LOCUS Exported 12405 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Oct4-ires-EGFP(lox neo) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12405) AUTHORS Lengner CJ, Camargo FD, Hochedlinger K, Welstead GG, Zaidi S, Gokhale S, Scholer HR, Tomilin A, Jaenisch R. TITLE Oct4 expression is not required for mouse somatic stem cell self-renewal JOURNAL Cell Stem Cell. 2007 Oct 11;1(4):403-15. PUBMED 18159219 REFERENCE 2 (bases 1 to 12405) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12405 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 13..29 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 13..29 /label=pBluescriptSK /note="For pBluescript vector" regulatory 417..426 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind 2030..2063 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 2151..2262 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind 2251..2268 /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" CDS complement(2300..3103) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2411..2430) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 3021..3040 /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind complement(3165..3184) /label=mPGK-F /note="Mouse PGK promoter, forward primer" primer_bind 3558..3581 /label=MSCV-rev /note="Murine stem cell virus, reverse primer" protein_bind 3608..3641 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS complement(3692..4411) /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS complement(3695..4411) /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3737..3758) /label=EGFP-C /note="EGFP, forward primer" primer_bind 4086..4105 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4345..4366 /label=EGFP-N /note="EGFP, reverse primer" misc_feature 4412..4998 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4585..4604) /label=IRES-F /note="IRES internal ribosome entry site, forward primer" primer_bind 4814..4831 /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind complement(9507..9523) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9508..9524) /label=pBluescriptKS /note="For pBluescript vector" primer_bind complement(9553..9573) /label=T3 /note="T3 promoter, forward primer" promoter complement(9553..9571) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(9592..9608) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9592..9608) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9605..9627) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9616..9632 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9640..9670) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9685..9706 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9823..9840) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9994..10582) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10074..10093) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10753..11613) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11376..11395 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11614..11718) /gene="bla" /label=AmpR promoter rep_origin complement(11744..12199) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(11881..11902) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 12093..12112 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 12326..12348 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 12340..12357 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 12341..12357 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 12367..12386 /label=T7 /note="T7 promoter, forward primer" promoter 12367..12385 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 ggtggcggcc gctctagaac tagtggatcc cccgggctgc aggaattctc catagcacag 61 gggctcgtgg aaaccaagca tggagttggg caggagaaga ctatgagtca aggcacttgc 121 tgccaagcct agcgacctgg acttgaccca caggacccac accatggagg gaaccaactt 181 ttttcctcac attgtgacaa tcttcacaca taaatatgca catacttact gtgtccaggc 241 cagcttggtc ttcatgagat actgggggga ggggagggga gggaggcaga gatctggtag 301 ggctgacatt cagcagcaaa aatgctttaa gacttggctt tgggggctgg agagatggct 361 cagcggttaa gagcactgac tgctctgcca gaggtccaga gttcaattcc catcaaccat 421 ggtggctcac aaccatctgt aatgggatcc attgccctct tctggtgtgt ctgaagacag 481 ctcaaataca taaaataaat aacttttttt ttttctaaag gagctgcttg gctcctcctc 541 tctttttttt ttttaattta tttattatat atatatgtaa gtacactgta gttgtcttca 601 gacataccag aagagggcgt cagatctcat tacagatggt tgtgagccac catgtagttg 661 ctgggatttg aactcgggac ctctggcaga gcagtcagtg ctcttaacca ctgagccatc 721 tcaccagccc caaaataaat aacattttaa aaaataaaga cttaagctgg gagtggtggc 781 gcacgcattt gatcccagca ctcactgggg aggcagaggc aagcagattt ctgagttcga 841 ggccagactg gtttacaaag tgagttccag gacatccaga gctatacaga gaaaccctgt 901 ctcgaaaaac caaaaaaata aaataaataa ataaataaat ggttcagcag ttaggagcta 961 tggctagctg gcctgtggtg gtacatgacc ttaatcccag ctcttggaag gcagggatgg 1021 atagactgga tctgagttca gggacagcct ggcctacaga gttccagata gccagggcta 1081 caccgagaaa tgtctcaaaa caaacaaaaa caaaaagcca atggctcttc ttgcagagga 1141 cccaggtttg gttctcagaa cctacatggc tgctcccaac tctctataaa tccagttcct 1201 tgagatctaa agcctttgac ctattctggc attgtagaca tgtgtacaga ccctactccc 1261 agcactacta cgcccaaaaa aggtggtcat cagctagaat agaagcataa caatttagcc 1321 tagagtggca cgggccttta atctcagcac tgcggaggca gaggcaggct gatctgaggc 1381 caacctggtc cgccaggact acacagaact catcttgaag gaaaagaaaa aaaagagttt 1441 gagggctgga gagatggctc agcggttaag agcactgact gctcttccag aggtcctgag 1501 ttcaattccc agcaaccaca tggtggctca caaccatctg taatgggatc ccatgccctc 1561 ttctggtgtg tctagaagac agctacagtg tacccatata aataaaataa atcttaaccc 1621 ccccaaaaaa actctttttt ttaaagtata agcccaccag aactcccaga gtgacaagag 1681 gaaagaaggc ggctgccaga caatggctat gaggtgatgt ccatcagcta gcagagactt 1741 accgcctcta ggatggcgaa tatgcaatgc cttcctctat aggttgggct ccaaccccaa 1801 ctatccctgg gcattttgtg gaactccaat ggtttcaatg gcgcctgttt tgaagatcac 1861 ctggggtttg agaatccatt actggctttc tccaaccgca ggctctctag gaactgaagg 1921 aaaacaaaat tcagctatct actgtgtgtc ccagtcttta tttaagaaca aaatgatgag 1981 tgacagacag gccaggctcc tgatcatctg cagaattcgg cttgtactga taacttcgta 2041 tagcatacat tatacgaagt tgttgcctgc tattgtcttc ccaatcctcc cccttgctgt 2101 cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt 2161 cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg 2221 gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcgag 2281 ctctagagaa ttgatcccct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct 2341 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa 2401 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca 2461 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc 2521 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg 2581 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa 2641 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg 2701 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt 2761 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca 2821 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg 2881 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt 2941 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag 3001 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg 3061 gagaacctgc gtgcaatcca tcttgttcaa tggccgatcc catattggct gcaggtcgaa 3121 aggcccggag atgaggaaga ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag 3181 aatgccgggc ctccggagga ccttcgggcg cccgccccgc ccctgagccc gcccctgagc 3241 ccgcccccgg acccacccct tcccagcctc tgagcccaga aagcgaagga gcaaagctgc 3301 tattggccgc tgccccaaag gcctacccgc ttccattgct cagcggtgct gtccatctgc 3361 acgagactag tgagacgtgc tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg 3421 gcggggggga acttcctgac taggggagga gtagaaggtg gcgcgaaggg gccaccaaag 3481 aacggagccg gttggcgcct accggtggat gtggaatgtg tgcgaggcca gaggccactt 3541 gtgtagcgcc aagtgcccag cggggctgct aaagcgcatg ctccagactg ccttgggaaa 3601 agtactgata acttcgtata gcatacatta tacgaagttg ttgaagccga attccagcac 3661 actggcggcc gttactagtg gatcggccgc tttacttgta cagctcgtcc atgccgagag 3721 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt 3781 ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt 3841 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt 3901 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga 3961 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga 4021 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg 4081 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt 4141 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc 4201 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg 4261 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg 4321 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc 4381 cggtgaacag ctcctcgccc ttgctcacca tggttgtggc catattatca tcgtgttttt 4441 caaaggaaaa ccacgtcccc gtggttcggg gggcctagac gtttttttaa cctcgactaa 4501 acacatgtaa agcatgtgca ccgaggcccc agatcagatc ccatacaatg gggtaccttc 4561 tgggcatcct tcagcccctt gttgaatacg cttgaggaga gccatttgac tctttccaca 4621 actatccaac tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt atcttataca 4681 cgtggctttt ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg 4741 gtcgctacag acgttgtttg tcttcaagaa gcttccagag gaactgcttc cttcacgaca 4801 ttcaacagac cttgcattcc tttggcgaga ggggaaagac ccctaggaat gctcgtcaag 4861 aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat ggtggaaaat 4921 aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt aacgttaggg 4981 gggggggagg gagaggggcg gatcaacagc atcactgagc ttctttcccc atcccacccc 5041 ccacccctgt tgtgctttta atccctcctc agtaaaagaa tttaacccca aagctccagg 5101 ttctcttgtc tacctccctt gccttggctc acagcatccc cagggagggc tggtgcctca 5161 gtttgaatgc atgggagagc ccagagcagt gacgggaaca gagggaaagg cctcgccctc 5221 aggaaaaggg actgagtaga gtgtggtgaa gtgggggctt ccatagcctg gggtgccaaa 5281 gtggggacct gggggcagag gaaaggatac agccccccct gggaaaggtg tccctgtagc 5341 ctcatactct tctcgttggg aatactcaat acttgatctt ttgcccttct ggcgccggtt 5401 acagaaccat actcgaacca cctaggatgg gcacagagat ggacatcaga tcataagctc 5461 tgggatgctg cagtcagcgg tggcggcggt gggggtgggg gggtgggggg ggtggggaat 5521 gggaacaggg aaacatccag gtaccacagg gcaggatctt ggcactcaca tccttctcta 5581 gcccaagctg attggcgatg tgagtgatct gctgtaggga gggcttcggg cacttcagaa 5641 acatggtctc cagactccac ctcacacggt tctcaatgct agttcgcttt ctcttccggg 5701 cctgcaccag ggtctccgat ttgcatatct gggcagggga agatgggggc gacaagagca 5761 actttagtat ttgacttggc aacagcccca ccttgagatg ggactgaaga acaaaccgct 5821 aagggattgt gctaagggag cattgactgg atgtggcccc agagccaggg gttactgaaa 5881 ggggctctgg aaggggctgg tagacctagg tctgagcctt caccagcact agacggggtt 5941 ctggggaggg accattgtca ggctggtggg ggcagagggt gactcagaag gagagagcct 6001 gattgattag caaatgttaa ggctctggat tccaattcat agcagcagaa taaggacaca 6061 gaacttctcc tgcgagctcc ggttggagag ggaggagttt caagccagga atggaagcag 6121 cagaagggac tgtgcctggc agacattgca cacatcctgc cactcctcac ctcctgaagg 6181 ttctcattgt tgtcggcttc ctccacccac ttctccagca ggggccgcag cttacacatg 6241 ttcttaaggc tgagctgcaa ggcctcgaag cgacagatgg tggtctggct gaacaccttt 6301 cctgaaaggc cgatcaaaca gagtgagtga ggcagcaaga agggagccca agcccccacc 6361 tctgacagtt caagctacaa gcgtctaatc ctcccttacc tgtggagaag cagctcctaa 6421 gatgccccac ccccttgcgc ctacattaag agccgtgaga tcagaacatg ctgggggaga 6481 cccaccaaag agaacgccca gggtgagccc cacgtcggcc tgggtgtacc ccaaggtgat 6541 cctcttctgc ttcagcagct tggcaaactg ttctagctcc ttctgcaggg ctttcatgtc 6601 ctgggactgt agtgagaaag gcagacttgt cagagtcaca ccagctggga ctgtgaccct 6661 agggtgcccc atgaaaagat ctcagcctcc tcaagggctg gtctgattat ataaatgtta 6721 ttcactctat tcctaggtga catagatggc tctttccccc cccagaacct agccatctac 6781 agtaccacac tacccagagt tctgtgcagt accccagcaa gttcctatct gctcacagat 6841 tactcaaaac cgccccactg aggacaaaat gtcaccctat tctgaagaag ttttaaggct 6901 gcagaattga ccagacgaac gagcatgtaa tccctttact caagaaacac atggctcagc 6961 agttaagctc tagctcttgc tccttcgcct gcttgctgcg tgagactaag taactgctag 7021 atccttggac ttccattcac agctgcgact gaaccattgt tgggaattgg gctgccgact 7081 gtaagtcatc aataaattcc tttactatct agagactgtc cataagttct gtgactctag 7141 agaaccctga ctaatacaga agttggtata cggacttctc ttccggagat ccggagttca 7201 attcccagca accacaaggt ggctcacaac cttctgtagt aggatctgat gccctcttct 7261 ggtgtgtgtc tgaagacagg tacactgtac tcatataaaa aaaaaaaaaa tcaaaagaaa 7321 gaaaggaaga aagaaagtcc caaagtatga cacagtatta gtgaagatat tgaaacaggg 7381 actcactagg aagctctcct gcctcagtgt ctcaagggct ggggtaataa gatccatccc 7441 tctgttcagc tctaaatgaa aacctacaca gcacactcta agtagccaaa tgtccatgcc 7501 ccaggagctg acaacactcc ggagtgcgaa gaaaactcct gctgtactag agtgcgacag 7561 agaaagcaag gcagcaggct agaagcctct ggaggtgaga agccttgagg cccaaaccac 7621 aggaaatcat ctgactcacc ctggatgcgg tactggccgg agggtgcaga ggaccacctc 7681 tgacgcccac aggtgtacag tagcaggcat gcacacacca caaaaaaaaa aaaatttttt 7741 tttttcaaag ttgaaacagg gatatcaatc gatgtatgag caatagaacg gcaggggcac 7801 tcgagggtgt ggctaaggct gccttcttct cagtaataca cagtgatggt tgggaccagg 7861 tggggagggt ataacagatg gccagttgag tgtgctttct aagttgcagc gtgtgaaccg 7921 gacccccccc cccaaaatgg ctgtcggttt ctcaggcttt tctgtctcta acagaagaca 7981 gaaatggagg cagtcatctg gggagatgca agtcagcctt gagggcggaa gaggagcagg 8041 ttgttggagc aaaggtccag gatggagggc tgaggtgctc cacaaaggct ggccaaccca 8101 gggcacagac agaagcagca gccagtgagg cacacctcaa tgccatttca atggaaaagg 8161 attctctcgg cttcagacag actttcatct ccaactgctc ctcaaaagac caaaacttgt 8221 aatcgccctc agcagtgtct ttggcttttc taaatcctat tttgaggatt tgggattagc 8281 ttaactaagg ttcaaatctc ttgcttcatt aagggccatt taagatgtga gataaactat 8341 taatgccttc ctaggggact cccggcggca tacaggacct gagataacac agaaactggc 8401 acttagggag gggactcctg ctacaacaat cgctaagctg tcggaactcc cggcgggcat 8461 cggtatccac tcgcaccttg ttcttaaggc taattgctta cacttgctcc agagtgctgg 8521 ctgtaggcca tcagacacta agcaaggccc gtgcactcac agaatgatct taagatggcc 8581 tggagataaa actcccctac ccaagggtgt ccctttcttg tttacaagga aaggtagaaa 8641 ggctgaaatc gatcagatct gcacctcagg cactacaagg gtctgctcgt gtaaaggtga 8701 ctcatggagt ctgaggctaa agtagacagg ccgtaaactt gactgaaggt gagccctact 8761 ccacacatgg cccaaacaag agctcctatc agcagccagt cacacccaac ctcttcagta 8821 acaataactc caggataagc accatttttt acccccccag ccatgcctaa ccttaaggcc 8881 aagttcctgg gtccgactgt gatccaagtc cccagagggg ctgatatgtt tttttttttt 8941 ggggggggct gattttcacg catcactggt gtacagacag tgatggcatg aagcaagtgt 9001 cgggacacag gccaggccta ggtgacctac atgacaaggg ccaggccgaa acaatccagg 9061 ccggtttctg attcttgcaa ttctgggttc tagtccacac tgcgtcgtgc ttctcaggag 9121 gcccggcagc catcaccacc tttggcaggc agggtggcta ccctacatgt tcccaattcc 9181 cttcactgct gcctctgcca gcccagccaa gtcccttcac ttacctcctc gggagttggt 9241 tccaccttct ccaacttcac ggcattgggg cggtcggcac agggctcaga ggaggttccc 9301 tctgagttgc tttccactcg tgctcctgcc tggccctcag gctgcaaagt ctccacgcca 9361 acttggggga ctaggcccag tccaacctga ggtccacagt atgccatccc tccgcagaac 9421 tcgtatgcgg gcggacatgg ggagatcccc aatacctctg agcctggtcc gattccaggc 9481 ccacctggag gcccttggaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc 9541 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct 9601 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 9661 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 9721 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 9781 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 9841 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 9901 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 9961 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 10021 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 10081 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 10141 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 10201 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 10261 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 10321 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 10381 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 10441 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 10501 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 10561 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 10621 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 10681 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 10741 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 10801 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 10861 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 10921 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 10981 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 11041 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 11101 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 11161 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 11221 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 11281 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 11341 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 11401 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 11461 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 11521 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 11581 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 11641 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 11701 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat 11761 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 11821 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 11881 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 11941 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 12001 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 12061 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 12121 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 12181 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 12241 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 12301 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag 12361 cgcgcgtaat acgactcact atagggcgaa ttggagctcc accgc //