LOCUS Exported 4289 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pIS1 Cyclin D2 short UTR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4289) AUTHORS Mayr C, Bartel DP TITLE Widespread shortening of 3'UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells. JOURNAL Cell. 2009 Aug 21. 138(4):673-84. PUBMED 19703394 REFERENCE 2 (bases 1 to 4289) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4289 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 8..759 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" intron 829..961 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 944..963 /label=pMT2-F /note="Synthetic intron, forward primer" promoter 1006..1024 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1034..1969 /codon_start=1 /product="luciferase from the anthozoan coelenterate Renilla reniformis (sea pansy)" /label=Rluc /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" polyA_signal 2244..2365 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2255..2274) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2309..2328 /label=SV40pA-R /note="SV40 polyA, reverse primer" promoter 2498..2602 /gene="bla" /label=AmpR promoter CDS 2603..3463 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2821..2840) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3634..4222 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4123..4142 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct 61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt 121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc 181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc 241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg 301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg 361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac 421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat 481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat 541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt 601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac 661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt 721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca 781 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag 841 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc 901 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca 961 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta 1021 taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac 1081 tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta 1141 ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc 1201 ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc 1261 agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga 1321 tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt 1381 tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa 1441 gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg 1501 gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt 1561 ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa agttagaacc 1621 agaagaattt gcagcatatc ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac 1681 attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat 1741 tgttaggaat tataatgctt atctacgtgc aagtgatgat ttaccaaaaa tgtttattga 1801 atcggaccca ggattctttt ccaatgctat tgttgaaggt gccaagaagt ttcctaatac 1861 tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg 1921 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct 1981 ctataccggt ctcgatatca ctactagtgt tctagaggat gccagttggg ccgaaagaga 2041 gagacgcgtc cataatctgg tctcttcttc tttctggttg tttttgttct ttgtgtttta 2101 gggtgaaact taaaaaaaaa attctgcccc cacctagatc atatttaaag atcttttaga 2161 agtgagagaa aaaggtccta cgaaaacgga ataataaaaa gcatttggtg cctatttgaa 2221 gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag 2281 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac 2341 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt 2401 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat 2461 cgataaggat ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 2521 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 2581 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 2641 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 2701 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 2761 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 2821 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 2881 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 2941 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 3001 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 3061 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 3121 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 3181 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 3241 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 3301 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 3361 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 3421 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 3481 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 3541 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 3601 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 3661 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 3721 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 3781 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 3841 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 3901 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 3961 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 4021 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 4081 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 4141 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 4201 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 4261 ttgctggcct tttgctcaca tggctcgac //