LOCUS Exported 4585 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Luciferase reporter to measure miR-155 mediated differential repression. Contains IL6R 3' UTR and mutated miR-155 sites. ACCESSION . VERSION . KEYWORDS pIS1 mIL6R SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4585) AUTHORS Nam JW, Rissland OS, Koppstein D, Abreu-Goodger C, Jan CH, Agarwal V, Yildirim MA, Rodriguez A, Bartel DP TITLE Global analyses of the effect of different cellular contexts on microRNA targeting. JOURNAL Mol Cell. 2014 Mar 20;53(6):1031-43. doi: 10.1016/j.molcel.2014.02.013. Epub 2014 Mar 13. PUBMED 24631284 REFERENCE 2 (bases 1 to 4585) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4585 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 1464..1585 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1475..1494) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1529..1548 /label=SV40pA-R /note="SV40 polyA, reverse primer" promoter 1718..1822 /gene="bla" /label=AmpR promoter CDS 1823..2683 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2041..2060) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2854..3442 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3343..3362 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 3517..4268 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" intron 4338..4470 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 4453..4472 /label=pMT2-F /note="Synthetic intron, forward primer" promoter 4515..4533 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 4543..893 /codon_start=1 /product="luciferase from the anthozoan coelenterate Renilla reniformis (sea pansy)" /label=Rluc /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" ORIGIN 1 taactggtcc gcagtggtgg gccagatgta aacaaatgaa tgttcttgat tcatttatta 61 attattatga ttcagaaaaa catgcagaaa atgctgttat ttttttacat ggtaacgcgg 121 cctcttctta tttatggcga catgttgtgc cacatattga gccagtagcg cggtgtatta 181 taccagacct tattggtatg ggcaaatcag gcaaatctgg taatggttct tataggttac 241 ttgatcatta caaatatctt actgcatggt ttgaacttct taatttacca aagaagatca 301 tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca ttatagctat gagcatcaag 361 ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg 421 aatggcctga tattgaagaa gatattgcgt tgatcaaatc tgaagaagga gaaaaaatgg 481 ttttggagaa taacttcttc gtggaaacca tgttgccatc aaaaatcatg agaaagttag 541 aaccagaaga atttgcagca tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc 601 caacattatc atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac 661 aaattgttag gaattataat gcttatctac gtgcaagtga tgatttacca aaaatgttta 721 ttgaatcgga cccaggattc ttttccaatg ctattgttga aggtgccaag aagtttccta 781 atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa 841 tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa taattctagg 901 agctctatac cggtctcgat atcactacta gtgttctaga gtgtctcaat gcacggggca 961 tttctacctg tgtttctgca gcacccccac tgccttgagt ccccagcagt gctgttattt 1021 gcctaacacc tgtagccatc tgccacgcag ccagacgtga aacgctgaga cagagaccat 1081 ttaggttaaa tacgacagct tatcctgctg ggtggggaaa gtaaaaaata tgctggttca 1141 aggcctaaag taaaatgatc aataatgttt gtaacgtaaa tgaaatattt tcaagaaatg 1201 tgtccagggg tagcactggc tatgttgacg aggcctttgg taactcagag agctcttggc 1261 cctgatgggg acttgccctt acgctttctt tatcaggctc tgagttcaca cggagcctct 1321 ggcacttccc tgctgtcttg ggagaaagga aactggttgc cgcggcaggt tgtggaatct 1381 gttgctggaa ccaggctgga agcccacctg gtagtgaaca gggcccagtg gggcaggctg 1441 gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag 1501 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac 1561 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt 1621 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat 1681 cgataaggat ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 1741 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 1801 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 1861 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 1921 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 1981 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 2041 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 2101 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 2161 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 2221 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 2281 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 2341 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 2401 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 2461 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 2521 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 2581 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 2641 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 2701 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 2761 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 2821 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 2881 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 2941 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 3001 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 3061 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 3121 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 3181 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 3241 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 3301 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 3361 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 3421 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 3481 ttgctggcct tttgctcaca tggctcgaca gatctaaatg agtcttcgga cctcgcgggg 3541 gccgcttaag cggtggttag ggtttgtctg acgcgggggg agggggaagg aacgaaacac 3601 tctcattcgg aggcggctcg gggtttggtc ttggtggcca cgggcacgca gaagagcgcc 3661 gcgatcctct taagcacccc cccgccctcc gtggaggcgg gggtttggtc ggcgggtggt 3721 aactggcggg ccgctgactc gggcgggtcg cgcgccccag agtgtgacct tttcggtctg 3781 ctcgcagacc cccgggcggc gccgccgcgg cggcgacggg ctcgctgggt cctaggctcc 3841 atggggaccg tatacgtgga caggctctgg agcatccgca cgactgcggt gatattaccg 3901 gagaccttct gcgggacgag ccgggtcacg cggctgacgc ggagcgtccg ttgggcgaca 3961 aacaccagga cggggcacag gtacactatc ttgtcacccg gaggcgcgag ggactgcagg 4021 agcttcaggg agtggcgcag ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg 4081 gtgtccccgg aagaaatata tttgcatgtc tttagttcta tgatgacaca aaccccgccc 4141 agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg cggtcccagg 4201 tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac 4261 ccgcttaaaa gcttgattct tctgacacaa cagtctcgaa cttaagctgc agaagttggt 4321 cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga 4381 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta 4441 ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat tacagctctt 4501 aaggctagag tacttaatac gactcactat aggctagcca ccatgacttc gaaagtttat 4561 gatccagaac aaaggaaacg gatga //