LOCUS Exported 4688 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS zWnt5b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4688) AUTHORS Stoick-Cooper CL, Weidinger G, Riehle KJ, Hubbert C, Major MB, Fausto N, Moon RT TITLE Distinct Wnt signaling pathways have opposing roles in appendage regeneration. JOURNAL Development. 2007 Feb . 134(3):479-89. PUBMED 17185322 REFERENCE 2 (bases 1 to 4688) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4688 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(6..461) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(143..164) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 355..374 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 588..610 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 602..619 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 603..619 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 626..645 /label=T7 /note="T7 promoter, forward primer" promoter 626..644 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 677..693 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 677..693 /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(2457..2473) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2458..2474) /label=pBluescriptKS /note="For pBluescript vector" promoter complement(2503..2521) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(2542..2558) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2542..2558) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2555..2577) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 2566..2582 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2590..2620) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2635..2656 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(2773..2790) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2944..3532) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3024..3043) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3703..4563) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4326..4345 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4564..4668) /gene="bla" /label=AmpR promoter ORIGIN 1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct 481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca 661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tccctaacaa 721 gtgtgcaaag ccatctttcc ctgaagtctt tcctattgac ggacaagctg ttcaaccaat 781 aaagcacagg tttttgctcg ggatacatct ttcttccatt tcgacaatca acttttatcc 841 aacttccaag aacttccatc tgaggaggcg ctttgcattt caatttcttg gaggaggaag 901 gatggatgtg agaatgaacc aaggacacct acttctggca gtgaccctca tcgtctgcaa 961 ctcacagctg ctggtggtcg ccaactcgtg gtggtcatta gccatgaacc ccatccagag 1021 accggagatg tacatcattg gagcacagcc tctgtgcagc cagctgacgg gcctatctca 1081 gggtcagagg aagctctgcc agctctatca ggaccacatg gtttatattg gagagggggc 1141 gaagacgggc atcaaagagt gccagtatca gttcagacag aggcgatgga actgcagtac 1201 agtggacaac acgtcagtgt tcggccgcgt catgcatata ggcagccgag aaacagcttt 1261 tacgtacgcc gtcagcgcag cgggtgttgt gaatgctgtg agtcgagcgt gccgtgaggg 1321 tgagctttcc acctgcggct gcagtcgagc ggctcgtccc agagacctgc ccagagactg 1381 gctgtggggc ggctgcgggg acaacgtcaa ctatggctac cgcttcgccc gggagtttgt 1441 ggacgctcgt gaacgtgaga agaactaccc acgcggatcg gtggaacacg cacgcacgct 1501 tatgaatctg cagaacaatg aagccggaag aatggcggtg tataatctag cgaatgtggc 1561 ctgcaagtgt catggcgtct caggctcgtg cagcttgaaa acctgctggc tccagctggc 1621 cgacttccgg cgtgttggag aattcctgaa ggagaaatac gacagcgccg ccgccatgcg 1681 cattaaccga cgtggaaaac tagagctggt caataatcga ttcaacccac cgacaggtga 1741 agatctggtc tacatcgacc ccagcccgga ttactgcctg cgcaatgaaa ccactgggtc 1801 tctgggcacc caaggccgcc tatgcaacaa gacctcggag ggtatggacg gctgcgagct 1861 catgtgctgc ggccgcgggt acgaccagtt caagacctac aaacatgagc gctgccactg 1921 caagttccac tggtgctgct atgtcaagtg caaacgctgc acgtcactcg tagaccagtt 1981 tgtgtgcaag tagcagacgt gagaactggg ggacagacgc actgagcaat taagcaggag 2041 aagaaacggg acccttacgg acccagaggg gcaacttaga gataattaaa tgtaaaaatg 2101 atatattaaa tagcaacaaa ttaaaagtat ataaataagt gtacgtgtgc cgttgatagt 2161 aatttaatga cctgaggatc cacgttggac gtcattgatg aaaaaggagg caccggtgtg 2221 atgcattccg gctcaagctg tgtttctctt atatagagag gaaggaactc ttgacttgtg 2281 ctacaagaaa ctctttagag actcgaggaa aaggcgcaga aataggatgg ggaacatcaa 2341 gggcgcatca cccacccatt gccttccaat tcttaaacac acacacacac acacacactc 2401 caccttgctg atgtcagagc tcttaagaac tttcggaatg cggatatcaa gcttatcgat 2461 accgtcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt gagggttaat 2521 ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 2581 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 2641 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 2701 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 2761 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 2821 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 2881 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 2941 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 3001 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 3061 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 3121 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 3181 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 3241 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 3301 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 3361 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 3421 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 3481 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 3541 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 3601 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 3661 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 3721 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 3781 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 3841 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 3901 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 3961 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 4021 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 4081 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 4141 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 4201 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 4261 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 4321 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 4381 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 4441 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 4501 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 4561 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 4621 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 4681 aaaagtgc //