5521 ggcatggacg agctgtacaa gtaagaattc gatatcaagc ttatcgataa tcaacctctg 5581 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 5641 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 5701 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 5761 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 5821 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 5881 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 5941 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc 6001 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 6061 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 6121 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac 6181 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 6241 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 6301 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 6361 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 6421 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca 6481 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc 6541 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg 6601 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga 6661 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 6721 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 6781 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 6841 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc 6901 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 6961 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 7021 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 7081 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 7141 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 7201 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 7261 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 7321 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 7381 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 7441 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 7501 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 7561 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg 7621 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 7681 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 7741 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 7801 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 7861 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 7921 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac 7981 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga 8041 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg 8101 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact 8161 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg 8221 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt 8281 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg 8341 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact 8401 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct 8461 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 8521 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt 8581 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 8641 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta 8701 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 8761 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 8821 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 8881 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 8941 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 9001 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 9061 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 9121 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 9181 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 9241 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 9301 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 9361 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 9421 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 9481 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 9541 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 9601 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 9661 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 9721 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 9781 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 9841 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 9901 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 9961 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 10021 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 10081 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 10141 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 10201 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 10261 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 10321 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 10381 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 10441 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 10501 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 10561 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 10621 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 10681 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 10741 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 10801 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 10861 gaaaagtgcc acctgac //