LOCUS Exported 3862 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pJA256 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3862) AUTHORS Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J TITLE MosSCI and gateway compatible plasmid toolkit for constitutive and inducible expression of transgenes in the C. elegans germline. JOURNAL PLoS One. 2011;6(5):e20082. Epub 2011 May 26. PUBMED 21637852 REFERENCE 2 (bases 1 to 3862) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3862 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(624..748) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(798..826) /label=GFP-R /note="GFP, reverse primer. Does NOT anneal to EGFP" primer_bind 1574..1594 /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" terminator 1643..1966 /gene="tbb-2" /label=tbb-2 terminator /note="transcription terminator for the C. elegans tubulin beta-2 gene" protein_bind complement(1971..2066) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2103..2122) /label=T7 /note="T7 promoter, forward primer" promoter complement(2104..2122) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2127..2143) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2127..2143) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2245..2264) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2256..3065 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2323..2342) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3212..3800 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3701..3720 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggagg gaggtggagg tggagctatg agtaaaggag 781 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc 841 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta 901 aatttatttg cactactgga aaactacctg ttccatgggt aagtttaaac atatatatac 961 taactaaccc tgattattta aattttcagc caacacttgt cactactttc tgttatggtg 1021 ttcaatgctt ctcgagatac ccagatcata tgaaacggca tgactttttc aagagtgcca 1081 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga 1141 cacgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcaggtgctg 1201 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta 1261 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat 1321 acatcatggc agacaaacaa aagaatggaa tcaaagttgt aagtttaaac atgattttac 1381 taactaacta atctgattta aattttcaga acttcaaaat tagacacaac attgaagatg 1441 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc 1501 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa 1561 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg 1621 acgaactata caaatgagat aaatgcaaaa tcctttcaag cattcccttc ttctctatca 1681 ctcttctttc tttttgtcaa aaaattctct cgctaattta tttgcttttt taatgttatt 1741 attttatgac tttttatagt cactgaaaag tttgcatctg agtgaagtga atgctatcaa 1801 aatgtgattc tgtctgatgt actttcacaa tctctcttca attccatttt gaagtgcttt 1861 aaacccgaaa ggttgagaaa aatgcgagcg ctcaaatatt tgtattgtgt tcgttgagtg 1921 acccaacaaa aagaggaaac tttattgtgc cgccaagaaa aaagtctcag caactttatt 1981 atacaaagtt ggcattataa aaaagcattg cttatcaatt tgttgcaacg aacaggtcac 2041 tatcagtcaa aataaaatca ttatttggag ctccatggta gcgttaacgc ggccgcgata 2101 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt 2161 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac 2221 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt 2281 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg 2341 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag 2401 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca 2461 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc 2521 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag 2581 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt 2641 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc 2701 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta 2761 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg 2821 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat 2881 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca 2941 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat 3001 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt 3061 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg 3121 acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt 3181 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 3241 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 3301 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 3361 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3421 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3481 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3541 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3601 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3661 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3721 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3781 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3841 gctggccttt tgctcacatg tt //