LOCUS       Exported                 469 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  Expresses CibN-dCas9-CibN in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3.1-CibN-dCas9-CibN
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     A light-inducible CRISPR-Cas9 system for control of endogenous gene 
            activation.
  JOURNAL   Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
  PUBMED    25664691
REFERENCE   2  (bases 1 to 469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gccatggact acaaagacca tgacggtgat
      121 tataaagatc atgacatcga tataaatgga gctataggag gtgacctttt gctcaatttt
      181 cctgacatgt cggtcctaga gcgccaaagg gctcacctca agtacctcaa tcccaccttt
      241 gattctcctc tcgccggctt ctttgccgat tcttcaatga ttaccggcgg cgagatggac
      301 agctatcttt cgactgccgg tttgaatctt ccgatgatgt acggtgagac gacggtggaa
      361 ggtgattcaa gactctcaat ttcgccggaa acgacgcttg ggactggaaa tttcaagaaa
      421 cggaagtttg atacagagac taaggattgt aatgagaaga agaagaaga
//
