LOCUS       Exported               16448 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pOTF3-AK (clone3)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16448)
  AUTHORS   Zwaka TP, Thomson JA
  TITLE     Homologous recombination in human embryonic stem cells.
  JOURNAL   Nat Biotechnol. 2003 Mar . 21(3):319-21.
  PUBMED    12577066
REFERENCE   2  (bases 1 to 16448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16448
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gap             2317..2326
                     /estimated_length=10
     protein_bind    2327..2360
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    2421..2454
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             complement(2461..3264)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2572..2591)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3182..3201
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    3300..3762
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             complement(3776..4492)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3818..3839)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4167..4186
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4426..4447
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4489..4498
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    4527..5094
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     gap             5167..5176
                     /estimated_length=10
     primer_bind     complement(5178..5194)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5178..5194)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    6749..6782
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(14260..14280)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(14260..14278)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14299..14315)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14299..14315)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14312..14334)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14323..14339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(14347..14377)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14392..14413
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14530..14547)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14701..15289)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14781..14800)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15460..16320)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16083..16102
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16321..16425)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacc cagaatatct gtagtcagag cacctatcag ttgcaaaagc
      721 catgcctgca accgatggaa aatgtaagag ggagttctta aggttcttgg tggcatcacc
      781 caaggcattc tgggaaaacc tagggcctgg ccccaaaact tccctactct gtggctagtc
      841 ctgctgccaa caaaatcgta gcgacctggc ttttcacagc tttgctttta tttccaagtc
      901 aaggacaagc cgcttcattc actcctgggc atttactctt cttgtgggtc tgtgatattc
      961 cttgctttcc agggagaatg tgcttggcaa ggtctggaga actaattcag aatcttaggg
     1021 gaaggggaga gatggaaata caaacctgct tactggaaag gtgcaaatat atgggttgag
     1081 ctggaggtag gaatacaggt aattaaggtt tctagtttaa gggaaaacag atctattgcc
     1141 atttaaataa ggtaactggg atttggttaa gttcacaaag atagcagaag atttatttac
     1201 aggcttcacc tgtactgtca gggcaagaga aagcctggta aaccagctac agcagtttac
     1261 cagtgtgatg gctgtgacac agctccactc cacgggtgga cacagcagag ggcaactggg
     1321 ctggcctggt tcagtgtgaa tcaaaccgct taacccacac atggtacatg tgattttctt
     1381 ttgtgagcct tacaccaagc caaactattg tcaaagcatc atttctatag aaataaagcc
     1441 ttatcttgac ctgttctatt aaaacctgcc acatccgccc tttcctacct agatttaatg
     1501 agcccaagtt tttttacatg gaagaaatga ctctggggca aagaccccta atgaactagt
     1561 ggcagagcca ggaataaaac ttgagtaact aatgagtcac ttatgggcag agtatgcaaa
     1621 aaccttaagt ggaaaccaaa tagaccctgg tatcaagaaa gcacaaagta ttaatagaag
     1681 tttctggttg gggtgatcta ggttcaacag aaataagatg atttctaagt ataaagccat
     1741 ttaagaattc cagagtaggg tgggaaagca aaaagccagc tctgaacagg taacagctac
     1801 atggtgactg agtctatggg caaaagttct tgcatcacag gcttttggga actagcctat
     1861 cacagggccc tgtacaaata aacttggctg caatcccagc tctccctctg atgttgtgtg
     1921 accttaagga gtgtaaatgg caccttagtt tcagggtcac ttgggtatga gcattggata
     1981 ttcccatccc cacctcagta actgaaggac aaaccaagat aagtgtgtct atctactgtg
     2041 tcccaagctt ctttatttaa gaaaaaagtg atacatgatg tgggattaaa atcaagagca
     2101 tcattgaact tcaccttccc tccaaccagt tgccccaaac tcccctgccc ccaccctttg
     2161 tgttcccaat tccttcctta gtgaatgaag aacttaatcc caaaaaccct ggcacaaact
     2221 ccaggttttc tttccctagc tcctcccctc cccctgtccc ccattcctag aagggcaggc
     2281 accctcgacg gtatcgataa gcttgatatc gaattcnnnn nnnnnnataa cttcgtatag
     2341 catacattat acgaagttat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     2401 attgctttat ttgtaaccat ataacttcgt atagcataca ttatacgaag ttatacgcgt
     2461 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     2521 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     2581 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     2641 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     2701 gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     2761 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     2821 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     2881 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     2941 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     3001 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     3061 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     3121 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     3181 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     3241 atcttgttca atggccgatc ccatattggc tgcaggaatt atcccgggtt gtggcaagct
     3301 tatcatcgtg tttttcaaag gaaaaccacg tccccgtggt tcggggggcc tagacgtttt
     3361 tttaacctcg actaaacaca tgtaaagcat gtgcaccgag gccccagatc agatcccata
     3421 caatggggta ccttctgggc atccttcagc cccttgttga atacgcttga ggagagccat
     3481 ttgactcttt ccacaactat ccaactcaca acgtggcact ggggttgtgc cgcctttgca
     3541 ggtgtatctt atacacgtgg cttttggccg cagaggcacc tgtcgccagg tggggggttc
     3601 cgctgcctgc aaagggtcgc tacagacgtt gtttgtcttc aagaagcttc cagaggaact
     3661 gcttccttca cgacattcaa cagaccttgc attcctttgg cgagagggga aagaccccta
     3721 ggaatgctcg tcaagaagac agggccaggt ttccgggccc tcacattgcc aaaagcttgt
     3781 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
     3841 gatcgcgctt ctcgttgggg tctttgctca gggcggactg gtagctcagg tagtggttgt
     3901 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
     3961 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct
     4021 gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca
     4081 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
     4141 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
     4201 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
     4261 ggtcggggta gcgggcgaag cactgcaggc cgtagccgaa ggtggtcacg agggtgggcc
     4321 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg
     4381 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
     4441 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtggcga
     4501 ccggtggatc ccgggttgtg gcaagcttat catcgtgttt ttcaaaggaa aaccacgtcc
     4561 ccgtggttcg gggggcctag acgttttttt aacctcgact aaacacatgt aaagcatgtg
     4621 caccgaggcc ccagatcaga tcccatacaa tggggtacct tctgggcatc cttcagcccc
     4681 ttgttgaata cgcttgagga gagccatttg actctttcca caactatcca actcacaacg
     4741 tggcactggg gttgtgccgc ctttgcaggt gtatcttata cacgtggctt ttggccgcag
     4801 aggcacctgt cgccaggtgg ggggttccgc tgcctgcaaa gggtcgctac agacgttgtt
     4861 tgtcttcaag aagcttccag aggaactgct tccttcacga cattcaacag accttgcatt
     4921 cctttggcga gaggggaaag acccctagga atgctcgtca agaagacagg gccaggtttc
     4981 cgggccctca cattgccaaa agacggcaat atggtggaaa atcacatata gacaaacgca
     5041 caccggcctt attccaagcg gcttcggcca gtaacgttag ggggggggga gggagaggtt
     5101 aattaagcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     5161 aaccatnnnn nnnnnnggat ccactagttc tagagcggcc gctcagtttg aatgcatggg
     5221 agagcccaga gtggtgacag agacaggggg aaaggcttcc ccctcaggga aagggaccga
     5281 ggagtacagt gcagtgaagt gagggctccc atagcctggg gtaccaaaat ggggccctgg
     5341 ggccagagga aaggacactg gtccccctga gaaaggagac ccagcagcct caaaatcctc
     5401 tcgttgtgca tagtcgctgc ttgatcgctt gcccttctgg cgccggttac agaaccacac
     5461 tcggaccacc tgccagtgaa tgacagaaag gagaatgaca ttagacaatg agctgagaga
     5521 cgggcctgac tctgcttgga cattctatcc aaagccaaca gccctagagc agttagagga
     5581 ggacattaga gaatgagctg agacaggcct gactgcttgg acattctgtc caaagccaac
     5641 agccctagag cagttggagg agccagagct agggaaagcg aggtggtgac aggggaaaga
     5701 gatggagccc gcagagagac atggcactca catccttctc gagcccaagc tgctgggcga
     5761 tgtggctgat ctgctgcagt gtgggtttcg ggcactgcag gaacaaattc tccaggttgc
     5821 ctctcactcg gttctcgata ctggttcgct ttctctttcg ggcctgcacg agggtttctg
     5881 ctttgcatat ctgtgcaggt gggaaggggg tgacaagggc aagctttgga cttgctgagt
     5941 aacagcatca caggggtctg tgactagatg tgtcagcaga gccaggtggt ggtgtgaaaa
     6001 ggcaggatcc tggaagggtt ggctctggac cttatcccag cagaactgag gaatttcact
     6061 ccatcccact gagaaccact gcaccaaaga cggagagcta cgagccagtg atggaagcaa
     6121 tggaaattag gccaagaaag ggaaggtccc cgggtatccc cctcccaccc ttacctcctg
     6181 aagattttca ttgttgtcag cttcctccac ccacttctgc agcaagggcc gcagcttaca
     6241 catgttcttg aagctaagct gcagagcctc aaagcggcag atggtcgttt ggctgaatac
     6301 cttccctggg ggaggccagt caaaagagaa gcaaaatgag ggagcacgca gggcccttgt
     6361 gaccctgaga tccaagctta ccacctcttc ccagagggag ctcaaagccc aagcatcttc
     6421 tccctctccc tactcctctt catgggtgag ggtagagtct gcccctgccc ctccccacta
     6481 ggttcaggga tactccttag aggggagatg cggtcagaat ctgcagaggg gaacccacca
     6541 aatagaaccc ccagggtgag ccccacatcg gcctgtgtat atcccagggt gatcctcttc
     6601 tgcttcagga gcttggcaaa ttgctcgagt tctttctgca gagctttgat gtcctgggac
     6661 tggattttaa aaggcagaag acttgtaaga acataaacac accagttatc aatctcccct
     6721 ttccattcgg gattcaagaa cctacgtgat aacttcgtat agcatacatt atacgaagtt
     6781 attggcccca aggaatagtc tgtagaagtg catctgcctt ccaagctgcc cacctaactt
     6841 ctagaaataa cctacccaca aatgtcattc acccattccc tgttcactga ctcatgcatg
     6901 taacaaagga ctactcttcc cccagaaact ggcacatcca agggatgcag agcatcgtga
     6961 aaggacagaa agagagaccc tggcctcgag gagaacacct gtcaggttat gaaggttaga
     7021 agttctttgc tgggcgcggt ggctcatgcc tataattcca gcactttggg aggccgaggt
     7081 gggcagatca cgaggtcagg agttcaagac cagcatggcc aacatggtga aaccccgtct
     7141 ctactaaaaa cacaaaaatt agctgggcac ggtggcacgc acctgtaatc ccagctactc
     7201 aggaggctga ggcaggagaa tcacttgaac ccgggaggcg gaggttgcag tgagctgaga
     7261 tcacgccact gcactccagc ctgggtgaca gagcaagact ctgtctcaaa gaaaaaaaaa
     7321 aagaagatag ttcatttaat acctgcaaaa ttctctcact caagtatcac ccccagttta
     7381 aggatgtttt gagattagag aaatagataa gctgctaagt tctgggttaa ttaaaaagga
     7441 agagcatcat gtctcagaag ctaaattcag tatatactct ccccagcttg ctttgagggt
     7501 cccacaaact ataacatggc atgcatacac acaaacacag caaaaaagta acaggtgtca
     7561 taagaatgga taaagtgctt tgtgtgtact tactcctcat tttttaaatt gattatccct
     7621 catctttact gtatcttttt cactatagag gcatcctaat tgatttttaa attcaagaga
     7681 tttatcgagc accttctata agccagcggc tatacaaagt ggacaaagag ccctgacatc
     7741 cagcatgaca gaagtgctat tcggcacttg ttcttcaagt tgcccacttg gatctcttcc
     7801 aagtgcactt tccttttttc cctgccctat aactttttaa taataaactt ccactcctgc
     7861 tctgaaaaat aaaaaagtaa ataaaataaa aaatggccag gcacagtggc tcatgtctgt
     7921 aaatcctagc actttgggag gccaaggtgg gcagactgct tgagcccaag agttagaaag
     7981 cagcctgggt aacatagtga gacccgtgcc gccccttctc ccacccctgc tgcctctatt
     8041 taaaaaatat atatatatta tggaaaaaag caaagcagtc cgggcgcagt ggtcatgcct
     8101 gtaatccctt cactttggga ggccaaggtg ggtagatcac ttgaggtcag gagttcaaga
     8161 ctagcctggt caacatagtg agactctgtc tctactaaaa atacaaaaat tagctgggca
     8221 tcatggcgct cccctataat cccagctact caggaggctg gggcaggaga attgcttgaa
     8281 cctaggaggt ggagtttgca gtgagccaag atcgcaccac tgcactccag cctgagggac
     8341 agagtgagac tccatctcaa aaattaaaaa aaaaataaag cagtctatag gagtagggta
     8401 aaggagggaa ggagattatg gaggagggtg acacttttaa agacagagaa ggtgattgtt
     8461 tgagcaaagg acaagagtct aatgtggcaa ggccctgaag tgggccttcc agagcccaaa
     8521 gctggtctgg tggctaggta gatcctgttg cagacatagt gactttgttt tagtccaagt
     8581 gaaatgatct ctcacccttt ttctcccccc ccaagacgga atctcgttct atcgcccagg
     8641 ctggagtgct gtggcgtgat cttggctcac tgcaatctcc gccttctggg ttcaagctat
     8701 tctgcctcag ccgcctgagt agctgggact acaggcaccc accaccatgc ccggctaatt
     8761 tttgtatttt tagtagatat ggggtttcac catgttggcc aggctggtca ggagacctca
     8821 agtgatctgt ccaccttggc ttcccaaagt gctgggatta caggtgtgaa ccaccgcacc
     8881 tagcctcacc tttttttttt ttttttgaga gtttcgcttt tgttgcctag gctggagtgc
     8941 actggcgcga tctcggctca ccgcaaccta catctcccag gttcaagcga ttctcctgcc
     9001 tcagcttcct gagtagctga gattacaggc atgcgtcacc acgcccagct aattttgtat
     9061 ttttagtaga gatggggttt cgccatgttg gtcaggctgg actcgaactc ccaacctcag
     9121 gtgattcgcc tgcctcggcc tcccaaagtg cctggccaca ccttttaaaa cactgactct
     9181 agttgacgtg ttggccacag acagtaggga ggaagcagta taatttgaga agctactgcg
     9241 gtaatcccag cagagatgat ggtggctgag gccagggtta ggttgtgatt gattcaggat
     9301 gtttcttaag gataggatgt aggacgtgaa agaaactgag gatgactggg tttggccttg
     9361 agcaactggg tgatcagggt ggagcagttc agggagccat cacaagagac agaaaacgcg
     9421 gtagtcatct ggtgtctaaa tggcatttaa gccttgaggg tgggtgagag gaaggaaggg
     9481 tagatagagc agaggttgaa ggactgagcc ctggggcatg ccatatgagg ctgccggcgg
     9541 acagaggtgc acagctagtg agaaaaaaac aaggcctttt tgtagtcctg aagcctcaag
     9601 gaagtgtttc aatggtgctt gatcatatca atttcaaata ggctgttttc atccccaact
     9661 tctgctcagc caataactca aactgataaa tgccctctgc tatcctggat tttccaaatt
     9721 ctgttttggg gttttggaat aaacactggt ccaaatcctc gcttcatcat ttagcagtta
     9781 aaacccgtta aataggataa taatacctcc ccctaggaga ttttgtgctg gttaatgaga
     9841 taatgatgta taaacggagc acacagccag gcacttagga agtggaccac aattgccagc
     9901 cattatcatt caaggctcag cagtgacctc ctgcgaagag gttggggctt ctcggtcact
     9961 ccagaaacca gtcacacctt tctgtgaggt ctcaaggctt agtatttaat ctctaattgc
    10021 ttacacttgt cgccttggag gactggaaga tacatcttta atagtcctca gcagggctgg
    10081 atgccttcaa tcccgcagca gctctatatt tgcaaatggc ctggagaaat ctctcaccat
    10141 ttttcttgtt tacaactttg gaactgaggc tgaagtcaat caaaatccag ctttctacaa
    10201 ggggtgccag ggtgtgcacc ttaacacagt ggccagtcat tggcctgagg cagagatccg
    10261 gggaagacaa gccctatact tgactggagg taaacccagc tcacaacgcg cacacacaca
    10321 gcccaaacag gagatcctat cagaaacgag tcacacccta gactttcagg aacaataatc
    10381 ctggaatgag cactgttttt accctcaggc tatgcttaac cctaaggcca aaatcttggg
    10441 tctgataagg gtcaaatttt caagcaggac taagggtggg aaaaggggct caaaccaacc
    10501 ccaagctggg tctggtgctg ggccagtaat gaatgaccag accctgggca ggcctaggag
    10561 atgtgagaga ccctgacaag ggctgggcca gacagagcaa aggccagcct gggccagctt
    10621 ccgactctcc caggccgctc tgccctcacc tgcagttgtc tcttcgaaat ccagcttcca
    10681 gttcccacct ggcccctgcc tgccagggct gcctgcagtt gatacacacc cctccctggc
    10741 cagggcagct gaccctgcct gctcctctcc tgggtgccag gtctgggcag ctgcaggtga
    10801 ccacttcccc atcaggctgc cctgtcatga ccacctcccc acaccccaac cccgtcgaag
    10861 ctcacttgcc tcctccgggt tttgctccag cttctccttc tccagcttca cggcaccagg
    10921 ggtgacggtg cagggctccg gggaggcccc atcggagttg ctctccaccc cgactcctgc
    10981 ttcaccctca ggctgagagg tctccaagcc gccttggggc actagcccca ctccaacctg
    11041 gggcccacag tacgccatcc ccccacagaa ctcatacggc ggggggcatg ggggaatccc
    11101 ccacacctca gagcctggcc caacccccgg cccgattcct ggccctccag gagggccttg
    11161 gaagcttagc caggtccgag gatcaaccca gcccggctcc ggcccccctg gcccatcacc
    11221 tccaccacct ggagggggcg agaaggcgaa atctgaagcc aggtgtcccg ccatggggaa
    11281 ggaaggcgcc ccaagccggg ggcctggtga aatgagggct tgcgaaggga ctactcaacc
    11341 cctctctccc tccccagtcc cacccactag ccttgacctc tggccccgcc ccctggatgg
    11401 gtggaggaga gggaggtggg gggagaaact gaggcgaagg atgtttgcct aatggtggtg
    11461 gcaatggtgt ctgtggaagg ggaaaaccgg gagacacaac tggcgcccct ccaggacctc
    11521 agtgcaggtc ccccacagaa acttttttta tttttatttt ttaagacagg gtctcacttt
    11581 gttgcccaga ctggagtgca gtggagtaca atgatggctc aatgtagcct cgatctactg
    11641 ggccaaagca atccttctgc tccagcctcc taagtggctg ggactacagg cttggaccac
    11701 tgtgccctgt tagttttttt atttttagta gagatggggc cttgctatgt tacccaggct
    11761 ggtcttgaat tcctgtcctc aagaaatcct cccgcctctg ccgcccagtg tcatgattaa
    11821 aggcgtgagc caccacaccc aactttcaac tcccaacccg ctccctggca ctctctcagg
    11881 ctctgcacat cccagctgtc tggaatcact cccacacctc catgttcttc aggaacccag
    11941 gtgcttgacc ccctctccac agacctctgg cactgtgcct tcaggggcca gtcaccctct
    12001 cagctcctca aatttattga atgtgtgtgt ggcgctatcc ctcaatgcat caacagccat
    12061 aagcacaatg gccagctgct cccttatgcc ttcccccgat ccatccagaa tcctaggcat
    12121 tcccatcccg atactggcca aatccagcca ccccgcagcc tgggtgcctg gcaccatctg
    12181 cccagcctgc caaatttcac cccatcttca agagtagact gccagacaag gcctccgtgc
    12241 tatatccccc caccccccat ccccccaccc ctccgtcttc cagaatcaga ctccagactc
    12301 tcctcatcta acagactaag gggttggccc ctacttcccc ttcaagggac cagactttgg
    12361 actgattggg cctcagtttc ccaacctttg ctgaaacaga gtgataagac acccgctttg
    12421 ggccccctcc actatggaac ctgcacatca ggttccttgc tcccctctca accaaaactc
    12481 agacatctaa taccacggta ggccccgttc tccctccccc acctccctgg cccaggcctc
    12541 cagccctagg ccctgggtgg ggaaaaccag ggggtggggg gtgtggagaa aaaatatctg
    12601 acttcaggtt caaagaagcc tgggagggac tgggggaagg gggcaggaca atggccttgg
    12661 ctggacaatc ccggtcccca gagggggcag ctctaaccct aaacaagtgc tcaacccttg
    12721 aatgggcctg gatggctccc ctggggactg cttcctgctc cccaaccccc cagtcccaat
    12781 cccctcacac agaatcccct tcagagacgc taaaaggagc tccagcaacc cccctctgca
    12841 atcccctcaa agactgagcc tcagacgggc accaagggcc ccctacaggg acctaggtat
    12901 ctagttcctc cttcctctgg ggaactcagg cgtccagctt catcgtgcat ccctccccga
    12961 gcccggaaga ttgagggatg tgctttgttt agtggggctg gctggcagaa agacgcagag
    13021 gaggtggcga gtgatttgtg gaggggtgca ggaaggctgc cctaagctcc ccttcagggt
    13081 ctgtttttct gggcctggcc tgagtatcct gaggctcatg ctgctggtct agtgcttgat
    13141 tctgtttgca agagaatagc caacggaatg cctgtctgtg agggatgatg tttgtctgtc
    13201 tgctcccaaa acttgatctc agtggagggc ctggggtaag tctgggggct ccagaggggg
    13261 ctctgggcca gggctcccca cagcttcgaa ggccagaagg ccaggtctgg actgggcacg
    13321 ctgacctctg tcgacttaag taaggcttct cattgcaggc tccaggctca gccctgcctg
    13381 ggcttgtctg ctggggtcag tggctctgtc tgccttctaa ggggatgggt gtcccgtggc
    13441 cagctgtctt catcttggtg gcatccgtga gtcttttgag acttttcccc cactcttatg
    13501 ttgcctctgt tcgtgtgccc atctcctgtc tgtgtagact ttttgagcct aattgtatgc
    13561 gtgcatttca atacctgcca caggtctgcc ggaaggtcta caaggcagtg gggttggagc
    13621 tgtgttcact tctcggcctt taactgccca aaaggcaggt agattatggg gcctggtggg
    13681 ggtgggagga acatgcttcg gaacaggagg aggcccctcc ccagccatct caatccccag
    13741 gacagaacca tcacggcacc tttgtcatgc atctctctgc tgtctgccaa gaagacggcc
    13801 tctcagagga gggggagggg caggcctggg atttggctgg aatctccaca ccagtgtttc
    13861 tcagcttgcc atcctccagg ttccccaaaa gcgctcttcc caagccagtc cagagagtcc
    13921 ctgctgccca ttttcctagt ggctcctaaa acaccttccc caatttcccc actcaacacc
    13981 accctcttgt ttttagatta taatttgtac tgtaggtggt gtatttctgg cctgggcaag
    14041 aggcccattc ccgagaggga cgcagacaag gggtgggtgc ctgggtccct ggctgccttg
    14101 tggctggata tgagcccagt caggggtcag cctcctgcat gcctagactc ctagccggcc
    14161 cccttctggg gtgctcaggg ctgatgggag gttgaggcag gctttccttc cttctcactg
    14221 tccgcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
    14281 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    14341 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    14401 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    14461 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    14521 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    14581 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    14641 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    14701 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    14761 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    14821 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    14881 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    14941 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    15001 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    15061 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    15121 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    15181 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    15241 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    15301 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    15361 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    15421 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    15481 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    15541 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    15601 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    15661 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    15721 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    15781 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    15841 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    15901 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    15961 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    16021 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    16081 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    16141 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    16201 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    16261 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    16321 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    16381 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    16441 agtgccac
//
