LOCUS Exported 9426 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pIS1 Cyclin D2 long-mut-miR15/16 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9426) AUTHORS Mayr C, Bartel DP TITLE Widespread shortening of 3'UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells. JOURNAL Cell. 2009 Aug 21. 138(4):673-84. PUBMED 19703394 REFERENCE 2 (bases 1 to 9426) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9426 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 8..759 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" intron 829..961 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 944..963 /label=pMT2-F /note="Synthetic intron, forward primer" promoter 1006..1024 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1034..1969 /codon_start=1 /product="luciferase from the anthozoan coelenterate Renilla reniformis (sea pansy)" /label=Rluc /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" polyA_signal 7381..7502 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7392..7411) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 7446..7465 /label=SV40pA-R /note="SV40 polyA, reverse primer" promoter 7635..7739 /gene="bla" /label=AmpR promoter CDS 7740..8600 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7958..7977) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8771..9359 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9260..9279 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct 61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt 121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc 181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc 241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg 301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg 361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac 421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat 481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat 541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt 601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac 661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt 721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca 781 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag 841 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc 901 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca 961 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta 1021 taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac 1081 tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta 1141 ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc 1201 ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc 1261 agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga 1321 tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt 1381 tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa 1441 gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg 1501 gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt 1561 ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa agttagaacc 1621 agaagaattt gcagcatatc ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac 1681 attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat 1741 tgttaggaat tataatgctt atctacgtgc aagtgatgat ttaccaaaaa tgtttattga 1801 atcggaccca ggattctttt ccaatgctat tgttgaaggt gccaagaagt ttcctaatac 1861 tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg 1921 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct 1981 ctataccggt ctcgatatca ctactagtgt tctagaggat gccagttggg ccgaaagaga 2041 gagacgcgtc cataatctgg tctcttcttc tttctggttg tttttgttct ttgtgtttta 2101 gggtgaaact taaaaaaaaa attctgcccc cacctagatc atatttaaag atcttttaga 2161 agtgagagaa aaaggtccta cgaaaacgga ctcataaaaa gcatttggtg cctatttgaa 2221 gtacagcata agggaatccc ttgtatatgc gaacagttat tgtttgatta tgtaaaagta 2281 atagtaaaat gcttacagga aaacctgcag agtagttaga gaatatgtat gcctgcaata 2341 tgggaacaaa ttagaggaga cttttttttt tcatgttatg agctagcaca tacaccccct 2401 tgtagtataa tttcaaggaa ctgtgtacgc catttatggc atgattagat tgcaaagcaa 2461 tgaactcaag aaggaattga aataaggagg gacatgatgg ggaaggagta caaaacaatc 2521 tctcaacatg attgaaccat ttgggatgga gaagcacctt tgctctcagc cacctgttac 2581 taagtcagga gtgtagttgg atctctacat taatgtcctc ttgctgtcta cagtagcgga 2641 tacctaaaaa aagatgtttt attttgccag ttggacacag gtgattggct cctgggtttc 2701 atgttctgtg acatcctgct tcttcttcca aatgcagttc attgcagaca ccaccatatt 2761 gctatctaat ggggaaatgt agctatgggc cataaccaaa actcacatga aacggaggca 2821 gatggagacc aagggtggga tccagaatgg agtcttttct gttattgtat ttaaaagggt 2881 aatgtggcct tggcatttct tcttagaaaa aaactaattt ttggtgctga ttggcatgtc 2941 tggttcacag tttagcattg ttataaacca ttccattcga aaagcacttt gaaaaattgt 3001 tcccgagcga tagatgggat ggtttatgca agtcatgctg aatactcctc ccctcttctc 3061 ttttgccccc tcccttcctg cccccagtct gggttactct tcgcttctgg tatctggcgt 3121 tctttggtac acagttctgg tgttcctacc aggactcaag agacacccct tcctgctgac 3181 attcccatca caacattcct cagacaagcc tgtaaactaa aatctgttac cattctgatg 3241 gcacagaagg atcttaattc ccatctctat acttctcctt tggacatgga aagaaaagtt 3301 attgctggtg caaagataga tggctgaaca tcagggtgtg gcattttgtt cccttttccg 3361 tttttttttt ttttattgtt gttgttaatt ttattgcaaa gttgtattca gcgtacttga 3421 atttttcttc ctctccactt cttagaggca ttcagttagc aaagaggttg gagcaacaac 3481 tttttttttt ttttttgcac aattgtaatt gacaggtaat gaagctattt gttaaaatat 3541 ttgccttttt aagtaaaaaa gaaaaatcag aacagggcta tttgaagaat tattttatac 3601 acagattctg ccttgtttca tagtatgagg gttgaagacg gaaaacaatc taagggtctc 3661 tcattttttt aattttgttt tgttcagttt ggtttttttt tttttttgcg cggataagaa 3721 gctaaagtca tccatcctta ttcacgttga cagtacctag ctgtaatgtt tcacagagtg 3781 tgcggatatt ttataaacat ttttataata tattatttta ctgcttaaat tccaagtcct 3841 gaagtagatg gttgagatat gagttcttcg tactggaaaa gcccttccgt agtttgtttt 3901 cttctggtag catattcatg gttgtttttt tttttctttt ttggtttttt ggtttttttt 3961 ttttcctctg atcacattct tcaaagacgg agtattcttt acctcaggtt tactggacaa 4021 aatcaataac tacaaaaggc aatgattcac gcttttgttt tcataatacc tcacaaccgt 4081 acagtttctg cttgggagcc cattcgcatg aggaatacag aagcagtgtg agcagggctg 4141 actccctctc aggtggaagg cagggcggtc tcactcccag ggaccttttt ggtcatggag 4201 gccatcgggc tcccagttag accctggtat cctcatcatg atggaaaaaa tacattgaac 4261 caagggatcc tccctcccct tcaaggcaga cgttcagtac aaacatttat gcggtaggct 4321 cagatgtcgt aatttgcact taggtaccag gtgtcaggaa acagactaaa aagaattcca 4381 ccaggctgtt tggagatcct catcttggag ctttttcaaa agcggggctt catctgcaaa 4441 gggccctttc atcttgaagt ttttcccctc cgtctttccc ctcccctggc atggacacct 4501 tgtgtttagg atcatctctg caggtttcct aggtctgaat ctgcgagtag atgaacctgc 4561 agcaagcagc gtttatggtg cttccttctc cctcctctgt ctcaaactgc gcaggcaagc 4621 actatgcaag cccaggccct ctgctgagcg gtactaaacg gtcgggtttt caatcacact 4681 gaattggcag gataagaaaa ataggtcaga taagtatggg atgatagttg aagggaggtg 4741 aagaggctgc ttctctacag aggtgaaatt ccagatgagt cagtctcttg ggaagtgtgt 4801 ttagaagggt tcaggacttt gtgagttagc atgaccctaa aattctaggg gatttctggt 4861 gggacaatgg gtggtgaatt ttgaagtttt ggagagggaa gtggagcagc cagcaagtaa 4921 gctagccaga gttttctcaa gagccagctt tgctcagcac actctcctgg gccccaagga 4981 gtcccacgga atggggaaag tgggaaccct ggagttcttg ggaatcttgg agcctaaaga 5041 gaaaccgagg tgcaaattca tttcatggtg actgaccctt gagcttaaac agaagcagca 5101 aatgaaagaa ccggacaaat aaggaagggc acaagcctac ccgactctat ttacagtctg 5161 taactttcca ctcttcctgt agtcccgagg cccctgggtc cttctagctt ttctctttcc 5221 catccttggg gccttgtgtg atgatgggtg tggggctgcc gatgggaaag tcgggggttg 5281 ttaggctttt ctgcctgctc ctgcttaaac acaagaagga atcctggatt ttgccctctc 5341 cttagctctt agtctctttg gtaggagttt tgttccagag gagctctccc ccttggattt 5401 gaacttgctc tttttgttgt tgttgttctt tctcttcttt ttcttacctc ccactaaagg 5461 ggttccaaat tatcctggtc tttttctacc ttgttgtgtt tctatctcgt ctttacttcc 5521 atctgtttgt ttttttctcc atcagtgggg gccgagttgt tcccccagcc tgccaaattt 5581 tgatccttcc cctcttttgg ccaaatccta gggggaagaa atcctagtat gccaaaaata 5641 tatgctaagc ataattaaac tccatgcggg tccataacag ccaagaagcc tgcaggagaa 5701 agccaagggc agttccctcc gcagaacacc ccatgcgtgc tgagaggcga gctccttgaa 5761 gaaggggctg ttcttccagg aggccttatt ttgaactgcc tcaggacccc actggagagc 5821 acagcatgcc ttactactgg gtcatccttg gtctatgtgc tctgtactgg aggctctgtt 5881 ctgcctctta tcagccaggt caggggcaca catggcttaa gtgacaaagc cagaggagaa 5941 gacaaccctg acagcatcac gctgcatccc attgctagca ggattggcaa ctcttcagac 6001 ggagctgcgc ttccctgcag tctagcacct ctagggcctc tccagactgt gccctgggag 6061 ctctgggact gaaaggttaa gaacataagg caggatcaga tgactctctc caagagggca 6121 ggggaatttt ctctccatgg gccacagggg acagggctgg gagaagaaat agacttgcac 6181 cttatgtcat gtaaataatt gattttctag ttcaagaaga taatattggt agtgtgggaa 6241 ttggaggtag gaaggggagg aagtctgagt aagccagttg gcttctaagc caaaaggatt 6301 cctctttgtt tatctctgag acagtccaac cttgagaata gctttaaaag ggaaattaat 6361 gctgagatga taaagtcccc ttaagccaac aaaccctctg tagctataga atgagtgcag 6421 gtttctattg gtgtggactc agagcaattt acaagagctg ttcatgcagc catccatttg 6481 tgcaaaatag ggtaagaaga ttcaagagga tatttattac ttcctcatac cacatggctt 6541 ttgatgattc tggattctaa acaacccaga atggtcattt caggcacaac gatactacat 6601 tcgtgtgtgt ctgcttttaa acttggctgg gctatcagac cctattctcg gctcaggttt 6661 tgagaagcca tcagcaaatg tgtacgtgca tgctgtagct gcagcctgca tcccttcgcc 6721 tgcagcctac tttggggaaa taaagtgcct tactgactgt agccattaca gtatccaatg 6781 tcttttgaca ggtgcctgtc cttgaaaaac aaagtttcta tttttatttt taattggttt 6841 agttcttaac tgctggccaa ctcttacatc cccagcaaat catcgggcca ttggattttt 6901 tccattatgt tcatcaccct tatatcatgt acctcagatc tctctctctc tcctctctct 6961 cagttatata gtttcttgtc ttggactttt tttttctttt ctttttcttt ttttttttgc 7021 tttaaaacaa gtgtgatgcc atatcaagtc catgttattc tctcacagtg tactctataa 7081 gaggtgtggg tgtctgtttg gtcaggatgt tagaaagtgc tgataagtag catgatcagt 7141 gtatgcgaaa aggtttttag gaagtatggc aaaaatgttg tattggctat gatggtgaca 7201 tgatatagtc agctgccttt taagaggtct tatctgttca gtgttaagtg atttaaaaaa 7261 ataataacct gttttctgac tagtttaaag atggatttga aaatggtttt gaatgcaatt 7321 aggttatgct atttggacaa taaactcacc ttgacctgcg gccgcttcga gcagacatga 7381 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta 7441 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 7501 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt 7561 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatcca ggtggcactt 7621 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 7681 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 7741 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 7801 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 7861 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 7921 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 7981 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 8041 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 8101 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 8161 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 8221 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 8281 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 8341 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 8401 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 8461 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 8521 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 8581 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 8641 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 8701 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 8761 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 8821 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 8881 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag 8941 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 9001 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 9061 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 9121 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 9181 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 9241 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 9301 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 9361 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg 9421 ctcgac //