LOCUS       Exported                 817 bp ds-DNA     linear   SYN 01-JUN-2021
DEFINITION  An AAV packaging vector that expresses channel rhodopsin 2 (H134R) 
            (fused to EYFP) under the EF1a promoter, which can be deactivated 
            (deleted) by recombination between the flanking loxP sites..
ACCESSION   .
VERSION     .
KEYWORDS    pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 817)
  AUTHORS   McDevitt RA, Tiran-Cappello A, Shen H, Balderas I, Britt JP, Marino 
            RAM, Chung SL, Richie CT, Harvey BK, Bonci A
  TITLE     Serotonergic versus nonserotonergic dorsal raphe projection neurons:
            differential participation in reward circuitry.
  JOURNAL   Cell Rep. 2014 Sep 25;8(6):1857-1869. doi: 
            10.1016/j.celrep.2014.08.037. Epub 2014 Sep 18.
  PUBMED    25242321
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 1, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(63..96)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             complement(108..359)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(150..171)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     499..518
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     758..779
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
       61 tcataacttc gtataatgta tgctatacga agttatggcg cgccttactt gtacagctcg
      121 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
      181 ttctcgttgg ggtctttgct cagggcggac tggtagctca ggtagtggtt gtcgggcagc
      241 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
      301 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
      361 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
      421 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
      481 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
      541 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
      601 tagcgggcga agcactgcag gccgtagccg aaggtggtca cgagggtggg ccagggcacg
      661 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
      721 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
      781 atgggcacca ccccggtgaa cagctcctcg cccttgc
//
