LOCUS       Exported                2220 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  FPX biosensor, a new class of genetically encoded fluorescent 
            biosensor based on reversible exchange of the heterodimeric partners
            of green and red dimerization dependent fluorescent proteins 
            (ddFPs)..
ACCESSION   .
VERSION     .
KEYWORDS    single polypeptide FPX biosensor for caspase-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      7..16
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 aagcttgcca ccatggtgag caagagcgag gaggtcatca aagagttcat gcgcttcaag
       61 gtgcgcttgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
      181 ttcgcctggg acatcctgtc cccccagatc atgtacggct ccaaggcgta cgtgaagcac
      241 cccgccgacg tccccgatta catgaagctg tccttccccg agggcttcaa gtgggagcgc
      301 gtgatgcact tcgaggacgg cggtctggtg accgtgactc aggacacctc cctgcaggac
      361 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
      421 atgcagaaga agaccttggg ctgggattat gccaccgagc gcctgtaccc cgaagacggc
      481 gtgctgaagg gcgagcttct ggggcgcctg aagctgaagg acggcggcct caacctggtg
      541 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctacttcgtg
      601 gacaccaagc tgggcatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
      661 cgctccgagg gccgccacca cctggggcat ggtacaggta gcacaggcag cggtagctcc
      721 ggtaccgcct ccggcctcga gagcgctgga ggtagcgcat ctggtggcgg agccaccatc
      781 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
      841 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      901 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagat catgtacggc
      961 tccaaagcgt acgtgaggca ccccgccgac atccccgatt acaagaagct gcccttcccc
     1021 gagggcttca agtgggagcg cgtgatgaac ttcgaagacg gcggtctggt gaccgttacc
     1081 caggactcct ccctgcagga cggcacgctg atctgcaagg tgaagattcg cggcaccaac
     1141 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     1201 atgctgtacc ccgaagacgg cgtgctgaag ggccatagct atcaggccct gaagctgaag
     1261 gacggcggcc actacctggt ggagttcgag accatctaca tggccaagaa gcccgtgcaa
     1321 ctgcccggcg attactgtgt ggacaccaag ctggacatca cctcccacaa cgaggactac
     1381 accatcgtgg aacagtacgg gcgctccgag ggccgccacc gtctgggcat ggacgagcgg
     1441 tacaagggca gcaccggcag cggcagctcc ggatccgcct ccggcgatga ggtggatgga
     1501 gccgtcatca aagagttcat gcgcttcaag gtgcgcttgg agggctccat gaacggccac
     1561 gagttcgaga tcgagggcga gggcgagggc cgcccttacg agggcaccca gaccgccaag
     1621 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc ccccctgatc
     1681 atgtacggct ccaagatgta cgtgaagcac cccgccgacg tccccgatta catgaagctg
     1741 tccttccccg agggcttcaa gtgggagcgc gtgatgcact tcgaggacgg cggtctggtg
     1801 accgcgacac aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc
     1861 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccttggg ctgggattat
     1921 gccaccgagc gcctgtaccc cgaagaaggc gtgctgaagg gcgagctcct ggggcgcctg
     1981 aagctgaagg acggcggcct caacctggtg gagtccaaga ccatctacat ggccaagaag
     2041 cccgtgcaac tgcccggcta ctacttcgtg gacaccaagc tggacatcac ctcccacaac
     2101 gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca cctggggcat
     2161 ggcaccggca gcaccttagc cttgaaatta gcaggtcttg atatcgggag ctagctcgag
//
