LOCUS Exported 942 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Protein expression.. ACCESSION . VERSION . KEYWORDS pCri-8a SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 942) AUTHORS Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, Arolas JL, Gomis-Ruth FX TITLE The pCri System: A Vector Collection for Recombinant Protein Expression and Purification. JOURNAL PLoS One. 2014 Nov 11;9(11):e112643. doi: 10.1371/journal.pone.0112643. eCollection 2014. PUBMED 25386923 REFERENCE 2 (bases 1 to 942) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..942 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 25..47 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 66..83 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 93..113 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS 126..842 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(168..189) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(429..448) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 776..797 /label=EGFP-C /note="EGFP, forward primer" CDS 895..912 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" ORIGIN 1 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat catatgggca 61 gcagccatca tcatcatcat cacagcagcg gcgaaaacct ttacttccag ggcgccatgg 121 ccaaagtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 181 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 241 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 301 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga 361 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 421 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 481 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 541 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 601 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 661 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 721 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 781 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt 841 aaggtaccgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac 901 caccaccacc actgagatcc ggctgctaac aaagcccgaa ag //