LOCUS       Exported                 802 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  Expresses Cre recombinase from the Cbh promoter and one U6-driven 
            sgRNA control targeting LacZ. AAV backbone..
ACCESSION   .
VERSION     .
KEYWORDS    AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F
  TITLE     CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
  JOURNAL   Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 
            10.1016/j.cell.2014.09.014.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     134..153
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        233..308
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        398..683
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt
       61 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
      121 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
      181 tttatatatc ttgtggaaag gacgaaacac cgtgcgaata cgcccacgcg atgttttaga
      241 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
      301 gtcggtgctt ttttgtttta gagctagaaa tagcaagtta aaataaggct agtccgtagc
      361 gcgtgcgcca attctgcaga caaattctag aggtacccgt tacataactt acggtaaatg
      421 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaatagta acgccaatag
      481 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      541 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      601 cctggcattg tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      661 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      721 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      781 cgatgggggc gggggggggg gg
//
