LOCUS       Exported                5814 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses REX-GECO0.9 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    AAV-hSyn-REX-GECO0.9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Wu J, Abdelfattah AS, Miraucourt LS, Kutsarova E, Ruangkittisakul A,
            Zhou H, Ballanyi K, Wicks G, Drobizhev M, Rebane A, Ruthazer ES, 
            Campbell RE
  TITLE     A long Stokes shift red fluorescent Ca(2+) indicator protein for 
            two-photon and ratiometric imaging.
  JOURNAL   Nat Commun. 2014 Oct 31;5:5262. doi: 10.1038/ncomms6262.
  PUBMED    25358432
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    229..250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        265..295
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    303..319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     327..343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     327..343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   381..510
                     /label=AAV2 ITR
     promoter        592..1038
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             1073..1129
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     1825..1844
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2324..2912
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2377..2397)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2795..2806)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2915..2931)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2916..2932)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    2961..3082
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2972..2991)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3026..3045
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3244..3373
                     /label=AAV2 ITR
     primer_bind     complement(3392..3409)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3392..3408)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3401..3423)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3550..4005
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3637..3656)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3847..3868
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4031..4135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4136..4996
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4354..4373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5167..5755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5656..5675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccagatt
      361 taattaaggc cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc
      421 ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg
      481 gagtggccaa ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact
      541 tatctacgta gccatgctct aggaagatct ctgcagaggg ccctgcgtat gagtgcaagt
      601 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccaccca
      661 ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg
      721 ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct
      781 tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca
      841 ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc
      901 ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg
      961 cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg
     1021 tcgtgcctga gagcgcagtc gaattcgggg atccaccatg gtcgactcat cacgtcgtaa
     1081 gtggaataag gcaggtcacg cagtcagagc tataggtcgg ctgagctcac gttgggtttc
     1141 cgagtggatg taccccgagg acggcgccct gaagagcgtg atcaaggagg ggctgaggct
     1201 gaaggacggc ggccactacg ccgccgaggt caggaccacc tacaaggcca aaaagcccgt
     1261 gcagctgccc ggcgcctaca tcgtcgacat caagttggac atcgtgtccc acaacgagga
     1321 ctacaccatc gtggaacagt gcgaacgcgc cgtgggccgc caccccaccg gcggcatggt
     1381 cgagctgtac aagggaggta caggcgggag tctggtgagc aagggcgagg aggataacat
     1441 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     1501 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gaggcctttc agaccgctaa
     1561 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccttcagtt
     1621 catgtacggc tccaaggcct acattaagca cccagccgac atccccgact acttcaagct
     1681 gtccttcccc gagggcttca ggtgggagcg cgtgatgatc ttcgaggacg gcggcattat
     1741 tcacgttgac caggactcct ccctgcagga cggcgtattc atctacaagg tgaagctgcg
     1801 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggagcc
     1861 tacgcgtgac caactgactg aagagcagat cgcagagttt aaagaggctt tctccctatt
     1921 tgacaaggac ggggatggga cgataacaac caaggagctg gggacggtgt tgcggtctct
     1981 ggggcagaac cccacagaag cagagctgca ggacatgatc aatgaagtag atgccgacgg
     2041 tgacggcaca ttcgacttcc ctgagttcct gacgatgatg gcaaggaaaa tgaatgactc
     2101 agacagtgaa gaggaaatta gagaagcgtt ccgcgtgttt gataaggacg gcaatggcta
     2161 catcggcgca gcagagcttc gccacgtgat gacagacctt ggagagaagt taacagatga
     2221 ggaggttgat gaaatgatca gggtagcaga catcgatggg gatggtcagg taaactacga
     2281 agagtttgta caaatgatga cagcgaagta aaagcttatc gataatcaac ctctggatta
     2341 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     2401 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     2461 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     2521 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     2581 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     2641 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     2701 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     2761 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     2821 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     2881 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gacccgggcg
     2941 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     3001 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     3061 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     3121 gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga
     3181 taaggatctt cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact
     3241 acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     3301 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     3361 agcgagcgcg cagccttaat taacctaatt cactggccgt cgttttacaa cgtcgtgact
     3421 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     3481 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     3541 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3601 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3661 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3721 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3781 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3841 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3901 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3961 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     4021 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4081 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4141 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4201 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4261 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4321 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     4381 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     4441 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4501 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     4561 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     4621 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     4681 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     4741 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     4801 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     4861 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     4921 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     4981 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5041 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5101 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5161 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5221 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5281 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5341 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5401 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5461 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5521 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5581 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5641 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5701 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5761 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acat
//
