LOCUS Exported 651 bp ds-DNA linear SYN 26-JUN-2021 DEFINITION eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation. ACCESSION . VERSION . KEYWORDS pGL3-Basic-8x-gRNA-eGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 651) AUTHORS Polstein LR, Gersbach CA TITLE A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. JOURNAL Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. PUBMED 25664691 REFERENCE 2 (bases 1 to 651) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 26, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..651 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 251..299 /note="synthetic polyadenylation signal" misc_feature 313..404 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" primer_bind 353..372 /label=RVprimer3 /note="pGL3 vector, forward primer" promoter 611..649 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" ORIGIN 1 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 61 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt 121 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 181 tattacgcca gcccaagcta ccatgataag taagtaatat taaggtacgg gaggtacttg 241 gagcggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga 301 atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa 361 ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat aggtaccgag 421 ctcttacgcg tgctagcccg ggctcgagaa actgcaaaag gtcgagaaac tgcaaaaggt 481 cgagaaactg caaaaggtcg agaaactgca aaaggtcgag aaactgcaaa aggtcgagaa 541 actgcaaaag gtcgagaaac tgcaaaaggt cgagaaactg caaaaggtcg agaaactgca 601 aaagagatct ggtaggcgtg tacggtggga ggtctatata agcagagctc g //