LOCUS Exported 6206 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Middle Entry Vector Containing QS. ACCESSION . VERSION . KEYWORDS pME-QS (L1-L2) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6206) AUTHORS Subedi A, Macurak M, Gee ST, Monge E, Goll MG, Potter CJ, Parsons MJ, Halpern ME TITLE Adoption of the Q transcriptional regulatory system for zebrafish transgenesis. JOURNAL Methods. 2014 Apr 1;66(3):433-40. doi: 10.1016/j.ymeth.2013.06.012. Epub 2013 Jun 20. PUBMED 23792917 REFERENCE 2 (bases 1 to 6206) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6206 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" intron 3546..3611 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 3741..3761 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 4186..4320 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4223..4242) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4277..4296 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" protein_bind complement(4331..4430) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(4447..4466) /label=T7 /note="T7 promoter, forward primer" promoter complement(4448..4466) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4471..4487) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4471..4487) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4589..4608) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 4600..5409 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(4667..4686) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 5556..6144 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6045..6064 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctat gaacaccatc ccggcacgcc atgtcgggga tgtcgccgcc cgcgatcctc 721 tacctctacc gcacatatca tcctccgtcg ccagcggcat gaagcgttcc ttcgcaacca 781 tggccatgct ctacaacgac actggcaaca gcaacgatgt cggtgcccat gccaggcgac 841 caccacgaac cctctccaat agtcggagca cttccgccca cagagtacct ctaggctctt 901 ggtcggcgcc caattccccg ccccgccgtg cgctgccgca tcatcccatc accgccagct 961 tcgatcccga tgcttccatt gttatcgccg gcattcgtgg cgctggcaag tctacgctgg 1021 ccatcatggc atctaccgcc atgaagcgca agatagtcga cctggaatcc gagttccatc 1081 atcttactgg cttgtctagt tccagctaca agaagacaca cggcccggtc gactatggga 1141 ggcgccagat cgccatcttg cagaacatct tgaatctgca caggacccgc gccattctcg 1201 tctgctcttg gctggagcgg gatgtgcagg ccatgttgca ggatttcagc gtgtctaatc 1261 ctgtcattta cgttctgcgc gatgccaaag ctatcgaggc ccatctgaag ggatacgaca 1321 agtccaaagt tggcaccctc cttgatgcca ccagtactgt ccttcgccgc tgcacccgtt 1381 tcgagttctt caacgtctcc gaagagaacc tggacaccca ctccgcttca acatcaccac 1441 ctgctgttcc ggaccagcgg cataccgcgc cgtatctaac gcttaaacga gccgagcgcc 1501 acttcctcaa atttctctcc ttgattctac ccaaggggac catacctttt gtcgagtccg 1561 cctttcccct ggcttccgtc cccgttgaac agcgccgctt tacctacgcc ctcgccttgc 1621 ctgtatctgc cttgctcgac aaaggcgtcg atatccaaga gcttgatgtc ggtgtagacg 1681 caatcgagat cattgtagac gatcttgcaa cgagcgaatc cggcccaacg agccccttgg 1741 gtcttgcgcc ccaccgagcg agcgagatca gtcgtgttgt aggcgaaatc aggagggaca 1801 cagtgatccc catcattctg cacgtggtct ttccagaaag agcgctatat gaagaagctc 1861 tgctcgcgct ttacatgact tacctgaacc atgccttaag gcttgcgcca gattatctta 1921 cggtcgatct ggggctcgat tctggcttgc ttgggcaact aaccaccgtt caaggaacca 1981 ccaaggtcat cggcaataaa caacttgcag aggtcaattc gccgcgctgg ggggatccat 2041 cttggttaca agcctatgaa aaggcccaga atacaggatg cgacttggta aggttgacca 2101 gaccggcttc aaatccccgg gacaacacag acattcggca gttccacgtt gctgtagagg 2161 ccgtcggggg tccaaggctc ccatttattg cttacaacac aggacgccta ggtcggacat 2221 cgatgtgttt taacgagatc ctgactccag ttacaccagt gcctaccaag gaggatgcaa 2281 tcgggctccg caatccagcc catcgctatc tccagcctcc gctcacggct ctggaagcaa 2341 cacaggctct ctactcggca tttgtccacg acccaatgaa gctgtatgtc tttggcgcaa 2401 atgtgggata tagcttgtcc ccagccatgc acaatgccgc actcaaggcc tgtggcattc 2461 cacaccatta caagcccctt tccacagcaa acatcgggac tttgcgcgag gttatcagcg 2521 atccgcagtt tgctggagcc tcggtcggcc tgccgtttaa ggtggaaatc atcagcctca 2581 cacactcgct gagccggcac gcgaaagcca tcggagccgt caacaccttg attccggtac 2641 gacaccttac cgcggacggt ggaataccgg acgaggtgtc catgttcaac aatatcagcc 2701 aagccggcgc tgtcagagct ctctacggcg agaacacgga ttggattggt atccgagcct 2761 gccttcgccg cggtttatcg cccgccaatg ccgtgagatc aacaagcact ggtcttgtca 2821 tcggcgctgg cggaatggct agggcagctg tctatgccat gcttcaactg ggagtcaaga 2881 agattttgat ctttaaccga acatttgcta atgccgagaa gctggttcta cacttcgaga 2941 acctgttggt cagagacgca ttgcctctgt tgagcacagg gccaagatcc cacgacaaca 3001 cctgttttca catcattcga tctcgagacg atccgctccc agaaaacttc aaaaacccga 3061 ccatgatcgt ttcctgcata ccgacacaca cagtggacaa cacccctgac cctgaattta 3121 ctgtgccttt gcactggctc gacaacccca ctggcggcat tgtactagaa ctcgactaca 3181 aatgtctcac atcacccttg ctcgaacaaa cacgacgcga ggctcacaga ggctgggtcg 3241 caatggatgg acttgacctc ttgccagaac aagggtttgc ccaatttgaa ctgttcaccg 3301 ggcggcgagc acctcgtcgc ttgatgaggc gcgaggtttt gcgagcatac ccagatgatc 3361 aagcaaaatc tcataccgcg cagttacagc ctcgcctcaa cggaattgca acgcaaatat 3421 cttgagccta gatccgcgac ccatttgctg tccaccagtc atgctagagg atctttgtga 3481 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct 3541 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt 3601 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta 3661 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg 3721 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc 3781 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct 3841 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat 3901 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta 3961 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta 4021 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag 4081 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac 4141 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca 4201 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 4261 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 4321 ggatccaggc acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca 4381 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct 4441 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc 4501 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata 4561 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa 4621 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct 4681 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg 4741 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 4801 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt 4861 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta 4921 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc 4981 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc 5041 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag 5101 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca 5161 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 5221 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt 5281 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 5341 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 5401 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga 5461 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga 5521 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 5581 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 5641 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 5701 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 5761 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 5821 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 5881 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 5941 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 6001 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 6061 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 6121 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 6181 ttttgctggc cttttgctca catgtt //