LOCUS       Exported                 829 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  Expression of chimeric yeast beta tubulin (TUB2) - human beta2 
            tubulin Cterminal tail (TUBB2a) C127S, E435C for crosslinking 
            polyglutamate peptide .
ACCESSION   .
VERSION     .
KEYWORDS    pRS424-TUB2 - human TUBB2a Cterminal tail C127S, E435C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Sirajuddin M, Rice LM, Vale RD
  TITLE     Regulation of microtubule motors by tubulin isotypes and 
            post-translational modifications.
  JOURNAL   Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 
            2014 Mar 16.
  PUBMED    24633327
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        26..467
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    26..143
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     438..461
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     482..498
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     482..498
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gggaacaaaa gctggagctc tagtacggat tagaagccgc cgagcgggtg acagccctcc
       61 gaaggaagac tctcctccgt gcgtcctcgt cttcaccggt cgcgttcctg aaacgcagat
      121 gtgcctcgcg ccgcactgct ccgaacaata aagattctac aatactagct tttatggtta
      181 tgaagaggaa aaattggcag taacctggcc ccacaaacct tcaaatgaac gaatcaaatt
      241 aacaaccata ggatgataat gcgattagtt ttttagcctt atttctgggg taattaatca
      301 gcgaagcgat gatttttgat ctattaacag atatataaat gcaaaaactg cataaccact
      361 ttaactaata ctttcaacat tttcggtttg tattacttct tattcaaatg taataaaagt
      421 atcaacaaaa aattgttaat atacctctat actttaacgt caaggagaaa aaaccccgga
      481 ttctagaact agtggatccc cgggcatatg agagaaatca ttcatatctc gacaggtcag
      541 tgtggtaacc aaattggtgc tgcattctgg gaaactatct gtggtgagca cggtttggat
      601 ttcaatggga catatcacgg ccatgacgat atccagaagg agagactgaa cgtgtacttc
      661 aacgaggcat cttctgggaa gtgggttcca agatctatta acgtcgatct agaacctggg
      721 acgattgacg cagtacgcaa ttctgccatc gggaatttgt ttagacctga caattatatc
      781 tttgggcaaa gttctgcggg caacgtgtgg gccaagggtc actacacag
//
