LOCUS Exported 10000 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION neomycin resistance; constitutive promoter driving reverse tet activator. ACCESSION . VERSION . KEYWORDS AAVS1-SA-2A-NEO-CAG-RTTA3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10000) AUTHORS Sim X, Cardenas-Diaz FL, French DL, Gadue P TITLE A Doxycycline-Inducible System for Genetic Correction of iPSC Disease Models. JOURNAL Methods Mol Biol. 2015 Jan 29. PUBMED 25630922 REFERENCE 2 (bases 1 to 10000) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10000 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 33..836 /label=HA-L /note="left homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" misc_feature 843..868 /label=SA /note="splice acceptor site" CDS 892..945 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 955..1758 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(1790..1807) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1796..2020 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" enhancer 2026..2405 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2407..2684 /label=chicken beta-actin promoter intron 2686..3703 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 3627..3649 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 3711..3730 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" CDS 3770..4477 /codon_start=1 /product="reverse tetracycline transactivator 3" /label=rtTA3 /note="activates expression from Tet-responsive promoters in the presence of doxycycline" /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPG" primer_bind complement(4567..4586) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 4632..4687 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(4686..4705) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(5048..5064) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 5072..5088 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5096..5126) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5141..5162 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." misc_feature 5236..6072 /label=HA-R /note="right homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" primer_bind complement(6106..6125) /label=T7 /note="T7 promoter, forward primer" promoter complement(6107..6125) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6132..6149) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(6132..6148) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6141..6163) /label=M13/pUC Forward /note="In lacZ gene" CDS 6286..6588 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 6467..6486 /label=ccdB-fwd /note="ccdB gene, forward primer" CDS complement(7985..8845) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8608..8627 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8969..9557 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9458..9477 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9711..9728 /label=L4440 /note="L4440 vector, forward primer" protein_bind 9845..9866 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9881..9911 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9919..9935 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9943..9959 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 9980..9998 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" ORIGIN 1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc 61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca 121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc 181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc 241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc 301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg 361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc 421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc 481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc 541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta 601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag 661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt 721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt 781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc 841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga 901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagatggga 961 tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 1021 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 1081 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 1141 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 1201 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 1261 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 1321 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 1381 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 1441 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc 1501 gacggcgatg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 1561 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 1621 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 1681 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 1741 cttgacgagt tcttctgatc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg 1801 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 1861 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 1921 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 1981 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg gtctcgacat tgattattga 2041 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc 2101 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat 2161 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc 2221 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc 2281 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt 2341 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta 2401 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac 2461 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg 2521 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga 2581 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc 2641 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcggggagt cgctgcgacg 2701 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact 2761 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta 2821 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct 2881 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt 2941 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc 3001 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg 3061 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg 3121 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt 3181 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc 3241 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc 3301 cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc 3361 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt 3421 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg 3481 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc 3541 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg 3601 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag 3661 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct 3721 ggttattgtg ctgtctcatc attttggcaa agaattcctt aaggccacca tgtctaggct 3781 ggacaagagc aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg 3841 cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca 3901 cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca 3961 tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa 4021 gtcataccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg 4081 cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg 4141 cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg 4201 cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga 4261 ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc 4321 tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga 4381 aagcggcggg ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc 4441 ccttgacgat tttgaccttg acatgctccc cgggtaaacg cgtgaattca ctcctcaggt 4501 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca 4561 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct 4621 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg 4681 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt 4741 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata 4801 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc 4861 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc 4921 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc 4981 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt 5041 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 5101 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 5161 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcg 5221 acagtactaa gctttactag ggacaggatt ggtgacagaa aagccccatc cttaggcctc 5281 ctccttccta gtctcctgat attgggtcta acccccacct cctgttaggc agattcctta 5341 tctggtgaca cacccccatt tcctggagcc atctctctcc ttgccagaac ctctaaggtt 5401 tgcttacgat ggagccagag aggatcctgg gagggagagc ttggcagggg gtgggaggga 5461 agggggggat gcgtgacctg cccggttctc agtggccacc ctgcgctacc ctctcccaga 5521 acctgagctg ctctgacgcg gctgtctggt gcgtttcact gatcctggtg ctgcagcttc 5581 cttacacttc ccaagaggag aagcagtttg gaaaaacaaa atcagaataa gttggtcctg 5641 agttctaact ttggctcttc acctttctag tccccaattt atattgttcc tccgtgcgtc 5701 agttttacct gtgagataag gccagtagcc agccccgtcc tggcagggct gtggtgagga 5761 ggggggtgtc cgtgtggaaa actccctttg tgagaatggt gcgtcctagg tgttcaccag 5821 gtcgtggccg cctctactcc ctttctcttt ctccatcctt ctttccttaa agagtcccca 5881 gtgctatctg ggacatattc ctccgcccag agcagggtcc cgcttcccta aggccctgct 5941 ctgggcttct gggtttgagt ccttggcaag cccaggagag gcgctcaggc ttccctgtcc 6001 cccttcctcg tccaccatct catgcccctg gctctcctgc cccttcccta caggggttcc 6061 tggctctgct ctaagggcga attcgcggcc gctaaattca attcgcccta tagtgagtcg 6121 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc 6181 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc 6241 cgcaccgatc gcccttccca acagttgcgc agcctatacg tacggcagtt taaggtttac 6301 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac 6361 acgccggggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc 6421 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc 6481 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc 6541 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggcatg 6601 agattatcaa aaaggatctt cacctagatc cttttcacgt agaaagccag tccgcagaaa 6661 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc 6721 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt 6781 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag 6841 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca 6901 agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 6961 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 7021 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 7081 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg 7141 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 7201 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 7261 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 7321 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 7381 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 7441 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat 7501 gcatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 7561 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 7621 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 7681 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaattat 7741 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 7801 ccgcatcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 7861 aaatacattc aaatatgtat ccgctcatga gattatcaaa aaggatcttc acctagatcc 7921 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 7981 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 8041 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 8101 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 8161 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 8221 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 8281 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 8341 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 8401 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 8461 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 8521 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 8581 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 8641 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 8701 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 8761 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 8821 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 8881 agggttattg tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 8941 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 9001 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 9061 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 9121 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 9181 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 9241 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 9301 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 9361 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 9421 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 9481 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 9541 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 9601 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 9661 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 9721 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 9781 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 9841 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 9901 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc 9961 atgattacgc caagctcaga attaaccctc actaaaggga //