LOCUS       Exported                 807 bp ds-DNA     linear   SYN 02-JUN-2021
DEFINITION  U6 promoter driving Ebf.774 (F+E) sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    U6>Ebf.774(F+E)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     786..805
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
       61 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      121 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      181 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      241 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      301 gcgagatcca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      361 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      421 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      481 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      541 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      601 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      661 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      721 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      781 acgggataat accgcgccac atagcag
//
