LOCUS       Exported                 337 bp ds-DNA     linear   SYN 08-JUL-2021
DEFINITION  Part of Multibow set of constructs--each FP gene is initially not 
            expressed and then adopts a permanent ON or OFF status upon 
            Cre-mediated recombination. . Encodes nuclear EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    pMTB-Multibow-hG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Xiong F, Obholzer ND, Noche RR, Megason SG
  TITLE     Multibow: digital spectral barcodes for cell tracing.
  JOURNAL   PLoS One. 2015 May 26;10(5):e0127822. doi: 
            10.1371/journal.pone.0127822. eCollection 2015.
  PUBMED    26010570
REFERENCE   2  (bases 1 to 337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 8, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    34..67
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACCT). loxN sites are compatible with each 
                     other, but incompatible with loxP or lox2272 sites."
     protein_bind    75..108
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    117..150
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      164..173
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 ttttgcagga tccgccacca tgactagctc gagataactt cgtatagtat accttatacg
       61 aagttatcga gctcataact tcgtatagga tactttatac gaagttataa ctcgagataa
      121 cttcgtatag catacattat acgaagttat accggcagat ctcgccacca tggtgagtgt
      181 gattaaacca gagatgaaga tgaagtactt catggacggc tccgtcaatg ggcatgagtt
      241 cacagttgaa ggtgaaggca caggcaaacc ttacgaggga catcaagaga tgacactacg
      301 cgtcacaatg gccaagggcg ggccaatgcc tttctcg
//
