LOCUS       Exported                1007 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Integration-free (episomal) expression of human SOX2 and KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    pCXLE-hSK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 Apr 3. ():.
  PUBMED    21460823
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(300..365)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
ORIGIN
        1 tcgttgaact cctcggtctc tctccgaggt aggggcgcca ggttgctacc gccgcaagcc
       61 gcaccggctc cgccgctctc caggtctgtg gccacggtcg ccgccgccag gtcatagggg
      121 cggccgggaa gcactggggg aagtcgcttc atgtgggaga gctcctcccg ccagcggtta
      181 ttcggggcac ctgcttgacg cagtgtcttc tcccttcccg ccgggccaga cgcgaacgtg
      241 gagaaagatg ggagcagcgc gtcactgaca gccatggtga agggcgaatt gttcatgagt
      301 ggacctggat tgctttctac atccccagcc agtttgagta aatcaaagtt aagagtttgt
      361 ttgacaggag cgacaatttt gagatccccc atgtgtgaga ggggcagtgt gccgttaatg
      421 gccgtgccgg gcaccgggcc gctctggtag tgctgggaca tgtgaagtct gctgggggcg
      481 gcgggttccg gcacctcggc gccggggaga tacatgctga tcatgtcccg gaggtccccg
      541 gcctggcagg gcgccctgga gtgggaggaa gaggtaacca caggggggct ggagctggcc
      601 tcggacttga ccaccgaacc catggagcca agagccatgc caggggtgcc ctgctgcgag
      661 taggacatgc tgtaggtggg cgagccgttc atgtaggtct gcgagctggt catggagttg
      721 tactgcaggg cgctcacgtc gtagcggtgc atgggctgca tctgcgctgc gccgtgcgca
      781 ttgaggcccg ggtgctgcgg gtagcccagc tggtcctgca tcatgctgta gctgccgttg
      841 ctccagccgt tcatgtgcgc gtaactgtcc atgcgctggt tcacgcccgc gcccaggccg
      901 gcgcccaccc cgaccccgct cgccatgcta ttgccgccgg gggccagcag cccgccgggc
      961 agcgtgtact tatccttctt catgagcgtc ttggttttcc gccgggg
//
