LOCUS Exported 5299 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION MoMLV-based Retroviral vector, expresses mRFP1. ACCESSION . VERSION . KEYWORDS pMXs-mRFP1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5299) AUTHORS Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J TITLE Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. JOURNAL Nat Methods. 2009 May . 6(5):370-6. PUBMED 19404254 REFERENCE 2 (bases 1 to 5299) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5299 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 22..615 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 678..1035 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1094..1510 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1440..1462 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1478..1494 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 1520..1894 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 1832..1848 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" regulatory 1930..1939 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1936..2613 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /note="mammalian codon-optimized" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" primer_bind complement(2066..2084) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 2334..2353 /label=mCherry-F /note="mCherry, forward primer" primer_bind 2528..2551 /label=DsRed1-C /note="DsRed1, forward primer" LTR 2804..3273 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(3403..3420) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3574..4162) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3654..3673) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4326..5186) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4949..4968 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5187..5291) /gene="bla" /label=AmpR promoter ORIGIN 1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta 61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc 121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag 181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat 241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg 301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct 361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg 421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc 481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc 541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag 601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg 661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg 721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg 781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc 841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg 901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct 961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc 1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt 1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa 1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac 1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa 1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc 1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt 1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc 1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc 1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa 1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca 1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca 1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg 1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac 1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc 1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat 1921 ccaccggtcg ccaccatggc ctcctccgag gacgtcatca aggagttcat gcgcttcaag 1981 gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc 2041 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc 2101 ttcgcctggg acatcctgtc ccctcagttc cagtacggct ccaaggccta cgtgaagcac 2161 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc 2221 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac 2281 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta 2341 atgcagaaga agaccatggg ctgggaggcc tccaccgagc ggatgtaccc cgaggacggc 2401 gccctgaagg gcgagatcaa gatgaggctg aagctgaagg acggcggcca ctacgacgcc 2461 gaggtcaaga ccacctacat ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc 2521 gacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag 2581 cgcgccgagg gccgccactc caccggcgcc taatacggcc gctcgataag cttgatgacg 2641 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt 2701 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg 2761 agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac 2821 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct 2881 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 2941 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt 3001 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 3061 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 3121 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata 3181 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg 3241 agtgattgac tacccgtcag cgggggtctt tcacatgcag catgtatcaa aattaatttg 3301 gttttttttc ttaagtattt acattaaatg gccatagttg cattaatgaa tcggccaacg 3361 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 3421 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 3481 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 3541 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 3601 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 3661 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 3721 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 3781 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 3841 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 3901 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 3961 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 4021 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 4081 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 4141 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 4201 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 4261 ctagatcctt ttgcggccgg ccgcaaatca atctaaagta tatatgagta aacttggtct 4321 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 4381 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 4441 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 4501 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 4561 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 4621 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 4681 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 4741 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 4801 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 4861 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 4921 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 4981 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 5041 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 5101 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 5161 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 5221 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 5281 ggggttccgc gcacatttc //