LOCUS Exported 1612 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Protein expression.. ACCESSION . VERSION . KEYWORDS pCri-6b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1612) AUTHORS Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, Arolas JL, Gomis-Ruth FX TITLE The pCri System: A Vector Collection for Recombinant Protein Expression and Purification. JOURNAL PLoS One. 2014 Nov 11;9(11):e112643. doi: 10.1371/journal.pone.0112643. eCollection 2014. PUBMED 25386923 REFERENCE 2 (bases 1 to 1612) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1612 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 34..51 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 70..720 /codon_start=1 /product="glutathione S-transferase from Schistosoma japonicum" /label=GST /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPP" primer_bind 681..703 /label=pGEX 5' /note="pGEX vectors, Glutathione-S-transferase, forward primer" CDS 757..777 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS 793..1509 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(835..856) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1096..1115) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1443..1464 /label=EGFP-C /note="EGFP, forward primer" CDS 1562..1579 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" ORIGIN 1 tgtttaactt taagaaggag atataccatg aaacatcacc atcaccatca caacactagt 61 agcaattcca tgtcccctat actaggttat tggaaaatta agggccttgt gcaacccact 121 cgacttcttt tggaatatct tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa 181 ggtgataaat ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa tcttccttat 241 tatattgatg gtgatgttaa attaacacag tctatggcca tcatacgtta tatagctgac 301 aagcacaaca tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga 361 gcggttttgg atattagata cggtgtttcg agaattgcat atagtaaaga ctttgaaact 421 ctcaaagttg attttcttag caagctacct gaaatgctga aaatgttcga agatcgttta 481 tgtcataaaa catatttaaa tggtgatcat gtaacccatc ctgacttcat gttgtatgac 541 gctcttgatg ttgttttata catggaccca atgtgcctgg atgcgttccc aaaattagtt 601 tgttttaaaa aacgtattga agctatccca caaattgata agtacttgaa atccagcaag 661 tatatagcat ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca 721 actagtggat ctggtggtgg tggcggatcc atgagcgaga atctttattt tcagggccat 781 atggctagca aagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 841 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 901 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 961 tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac 1021 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 1081 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 1141 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 1201 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 1261 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 1321 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 1381 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 1441 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 1501 tacaagtaag gtaccggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga 1561 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg ag //