LOCUS Exported 4475 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pIS1 IMP-1 short UTR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4475) AUTHORS Mayr C, Bartel DP TITLE Widespread shortening of 3'UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells. JOURNAL Cell. 2009 Aug 21. 138(4):673-84. PUBMED 19703394 REFERENCE 2 (bases 1 to 4475) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4475 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 8..759 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" intron 829..961 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 944..963 /label=pMT2-F /note="Synthetic intron, forward primer" promoter 1006..1024 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1034..1969 /codon_start=1 /product="luciferase from the anthozoan coelenterate Renilla reniformis (sea pansy)" /label=Rluc /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" polyA_signal 2430..2551 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2441..2460) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2495..2514 /label=SV40pA-R /note="SV40 polyA, reverse primer" promoter 2684..2788 /gene="bla" /label=AmpR promoter CDS 2789..3649 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3007..3026) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3820..4408 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4309..4328 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct 61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt 121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc 181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc 241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg 301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg 361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac 421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat 481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat 541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt 601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac 661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt 721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca 781 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag 841 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc 901 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca 961 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta 1021 taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac 1081 tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta 1141 ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc 1201 ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc 1261 agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga 1321 tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt 1381 tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa 1441 gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg 1501 gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt 1561 ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa agttagaacc 1621 agaagaattt gcagcatatc ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac 1681 attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat 1741 tgttaggaat tataatgctt atctacgtgc aagtgatgat ttaccaaaaa tgtttattga 1801 atcggaccca ggattctttt ccaatgctat tgttgaaggt gccaagaagt ttcctaatac 1861 tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg 1921 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct 1981 ctataccggt ctcgatatca ctactagtgt tctagaccag cccctccctg tcccttcgag 2041 tccaggacaa caacgggcag aaatcgagag tgtgctctcc ccggcaggcc tgagaatgag 2101 tgggaatccg ggacacctgg gccgggctgt agatcaggtt tgcccacttg attgagaaag 2161 atgttccagt gaggaaccct gatctctcag ccccaaacac ccacccaatt ggcccaacac 2221 tgtctgcccc tcggggtgtc agaaattcta gcgcaaggca cttttaaacg tggattgttt 2281 aaagaagctc tccaggcccc accaagaggg tggatcacac ctcagtggga agaaaaataa 2341 aatttccttc aggttttaaa aacatgcaga gaggtgtttt aatcagcctt aaaggatggt 2401 tcatttgcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac 2461 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt 2521 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt 2581 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg 2641 taaaatcgat aaggatccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 2701 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 2761 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 2821 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 2881 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 2941 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 3001 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 3061 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 3121 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 3181 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 3241 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 3301 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 3361 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 3421 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 3481 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 3541 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 3601 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 3661 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 3721 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 3781 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 3841 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 3901 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 3961 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 4021 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 4081 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 4141 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 4201 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 4261 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 4321 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 4381 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 4441 ggccttttgc tggccttttg ctcacatggc tcgac //