LOCUS Exported 769 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION 3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression. ACCESSION . VERSION . KEYWORDS pLenti CMV GFP DEST (736-1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 769) AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. TITLE A versatile viral system for expression and depletion of proteins in mammalian cells. JOURNAL PLoS One. 2009 Aug 6;4(8):e6529. PUBMED 19657394 REFERENCE 2 (bases 1 to 769) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..769 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(45..64) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 166..287 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(177..196) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 231..250 /label=SV40pA-R /note="SV40 polyA, reverse primer" LTR 359..592 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" protein_bind 699..723 /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata 61 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg 121 cgggatgggc ggagttaggg gcgggatagc tagagccaga catgataaga tacattgatg 181 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg 241 atgctattgc tttatttgta accattataa gctgcaataa acaagttcct ctcactctct 301 gatattcatt tctttgcaag ttataaatac tgaataataa gatgacatga actactactg 361 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac 421 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt 481 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca 541 gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgag ctagcccttc cagtcccccc 601 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt 661 accgggccct cgagcggccg ccactgtgct ggatatcaac cactttgtac aagaaagctg 721 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagatttt //