LOCUS Exported 776 bp ds-DNA linear SYN 26-JUN-2021 DEFINITION eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation. ACCESSION . VERSION . KEYWORDS pGL3-Basic-8x-gRNA-eGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 776) AUTHORS Polstein LR, Gersbach CA TITLE A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. JOURNAL Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. PUBMED 25664691 REFERENCE 2 (bases 1 to 776) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 26, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..776 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(167..188) /label=EGFP-C /note="EGFP, forward primer" primer_bind 516..535 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" ORIGIN 1 agcggccggc cgccccgact ctagactcga gcggccgcca ctgtgctgga tatctgcaga 61 attccaccac actggactag tggatccgag ctcggtacca agcttaagtt taaacgctag 121 cttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca 181 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt 241 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt 301 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc 361 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct 421 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca 481 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga 541 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct 601 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga 661 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc 721 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg ttttac //