LOCUS       Exported               11697 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  2nd generation lentiviral transfer plasmid. Expresses a synthetic 
            cluster of miR-9/9* and miR-124 fused to Bcl-xL under a 
            doxycycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pTight-9-124-BclxL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Victor MB, Richner M, Hermanstyne TO, Ransdell JL, Sobieski C, Deng 
            PY, Klyachko VA, Nerbonne JM, Yoo AS
  TITLE     Generation of Human Striatal Neurons by MicroRNA-Dependent Direct 
            Conversion of Fibroblasts.
  JOURNAL   Neuron. 2014 Oct 22;84(2):311-23. doi: 10.1016/j.neuron.2014.10.016.
            Epub 2014 Oct 22.
  PUBMED    25374357
REFERENCE   2  (bases 1 to 11697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11697
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        2763..3077
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2771..2789
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2807..2825
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2842..2860
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2878..2896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2914..2932
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2949..2967
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2985..3003
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3050..3074
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4475..5047
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4642..4659)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4869..4888
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5050..5652
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5549..5569
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5668..6256
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5721..5741)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6139..6150)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6463..6696
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6797..6814)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6803..7027
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7073..7501
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7160..7179)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7370..7391
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7510..7530)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7515..7844
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7695..7830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7757..7776
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9052..9173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9222..9238)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9222..9238)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9235..9257)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9246..9262
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9270..9300)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9315..9336
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9453..9470)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9624..10212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9704..9723)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10383..11243)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11006..11025
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11244..11348)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11396..11530
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gagaccggtg gttaacgcgg atcccatcga tgaggccctt tcgtcttcac
     2761 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     2821 agagaacgat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
     2881 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
     2941 cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
     3001 agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
     3061 tgaaccgtca gatcgccagc gctgccacca tgtctcagag caaccgggag ctggtggttg
     3121 actttctctc ctacaagctt tcccagaaag gatacagctg gagtcagttt agtgatgtgg
     3181 aagagaacag gactgaggcc ccagaaggga ctgaatcgga gatggagacc cccagtgcca
     3241 tcaatggcaa cccatcctgg cacctggcag acagccccgc ggtgaatgga gccactggcc
     3301 acagcagcag tttggatgcc cgggaggtga tccccatggc agcagtaaag caagcgctga
     3361 gggaggcagg cgacgagttt gaactgcggt accggcgggc attcagtgac ctgacatccc
     3421 agctccacat caccccaggg acagcatatc agagctttga acaggtagtg aatgaactct
     3481 tccgggatgg ggtaaactgg ggtcgcattg tggccttttt ctccttcggc ggggcactgt
     3541 gcgtggaaag cgtagacaag gagatgcagg tattggtgag tcggatcgca gcttggatgg
     3601 ccacttacct gaatgaccac ctagagcctt ggatccagga gaacggcggc tgggatactt
     3661 ttgtggaact ctatgggaac aatgcagcag ccgagagccg aaagggccag gaacgcttca
     3721 accgctggtt cctgacgggc atgactgtgg ccggcgtggt tctgctgggc tcactcttca
     3781 gtcggaaatg aacgcgtcgg cggcctgtcg gtcccctctg gctctccgcg tgcgcccggg
     3841 gctgcgggag cggagtccgc tcaggccccc gcgccgggtc ggcggcggca cgtggggccc
     3901 acagcgcggc agcggtgccg gcgaatggga ggcccgtttc tctctttggt tatctagctg
     3961 tatgagtgcc acagagccgt cataaagcta gataaccgaa agtagaaatg actctcacaa
     4021 cttctgcgtg cgatggcccg gccggccgcc ctggccacag gagccagagg agcccagaga
     4081 agcccacgcc acccagggat tccagctcga cggagcggcc acgccgggac caggtatccg
     4141 acctcggctc tccttctagt aggtgggagt actgctcaga gctacaactc taggagtagg
     4201 gactccaagc ctagagctcc aagagagggt gaagggcagg gagaaaatta tagtaatagt
     4261 tgcaatgagt cacttgcttc tagatcaaga tcagagactc tgctctccgt gttcacagcg
     4321 gaccttgatt taatgtcata caattaaggc acgcggtgaa tgccaagagc ggagcctaca
     4381 gctgcacttg aaggacatcc gagagaagtt aggaagggtg gggagaaaca attctagaat
     4441 gaacccatcc tgtgcgacac ggccgcaaat tccgcccctc tccctccccc ccccctaacg
     4501 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     4561 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     4621 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     4681 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     4741 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     4801 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     4861 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     4921 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     4981 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     5041 gataatacca tggccaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5101 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5161 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5221 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     5281 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggctc gcgcatggcc
     5341 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5401 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5461 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgctggggtg
     5521 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     5581 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     5641 cccggtgcct gagttcgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
     5701 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     5761 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     5821 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     5881 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     5941 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6001 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6061 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6121 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6181 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     6241 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
     6301 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     6361 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     6421 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
     6481 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     6541 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6601 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6661 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     6721 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgtt
     6781 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     6841 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     6901 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6961 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     7021 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     7081 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7141 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7201 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7261 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7321 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7381 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7441 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7501 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     7561 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     7621 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     7681 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7741 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     7801 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     7861 gtatatccat tttcggatct gatcagcacg tgatgaaaaa gcctgaactc accgcgacgt
     7921 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     7981 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     8041 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     8101 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt aattcagcga gagcctgacc
     8161 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     8221 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     8281 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     8341 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     8401 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     8461 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     8521 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     8581 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     8641 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     8701 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     8761 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     8821 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     8881 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agcacgtgct acgagatttc
     8941 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     9001 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     9061 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     9121 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     9181 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     9241 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     9301 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     9361 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     9421 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     9481 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     9541 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     9601 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     9661 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     9721 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9781 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9841 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9901 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9961 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10021 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    10081 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    10141 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    10201 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    10261 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    10321 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    10381 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    10441 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    10501 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    10561 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    10621 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    10681 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    10741 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    10801 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    10861 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    10921 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    10981 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    11041 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    11101 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    11161 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    11221 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    11281 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    11341 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt
    11401 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    11461 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    11521 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
    11581 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
    11641 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
